Comparative Proteome Analysis of Human Lung Squamous Carcinoma using Two Different Methods: Two-Dimensional Gel Electrophoresis and iTRAQ Analysis

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Zeng, Gu-Qing;Zhang, Peng-Fei;Li, Cui;Peng, Fang;Li, Mao-Yu;...

通讯作者：

Xiao, Zhi-Qiang

作者机构：

[Li, Guo-Qing; Xu, Yan; Li, Cui; Yi, Hong; Li, Mao-Yu; Xiao, Zhi-Qiang; Zhang, Peng-Fei; Peng, Fang; Zeng, Gu-Qing; Chen, Zhu-Chu] Cent S Univ, Key Lab Canc Prote, Chinese Minist Hlth, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.

[Zeng, Gu-Qing] Univ S China, Dept Gen Surg & Operat Surg, Sch Med, Hengyang 421001, Peoples R China.

[Yu, Feng-Lei; Chen, Ming-Jiu] Cent S Univ, Dept Cardiothorac Surg, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China.

通讯机构：

[Xiao, Zhi-Qiang] C

Cent S Univ, Key Lab Canc Prote, Chinese Minist Hlth, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.

语种：

英文

关键词：

hLSC;LCM;iTRAQ;2-DE;Biomarker.

期刊：

Technology in Cancer Research & Treatment

ISSN：

1533-0346

年：

2012

卷：

期：

页码：

395-408

DOI：

10.7785/tcrt.2012.500287

基金类别：

National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30370642, 30240056, 30973290]; Lotus Scholars Program of Hunan Province, China [2007-362]; scientific Research Fund of Hunan Provincial Education DepartmentHunan Provincial Education Department [09C837]; scientific Research Fund of Hunan Provincial health Department [B2010-037]; scientific Research Fund of Hengyang City Science and Technology Burea [2010kj18]

机构署名：

本校为其他机构

院系归属：

医学院

摘要：

Discovery of early-diagnosis biomarkers is the key to improve the early-diagnosis and prognosis of human lung squamous carcinoma (hLSC). In order to identify more exhaustive and systematic protein biomarkers for early-diagnosis of hLSC, we chose LCM purified cells from hLSC tissues and paired normal bronchial epithelia(NBE) tissues and used two methods, the classical 2-DE/MS approach and the new iTRAQ analysis. We found a total of 63 differential proteins, 22 proteins in 2-DE and 59 proteins in iTRAQ analysis, between hLSC and NBE tissues. Amon...

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Comparative Proteome Analysis of Human Lung Squamous Carcinoma using Two Different Methods: Two-Dimensional Gel Electrophoresis and iTRAQ Analysis

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