版权说明 操作指南
首页 > 成果 > 详情

Simultaneous determination of flavanones, hydroxycinnamic acids and alkaloids in citrus fruits by HPLC-DAD-ESI/MS

认领
导出
Link by DOI
反馈
分享
QQ微信 微博
成果类型:
期刊论文
作者:
He, Dongxiu;Shan, Yang;Wu, Yuehui;Liu, Guozhu;Chen, Bo;Yao, Shouzhuo
通讯作者:
Chen, B.
作者机构:
[He, Dongxiu] Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China
[He, Dongxiu] Department of Pharmacy, School of Life Sciences and Technology, University of South China, Hengyang 421001, China
通讯机构:
[Chen, Bo] Hunan Normal Univ, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, China
语种:
英文
关键词:
Alkaloids;Citrus hybrids;Citrus peel;Citrus pulp;Flavanones;HPLC-DAD-ESI/MS;Hydroxycinnamic acids
期刊:
Food Chemistry
ISSN:
0308-8146
年:
2011
卷:
127
期:
2
页码:
880-885
基金类别:
National "973" project [2006CB504701]; National Natural Science Foundation of China [20875028, 20080542003, 2008[890]]; Science Research Foundation of Hunan province [2007FJ1005, 2008FJ3063]
机构署名:
本校为其他机构
院系归属:
药学与生物科学学院
摘要:
A simple and accurate method has been developed to simultaneously separate and determine 10 bioactive compounds in citrus fruits by high-performance liquid chromatography coupled with diode array detection and electrospray ionisation mass spectrometry (HPLC-DAD-ESI/MS). This HPLC assay was performed on a reversed-phase C18 column with acetonitrile and 0.1% (v/v) aqueous formic acid as mobile phase. DAD has been performed at 273, 283 and 324 nm for quantification of the alkaloids, flavanones and hydroxycinnamic acids. MS was also employed to identify the each analyte. Ten analytes (naringin, hesperidin, neohesperidin, ferulic acid, p-coumaric acid, chlorogenic acid, caffeic acid, octopamine, synephrine and tyramine) demonstrated good linearity (r ≥0.9990) in a relatively wide concentration range. The method revealed high average recovery (range, 92.1-97.9%) and good precision with interday and intraday variations with less than 4.71%. The limits of detection (LODs) ranged from 0.02 to 0.11 μg/ml, while the limits of quantification (LOQs) were defined in the range of 0.08-0.39 μg/ml. The proposed method has been successfully applied to analyse three types of bioactive constituents in eight citrus hybrids pulps and eight citrus hybrids peels, which has been successfully cultivated in China. ©2011 Elsevier Ltd. All rights reserved.

反馈

验证码:
看不清楚,换一个
确定
取消

成果认领

标题:
用户 作者 通讯作者
请选择
请选择
确定
取消

提示

该栏目需要登录且有访问权限才可以访问

如果您有访问权限,请直接 登录访问

如果您没有访问权限,请联系管理员申请开通

管理员联系邮箱:yun@hnwdkj.com