Determination of Chloramphenicol Residues in Aquatic Products Using Immunoaffinity Column Cleanup and High Performance Liquid Chromatography with Ultraviolet Detection
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China [201210016, 201210086]; Ministry of Science and Technology [2012BAD33B02]; Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [11275090]; Natural Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [12JJ9006]
机构署名：
本校为第一机构
院系归属：
化学化工学院
摘要：
A method based on HPLC with UV detection was developed for the quantitative determination of chloramphenicol (CAP) residues in aquatic products. The samples were extracted with ethyl acetate–ammonium hydroxide (98 + 2, v/v), followed by a cleanup step using an immunoaffinity column. The analytes were determined by HPLC-UV. Optimal conditions for the extraction and cleanup procedures are described. The linear regression equation was y = 91.47x – 8.60 with R2 = 0.9998 (y = peak area and x = CAP concentration) and showed a good reproducibility. ...
