期刊论文

Liao, DF

英文

Biochemical and Biophysical Research Communications

0006-291X

2005

328

1

265-272

10.1016/j.bbrc.2004.12.159

The authors are indebted to Dr. Chen Yan in the University of Rochester for her critical discussions, suggestions, and valuable help in the preparation of the manuscript. This work was supported by the National Natural Science Foundation of China (30400265 and 30470719), the National Major Basic Research Program (973) of China (G2000056905) and the Natural Science Foundation of Hunan (03JJY3037).

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In the initial report, introducing a single phosphorothioate modification at the very 3′ terminus of the oligodeoxynucleotide primer has been shown to effectively protect the oligodeoxynucleotide degradation due to the 3′ exonuclease activity. In this study, we reported a novel finding that phosphorothioate modification at the 3′ end of primers could not only effectively prevent the primer from degradation, but could also mediate an off-switch extension by Pfu polymerase when primers also carry single or multiple mismatched bases located in ...