This research was funded by the Provincial Natural Science Foundation of Hunan Province (grant number 2018JJ3452) and the Educational Committee of Hunan Province (grant number 18B269).
机构署名：
本校为第一且通讯机构
院系归属：
医学院
摘要：
Daxx is a highly conserved nuclear protein with an important role in transcription, apoptosis and other cell processes. We investigated the role of HPV16 E6 in Daxx-induced apoptosis through their interactions in C33A cells. The binding of HPV16 E6 and Daxx was confirmed in C33A cells using co-immunoprecipitation and indirect immunofluorescence assays. Quantitative PCR and western blotting were performed to determine the RNA and protein expressions of Daxx, respectively. Automatic cell count and MTT assays were performed to investigate the proliferation of C33A cells. The apoptosis rate of C33...
