期刊论文

Microbial community of bacteria and archaea analysis in uranium tailing and uranium-resistant bacteria isolation

Zeng, T.-T.

中文

中国有色金属学报

1004-0609

2018

28

11

2383-2392

10.19476/j.ysxb.1004.0609.2018.11.25

国家自然科学基金资助项目(51408293) 南华大学博士科研启动基金资助项目(2013XQD10) 湖南省研究生科研创新项目(CX2017B535)。

本校为第一且通讯机构

为了考察铀尾矿土壤中耐铀微生物群落结构,通过高通量测序技术解析细菌与古菌群落结构,并从中分离纯化耐铀菌属并进行分子生物学鉴定.通过高通量测序,分别获得42706条细菌和34346条古菌的有效序列.微生物Alpha多样性分析发现,细菌种类、群落丰富度与多样性均高于古菌的.通过细菌群落结构解析,发现Carnobacterium、Enterococcus、Comamondadaceae和Cronobacter这4类菌在铀尾矿土壤中存在,菌属丰度分别为3.14%、2.58%、0.83%和0.53%.其他优势耐铀菌为Bacillus (49.6%)、Lactococcus (31.9%)、Streptococcus (2.87%)、 Caulobacter (0.89%)、Pseudomonas (0.72%)和Enterobacter (0.62%).通过古菌群...

In order to investigate the uranium-resistant microbial communities in uranium tailings, high-throughput sequencing technique was used to analyze the community structure of bacteria and archaea. And isolation and identification of uranium-resistant bacteria were carried out. The results show that, after high-throughput sequencing, sequences of 42706 and 34346 are available for bacteria and archaea with high quality, respectively. Microbial alpha diversity analysis reveals that there are much higher microbial diversity, abundance and richness of bacteria than that of archaea. According to micro...