期刊论文

Effects of stromal cell-derived factor-1α on proliferation and clone formation of rat bone marrow derived endothelial rrogeultor Cells

中文

中国实用医药

1673-7555

2008

3

21

3-5

10.3969/j.issn.1673-7555.2008.21.002

中国博士后基金（项目编号：2005038472） 湖南省自然科学基金（项目编号：07jj3034） 湖南省教育厅课题（项目编号：07C617） 湖南省自然科学基金（项目编号：06jj5051）

本校为第一机构

目的 观察基质细胞衍生因子-1α(SDF-1α)对体外培养的大鼠骨髓源性内皮祖细胞(EPCs)动员和增殖的影响.方法 微孔法获取大鼠骨髓EPCs并采用荧光显微镜和流式细胞术鉴定EPCs特异性标记物.不同浓度SDF-1α处理EPCs后,采用细胞培养、MTT检测EPCs的克隆形成、细胞增殖.结果 通过MTT法检测,埘照组与(1、10、100 μg/L)SDF-1α处理组的OD值分别为0.311±0.054、0.587±0.072、0.813±0.056、1.029±0.078,通过统计学方法 分析,对照组与SDF-1α处理组比较差异均有统计学意义(n=5,P

Objective To investigate the role of stromal cell-derived factor-1α （SDF-1α）to proliferation and clone formation on endothelial progenitor cells（EPC）. Methods Bone marrow derived EPC were indfined by immunofluorescence staining and flow cytometry. Treated with different concentrations of SDF-1α, the property of proliferation was detected by MMT and clone formation was detected with optical microscopy. Results SDF-1α promoted EPC proliferation and clone formation with concentration-dependent mann...