期刊论文

Yang, X.-D.

中文

生物化学与生物物理进展

1000-3282

2000

27

2

197-200

10.3321/j.issn:1000-3282.2000.02.023

本校为第一机构

用脱氧核苷酸转移酶介导的dUTP切口末端标记技术(TUNEL法)、流式细胞仪和DNA断裂分析检测细胞凋亡;用免疫组化检测c-fos、c-jun和c-myc蛋白表达,RT-PCR显示c-fos、c-jun和c-myc mRNA表达水平。结果表明ox-LDL可致U937细胞凋亡,其作用具有浓度效应;ox-LDL可以上调c-fos、c-jun和c-myc基因表达,使c-fos、c-jun和c-myc蛋白合成增多,最终诱导U937细胞凋亡。

To investigate the mechanism involved in the apoptosis of human monocyte cell line U937 induced by oxidized low density lipoprotein (ox-LDL). The increase of the degree of cell apoptosis was concentration dependent. The apoptotic cells were detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), flow cytometry and DNA fragment analysis. The protein contents of c-fos, c-jun and c-myc were analyzed by immunochemical staining. The expression of c-fos, c-jun and c-myc mRNA were quantified by reverse transcription-polymerase chain reaction (RT-PCR). The results sh...