A Rationally Designed Myoglobin Exhibits a Catalytic Dehalogenation Efficiency More than 1000-Fold That of a Native Dehaloperoxidase

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成果类型：

期刊论文

作者：

Yin, Lu-Lu;Yuan, Hong;Liu, Can;He, Bo;Gao, Shu-Qin;...

通讯作者：

Lin, Ying-Wu

作者机构：

[Yin, Lu-Lu; Lin, Ying-Wu; Liu, Can; He, Bo] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.

[Yuan, Hong; Tan, Xiangshi] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China.

[Yuan, Hong; Tan, Xiangshi] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China.

[Gao, Shu-Qin; Wen, Ge-Bo; Lin, Ying-Wu] Univ South China, Lab Prot Struct & Funct, Hengyang 421001, Peoples R China.

通讯机构：

[Lin, Ying-Wu] U

Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.

Univ South China, Lab Prot Struct & Funct, Hengyang 421001, Peoples R China.

语种：

英文

关键词：

artificial metalloenzyme;dehaloperoxidase;protein design;myoglobin;X-ray structure;EPR

期刊：

ACS CATALYSIS

ISSN：

2155-5435

年：

2018

卷：

期：

页码：

9619-9624

DOI：

10.1021/acscatal.8b02979

基金类别：

collected at Shanghai Synchrotron Radiation Facility (SSRF), China. This work was supported by the National Natural Science Foundation of China (31370812; 21701081) and the double first class construct program of University of South China.

机构署名：

本校为第一且通讯机构

院系归属：

化学化工学院

摘要：

The design of functional metalloenzymes is attractive, but few designs can exceed the catalytic rate of native enzymes. In order to create an effective artificial dehaloperoxidase (DHP), we redesigned the heme center of myoglobin (Mb) by introducing a distal Tyr43 and replacing the distal His64 with an Asp, which combines the structural features of chloroperoxidase (a distal Asp) with DHP (a distal Tyr). The rationally designed F43Y/H64D Mb was shown to exhibit a remarkable dehalogenation activity, with a catalytic efficiency more than 1000-fol...

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A Rationally Designed Myoglobin Exhibits a Catalytic Dehalogenation Efficiency More than 1000-Fold That of a Native Dehaloperoxidase

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