期刊论文

中文

中华实验和临床病毒学杂志

1003-9279

2013

27

3

181-183

10.3760/cma.j.issn.1003-9279.2013.03.007

国家自然科学基金项目（81000727）； “十二五”国家科技重大专项（2012ZX10004215）；

本校为第一机构

目的分析GII-4型诺如病毒深圳分离株SZ2010422全基因组序列,了解其分子结构特点及进化特性。方法根据NewOrleans参考株全序列设计引物,用RT-PCR分段扩增诺如病毒全基因组,经分子克隆、测序后进行系统进化分析及衣壳区氨基酸位点分析。结果 GII-4型诺如病毒深圳SZ2010422株病毒基因组全长7559 bp,病毒基因组分三个开放阅读框(ORFs),ORF1、ORF2及 ORF3长度分别为5100 bp, 1623 bp和807 bp, ORF1与ORF2之间有19个核苷酸重叠。深圳株 SZ2010422基因组核苷酸序列与参考株GII-4型New Orleans变异株同源性最高,全长同源性为99.3%, ORFl、ORF2、ORF3同源性分别为99.5%、99.2%和98.6%。根据系统进化分析,...

OBJECTIVE: To obtain information on viral molecular structural and evolutionary characteristics, we conducted the SZ2010422 full-length genomic analysis. METHODS: Primers were designed by New Orleans full sequence, SZ2010422 full genome was amplified by RT-PCR, the whole genome sequence and the capsid domain amino acid sites was analysised after cloned and sequenced. RESULTS: The genome of G II-4 Norovirus SZ2010422 strain was consist of 7559 bp, it revealed three ORFs composites of the whole genome, ORF1 (5100 bp), ORF2 (1623 bp), ORF3 (807 bp) respectively, ORF1 and ORF2 had 19 nucleotide ov...