Rapid and sensitive detection of Shigella spp. and Salmonella spp. by multiple endonuclease restriction real-time loop-mediated isothermal amplification technique
Ministry of Science and Technology, People's Republic of ChinaMinistry of Science and Technology, China [2011ZX10004-001, 2013ZX10004-101]; State Key Laboratory of Infectious Disease Prevention and Control, China CDC [2015SKLID507]
机构署名：
本校为其他机构
院系归属：
医学院
摘要：
Shigella and Salmonella are frequently isolated from various food samples and can cause human gastroenteritis. Here, a novel multiple endonuclease restriction real-time loop-mediated isothermal amplification technology (MERT-LAMP) were successfully established and validated for simultaneous detection of Shigella strains and Salmonella strains in only a single reaction. Two sets of MERT-LAMP primers for 2 kinds of pathogens were designed from ipaH gene of Shigella spp. and invA gene of Salmonella spp., respectively. Under the constant condition ...
