期刊论文

Qin, X.-P.

中文

药学学报

0513-4870

2013

48

1

59-65

10.16438/j.0513-4870.2013.01.014

国家自然科学基金资助项目（30572192,81173060） 南华大学归国人员基金资助项目（2010XQD446）

本校为第一且通讯机构

本研究观察氯沙坦对两肾一夹(2K1C)高血压大鼠体内脯氨酰羧基肽酶(PRCP)-激肽释放酶调节轴的影响,揭示氯沙坦保护肾脏作用的新机制。采用SD大鼠,建立2K1C高血压大鼠模型,分别给予哌唑嗪(5mg·kg~(?1)·d~(?1))或氯沙坦(5、15及45mg·kg~(?1)·d~(?1))连续干预4周,观察血压变化。实验结束后,通过计算大鼠右侧肾脏体重比,石蜡切片HE染色观察肾小球纤维化的程度以及自动生化分析仪检测血清中Na~+、K~+、肌酐以及尿素氮的含量来评估大鼠肾脏功能;RT-PCR分析右侧肾脏中PRCPmRNA的表达;Westernblotting分析肾脏中PRCP、组织激肽释放酶、血浆激肽释放酶和TGF-β1以及血浆中血浆激肽释放酶蛋白的表达水平...

To investigate the effect of losartan on the axis of prolylcarboxypeptidase (PRCP)--kallikrein of the two-kidney, one-clipped (2K1C) hypertensives rats, and explore the novel protection mechanism of losartan on the kidney. Sprague-Dawley (SD) rats were used to develop the 2K1C hypertensive rats. Then, the rats were treated with prazosin (5 mg x kg(-1) x d(-1)) or losartan (5, 15 and 45 mg x kg(-1) x d(-1)) or vehicle, separately. At the same time, the blood pressures were observed. After treated for four weeks, the ratio of right kidney weight and body weight, the change of glomerular morpholo...