期刊论文、会议论文

Lin, Ying-Wu

英文

Journal of Molecular Catalysis B: Enzymatic

1381-1177

2016

134

367-371

8th Meeting on OxiZymes

JUL 03-06, 2016

Wageningen, NETHERLANDS

[Wu, Lei-Bin;Du, Ke-Jie;Nie, Chang-Ming;Lin, Ying-Wu] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.^[Gao, Shu-Qin;Wen, Ge-Bo;Lin, Ying-Wu] Univ South China, Lab Prot Struct & Funct, Hengyang 421001, Peoples R China.^[Tan, Xiangshi] Fudan Univ, Dept Chem, Shanghai Key Lab Chem Biol Prot Res, Shanghai 200433, Peoples R China.^[Tan, Xiangshi] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China.

COST Act Syst Biocatalysis

PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS

ELSEVIER SCIENCE BV

10.1016/j.molcatb.2016.08.018

本校为第一且通讯机构

To reveal the structure-function relationship of heme proteins, and to provide clues for creating artificial heme proteins with improved functions, we here use myoglobin (Mb) as a model protein, and report that its peroxidase activity can be enhanced by construction of two distal histidines and a channel to the heme pocket. It showed that in addition to a single distal histidine with a suitable distance to the heme iron (Phe43 to His43 mutation), a second distal histidine (Leu29 to His29 mutation) can work cooperatively to increase the turnover number, mimicking the role of well-known His-Arg ...