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Resorcinol monophosphate ester as a new substrate for fluorometric detection of alkaline phosphatase activity in milk products

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成果类型:
期刊论文
作者:
Li, Hongchen;Huang, Ning;Cheng, Jing;Ouyang, Min;Sun, Jingbo;...
通讯作者:
Xu, D
作者机构:
[Li, Hongchen; Xu, D; Peng, Chenzhan; Xu, Dong; Ouyang, Min; Sun, Jingbo] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & By Prod Further Proc, Changsha 410004, Peoples R China.
[Huang, Ning] Tianjin Univ, Inst Med Engn & Translat Med, Tianjin 300072, Peoples R China.
[Cheng, Jing] Hunan Acad Inspection & Quarantine, Technol Ctr Changsha Customs, Changsha 410004, Peoples R China.
[Cao, Xuan] Univ South China, Dept Pharm, Henyang 421001, Peoples R China.
通讯机构:
[Xu, D ] C
Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & By Prod Further Proc, Changsha 410004, Peoples R China.
语种:
英文
关键词:
Alkaline phosphatase;Activity;Fluorescence method;Pasteurization;Phosphate ester
期刊:
Microchemical Journal
ISSN:
0026-265X
年:
2023
卷:
191
页码:
108878
基金类别:
CRediT authorship contribution statement Hongchen Li: Methodology, Investigation, Writing – original draft. Ning Huang: Methodology, Investigation, Validation, Writing – review & editing, acquisition. Jing Cheng: Methodology, Validation, Writing – review & editing. Min Ouyang: Methodology, Validation, Writing – original draft, Validation. Jingbo Sun: Methodology, Writing – original draft, Validation. Chenzhan Peng: Methodology, Writing – review & editing, Project administration. Xuan Cao: Methodology, Writing – review & Acknowledgements This work was supported by funding from the National Natural Science Foundation of China (21505162), the Grain-Oil Process and Quality Control 2011 Collaborative and Innovative Grant from Hunan province, Natural Science Foundation of Hunan Province, China (2020RC3042, 2022SK2102), Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning (2020KFJJ05), and Hunan Postgraduate Science and Technology Innovation Fund Project (CX202102068).
机构署名:
本校为其他机构
院系归属:
药学与生物科学学院
摘要:
Alkaline phosphatase (ALP) is an indicator of sterilization efficacy of milk products and its activity monitoring is essential for ensuring food safety and controlling product quality. Here, a novel fluorometric method for the determination of ALP activity based on resorcinol monophosphate ester (RME) as a new substrate was proposed to verify pasteurization of milk products. RME was cheap and was simply and novelly synthesized by a two-step method using resorcinol (RC) as the raw material. The mechanism is, after dephosphorylation, RME was transformed to RC, which subsequently react with dopam...

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