Advances in the engineering of the gene editing enzymes and the genomes: Understanding and handling the off-target effects of CRISPR/Cas9

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Yin, Yufang;Wang, Qian;Xiao, Li*;Wang, Fengjiao;Song, Zhuo;...

通讯作者：

Xiao, Li;Zhang, Jia

作者机构：

[Li, Kai; Liu, Xuan; Yin, Yufang; Zhou, Cuilan; Wang, Qian; Xing, Chungen; Xiao, Li] Soochow Univ, Affiliated Hosp 2, Lab Mol Med, Suzhou 215004, Peoples R China.

[Yin, Yufang] Southern Illinois Univ, Dept Neurosci & Pharmacol, Sch Med, Carbondale, IL 62702 USA.

[Wang, Fengjiao] Soochow Univ, Dept Pharm, Affiliated Childrens Hosp, Suzhou 215123, Peoples R China.

[Song, Zhuo] Genetalks Inc, Changsha 410013, Hunan, Peoples R China.

[He, Nongyue] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.

通讯机构：

[Xiao, Li] S

[Zhang, Jia] U

Soochow Univ, Affiliated Hosp 2, Lab Mol Med, Suzhou 215004, Peoples R China.

Univ South China, Chenzhou Peoples Hosp 1, Translat Med Inst, Natl & Local Joint Engn Lab High Through Mol Diag, Chenzhou 423000, Peoples R China.

语种：

英文

关键词：

Engineering;Gene editing;Gene therapy;Genome

期刊：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY

ISSN：

1550-7033

年：

2018

卷：

期：

页码：

456-476

DOI：

10.1166/jbn.2018.2537

基金类别：

Jiangsu Province's clinical medical science and technology program [BL2013016, QN-868]; Suzhou's Program [SZS201618, SYS201727]; Hunan Province's program [2016JJ2114, 2017SK2172]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81301267, 81372825]

机构署名：

本校为通讯机构

院系归属：

医学院

摘要：

In the past decades, significant progresses have been achieved in genetic engineering of nucleases. Among the genetically engineered nucleases, zinc finger nucleases, transcription activator-like (TAL) effector nucleases, and CRIPSPR/Cas9 system form a new field of gene editing. The gene editing efficiency or targeting effect and the off-target effect are the two major determinant factors in evaluating the usefulness of a new enzyme. Engineering strategies in improving these gene editing enzymes, particularly in minimizing their off-target effects, are the focus of this paper. Examples of usin...

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Advances in the engineering of the gene editing enzymes and the genomes: Understanding and handling the off-target effects of CRISPR/Cas9

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