期刊论文

中文

海洋科学

1000-3096

2003

27

4

28-34

10.3969/j.issn.1000-3096.2003.04.008

2001AA621140:国家高技术研究发展计划（863计划） 30170732:国家自然科学基金

本校为其他机构

26S蛋白酶体是真核生物中一种具有 ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程. 26S蛋白酶体由具有催化活性的 20S亚复合体和一个具有调节作用的 19S亚复合体组成,其中 19S亚复合体中的 ATP酶亚基是调节 26S蛋白酶体活性的重要组件.本文通过简并引物 PCR手段,从软体动物合浦珠母贝 (Pinctada fucata)中扩增到参与构成 19S亚复合体的 S4和 S7(MSS1)两个亚基的基因片段.这两个基因片段所编码的 ATP酶组件包含有 Gx4GKT, DEID, SAT和 H/QRxGRxxR等 26S蛋白酶体 ATP酶亚基的共同功能基序.这是首次在软体动...

The 26S proteasome is an eukaryotic ATP dependent protease complex that is responsible for selective degradation of abnomal proteins and naturally short lived proteins relate to metabolic regulation and cell cycle progression by ubiquitin pathway so as to regulate a variety of cellular activities. The complex is composed of two subcomplexes, the proteolytic core 20S particle and the 19S regulatory particle. Based on the sequences of amino acids and its corresponding DNA of 26S proteasomal ATPase subunits from human and rat, two degenerate primers were designed and synthesized, and two cDNA fra...