期刊论文

中文

中国实用医药

1673-7555

2008

3

19

1-3

10.3969/j.issn.1673-7555.2008.19.001

本文受湖南省卫生厅课题（项目编号：B2005-093 B2006-098）

本校为第一机构

目的 观察丙丁酚对高脂高胆固醇饲养的高脂血症小鼠体内C反应蛋白(CRP)活性及表达的影响,探讨丙丁酚的作用机制.方法 以高脂高胆固醇饲料饲喂小鼠,制作高脂血症模型.HE染色观察小鼠肝脏病变情况;采用相关检测试剂盒测定小鼠血脂水平、血浆中CRP和谷胱甘肽S转移酶的活性;采用逆转录聚合酶链式反应(RT-PCR)检测小鼠肝脏CRP mRNA的表达.结果 高脂高胆固醇饮食显著增加C57BL/6J和apoE-/-小鼠血脂水平(P

Objective To investigate the effects of probucol on high fat-high cholesterol（HFHC） dietinduced hyperlipidemia,and the activity and expression of C-reactive protein（CRP） in apo E knockout mice and genetic background C57BL/6J mice. Methods The mice models were build by fed HFHC-diet（ 15% lard and 0. 25% cholesterol）. The serum levels of lipids and the activity of CRP were determined by commercially provided kits. The liver slices were stained by hematoxylin eosin（HE） staining. The CRP mRNA expression levels in liver were demonstrated by ...