期刊论文

Yang, YZ

中文

生物化学与生物物理进展

1000-3282

2003

30

6

940-944

10.3321/j.issn:1000-3282.2003.06.022

湖南省科技厅重点资助项目（0 1SSY 1 0 0 3）~~；

本校为第一且通讯机构

以THP-1巨噬细胞源性泡沫细胞为研究对象,观察肝X受体α(LXRα)在THP-1巨噬细胞源性泡沫细胞胆固醇流出中的调控作用.结果发现,22(R)-羟基胆固醇剂量依赖性增加THP-1巨噬细胞源性泡沫细胞胆固醇流出, 而DIDS剂量依赖性减少THP-1巨噬细胞源性泡沫细胞胆固醇流出.逆转录聚合酶链反应显示, 22(R)-羟基胆固醇可增加THP-1巨噬细胞源性泡沫细胞LXRα mRNA的表达, DIDS可抑制THP-1巨噬细胞源性泡沫细胞LXRα mRNA的表达.结果提示,LXRα在巨噬细胞源性泡沫细胞胆固醇流出中起着重要的调控作用,这为开发和寻找抗动脉粥样硬化药物提供了新的思路.

The liver X receptors (LXR) nuclear receptors are intracellular sensors of cholesterol excess and are activated by various oxysterols. LXRs have been shown to regulate multiple genes of lipid metabolism. After exposure of the cultured THP-1 macrophage-derived foam cells to 22 (R) -hydroxycholesterol and 4, 4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS) respectively at different concentrations for 24 h, cholesterol efflux and liver X receptor a mRNA level were determined by FJ-2107P type liquid scintillator and reverse transcriptase-pol...