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乳腺炎奶牛双重感染抗热分枝杆菌和象分枝杆菌的鉴定及药敏试验研究

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成果类型:
期刊论文
作者:
李文彬;纪凌云;徐冬蕾;刘海灿;赵秀芹;...
通讯作者:
Wan, K.
作者机构:
[李文彬; 吴移谋] Institution of Pathogenic Biology, Medical College, University of South China, Hengyang, 421001, China
[徐冬蕾; 赵秀芹; 刘海灿; 万康林] National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China
Department of Laboratory Medicine, Shanghai East Hospital, Tongji University, Shanghai, 200123, China
[纪凌云] National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, Department of Laboratory Medicine, Shanghai East Hospital, Tongji University, Shanghai, 200123, China
通讯机构:
[Wan, K.] N
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
语种:
中文
关键词:
非结核分枝杆菌;抗热分枝杆菌;象分枝杆菌;奶牛乳腺炎
关键词(英文):
Cow with mastitis;Mycobacterium elephantis;Mycobacterium thermoresistibile;Non-tuberculosis mycobacterium
期刊:
中华流行病学杂志
ISSN:
0254-6450
年:
2018
卷:
39
期:
5
页码:
669-672
基金类别:
传染病国家重点实验室项目(2014SKLID104); 国家重点基础研究发展计划(973计划)(2015CB554202); 国家科技重大专项(2013ZX10003002-001)~~;
机构署名:
本校为第一机构
院系归属:
医学院
摘要:
目的对疑似分枝杆菌感染乳腺炎奶牛进行病原及药敏谱调查。方法采集1头患乳腺炎奶牛的牛奶,采用4% NaOH预处理,接种于L-J培养基分离培养。阳性培养物利用抗酸染色和多位点PCR方法进行初步鉴定,采用16S rRNA、hsp65、ITS和SodA基因的多位点序列分析进行种的鉴定,利用Alamarblue显色法对分离菌株进行27种药物的药敏试验。结果从1头患乳腺炎奶牛的牛奶中同时分离获得2株抗酸染色阳性培养物,经PCR鉴定为非结核分枝杆菌,多位点序列分析鉴定为抗热分枝杆菌和象分枝杆菌。药敏试验表明这2株菌对利福平和异烟肼等大多数抗结核药物耐药,但对阿米卡星、莫西沙星、左氧氟沙星、乙胺丁醇、链霉素、妥布霉素、...
摘要(英文):
Objective: To understand the etiological characteristics and drug susceptibility of Mycobacterium thermoresistibile and Mycobacterium elephantis isolated from a cow with mastitis and provide evidence for the prevention and control of infectious mastitis in cows. Methods: The milk sample was collected from a cow with mastitis, which was pretreated with 4% NaOH and inoculated with L-J medium for Mycobacterium isolation. The positive cultures were initially identified by acid-fast staining and multi-loci PCR, then Mycobacterium species was identif...

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