期刊论文

Du, Ke-Jie;Lin, YW

英文

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

1570-9639

2023

1871

3

140897

10.1016/j.bbapap.2023.140897

CRediT authorship contribution statement Yu Feng: Data curation, Writing – original draft. Yao Dong: Data curation, Writing – original draft. Ke-Jie Du: Formal analysis, Validation, acquisition. Xi-Chun Liu: Formal analysis, Validation. Shu-Qin Gao: Visualization. Ying-Wu Lin: acquisition, Supervision, Writing – review & editing.

本校为第一且通讯机构

Natural and artificial nucleases have extensive applications in biotechnology and biomedicine. The exploration of protein with potential DNA cleavage activity also inspires the design of artificial nuclease and helps to understand the physiological process of DNA damage. In this study, we engineered four human cytochrome c (Cyt c) mutants (N52S, N52A, I81N, and I81D Cyt c), which showed enhanced DNA cleavage activity and degradation in comparison with WT Cyt c, especially under acidic conditions. The mechanism assays revealed that the superoxide (O2 & BULL; ) plays an important role in...