期刊论文

The Effect of Hypoxia on the Vigor and Differentiated Ability of Neural Stem Cells from New Born SD Rats

中文

医学临床研究

1671-7171

2007

24

7

1093-1096

10.3969/j.issn.1671-7171.2007.07.015

本校为第一机构

【目的】通过建立缺氧损伤模型,观察缺氧损伤对神经干细胞活力及分化潜能的影响。【方法】采用机械吹打、无血清悬浮培养,95%N2、5%CO2比率的缺氧气体体外分离培养新生大鼠海马神经干细胞,建立缺氧模型,免疫细胞化学鉴定神经干细胞及其分化后的细胞类型,MTT、LDH等方法比较缺氧培养后神经干细胞的变化,并观察缺氧培养1、2、4、6、8 h后神经元分化的比率。【结果】新生大鼠海马可分离出的大量细胞Nestin染色阳性,证实为神经干细胞,分离出的神经干细胞可分化为神经元、胶质细胞等神经细胞类型;95%N2、5%CO2比率的缺氧气体培养6 h后神经干细胞MTT值由缺氧前0.368±0.104降至0.268±0.071（P

[Objective]To isolate and culture NSCs from hippocampus of new born SD rats,establish the hypoxia model,and investigate the regulation of oxygen deficiency on the proliferation and orientating differentiation of NSCs.[Methods]The brain tissues of SD rat were dissociated mechanically.Cell suspensions were cultivated with serum-free culture medium by suspension culture technique in order to acquire large numbers of self-proliferating cell clones.Immunocytochemical stainings of anti-Nestin,NF-L,GFAP were used to identify the cells.NSCs were expose...