A series of new 1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one Derivatives 5 has been designed and regio-selectively synthesized via a tandem aza-Wittig reaction. The structures of all compounds prepared have been confirmed by 1H NMR, IR, EI-MS spectroscopy and elemental analyses. The results of preliminary bioassay indicate that most compounds 5 possess an inhibition effect against Botrytis cinereapers and P...
