Generation of INS-jGCaMP7f knock-in Ca2+ reporter human embryonic stem cell line, GZLe001-C, using CRISPR/Cas9-based gene targeting

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成果类型：

期刊论文

作者：

Liu, Xin;Zhang, Feng;Chen, Deqi;Yin, Jiaxiang;Bi, Zirong;...

通讯作者：

Guo, YY;Xu, Tao;Lin, Haopeng;Liu, HS

作者机构：

[Guo, Yanying; Liu, Xin] Peoples Hosp Xinjiang Uygur Autonomous Reg, Xinjiang Clin Res Ctr Diabet, Dept Endocrinol & Metab, Xinjiang Key Lab Cardiovasc Homeostasis & Regenera, Urumqi 830001, Peoples R China.

[Liu, HS; Lin, Haopeng; Yan, Jiawei; Yin, Jiaxiang; Chen, Lihua; Liu, Huisheng; Bi, Zirong; Xu, Tao; Dong, Qifei; Chen, Deqi; Liu, Xin; Peng, Wei; Zhang, Feng] Guangzhou Natl Lab, Guangzhou 510005, Peoples R China.

[Liu, HS; Lin, Haopeng; Yin, Jiaxiang; Chen, Lihua; Liu, Huisheng; Bi, Zirong; Xu, Tao] Guangzhou Med Univ, Sch Biomed Engn, Guangzhou 510180, Peoples R China.

[Liu, HS; Liu, Huisheng] Bioland Lab, Guangzhou 510005, Peoples R China.

[Dong, Qifei; Peng, Wei] Univ South China, Inst Cytol & Genet, Sch Basic Med Sci, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.

通讯机构：

[Liu, HS ; Lin, HP; Xu, T] G

[Guo, YY ] P

Peoples Hosp Xinjiang Uygur Autonomous Reg, Xinjiang Clin Res Ctr Diabet, Dept Endocrinol & Metab, Xinjiang Key Lab Cardiovasc Homeostasis & Regenera, Urumqi 830001, Peoples R China.

Guangzhou Natl Lab, Guangzhou 510005, Peoples R China.

Guangzhou Med Univ, Sch Biomed Engn, Guangzhou 510180, Peoples R China.

语种：

英文

关键词：

CRISPR/Cas9;Ca(2+);Insulin;hESC;jGCaMP7f

期刊：

Stem Cell Research

ISSN：

1873-5061

年：

2025

卷：

页码：

103633

DOI：

10.1016/j.scr.2024.103633

基金类别：

National Natural Science Foundation of China [32400682]; Natural Science Foundation of Xinjiang Uygur Autonomous Region [2022D01D51]; Guangdong Basic and Applied Basic Research Foundation [2023A1515010483]; Young Scientists Program of Guangzhou National Laboratory [QNPG23-02]; Young Scientists Program of Guangzhou Laboratory [QNPG23-10]; R & D Program of Guangzhou Laboratory [SRPG22-021]; National Key Research and Development Program of China [2021YFA1101300]

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摘要：

As a member of the single-fluorophore genetically encoded calcium indicators (GECIs), jGCaMP7f is widely applied to investigate intracellular Ca 2+ concentrations. Here, we established an INS-jGCaMP7f knock-in H1 human embryonic stem cell (hESC) line by integrating jGCaMP7f gene into insulin locus via CRISPR/Cas9 system. The reporter cell line not only effectively labelled the insulin-producing cells induced from hESC, but also reflected the cytosolic change of Ca 2+ level in response to different stimuli. This reporter cell line is a valuable research tool for studying functional maturation o...

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Generation of INS-jGCaMP7f knock-in Ca2+ reporter human embryonic stem cell line, GZLe001-C, using CRISPR/Cas9-based gene targeting

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