期刊论文

Effect of NaF and NaAsO2 on proliferation of rat hepatocyte

中文

中国现代医生

1673-9701

2012

50

7

1-3

10.3969/j.issn.1673-9701.2012.07.001

本校为第一机构

目的 研究氟化钠(NaF)和亚砷酸钠(NaAsO2)单独及联合作用对大鼠BRL-3A细胞增殖的影响.方法 设NaF( 1.0×10-5～1.0× 10-1)mol/L、NaAsO2( 1.0× 10-7～1.0× 10-3) mol/L不同浓度组(组间10倍差),分别作用于大鼠肝细胞,采用MTT法测定OD值,观察其对细胞增殖的影响.NaF在1.0× 10-3 mol/与NaAsO2在1.0×l0-6 mol/L、1.0×10-5 mol/L、1.0×10-4 mol/L浓度下,两者联合作用于大鼠肝细胞时,采用2×2的析因试验分析其对细胞增殖的影响.结果 氟、砷单独作用对肝细胞增殖的抑制率均随染毒剂量的增大而逐渐升高,而联合组在NaF为1.0×10-3 mol/L与NaAsO2为1.0×10-6 mol/L、1.0×10-5 mol/L、1.0×10-4 mo...

Objective To study the effect of NaF and NaAsO2 on proliferation of BRL-3A rat cells. Methods Rat hepatocytes were used to evaluate proliferating effects of NaF and NaAsO2 at concentrations of （1.0×10-5-1.0×10-1） mol/L and （1.0× 10-7-1.0×10-3） mol/L （10 times difference between two consecutive groups）, proliferation cell was observed by OD values measured by MTT assay. The combined effects of NaF at concentrations of 1.0×10-3 mol/L and NaAsO2 at 1.0×104 mol/L, 1.0×10-5 mol/L, 1.0×10-4 mol/L on proliferatiori of rat hepatocyte were...