期刊论文

Colorimetric detection of Hg~(2+) with bromelain-AuNCs peroxidase-like activity

中文

应用化工

1671-3206

2020

49

5

1319-1324

10.16581/j.cnki.issn1671-3206.2020.05.018

国家自然科学基金（81473021）； 衡阳市重点实验室项目资助（2018KJ110）；

本校为第一机构

制备了具有模拟过氧化物酶活性的菠萝蛋白酶-金纳米簇,可催化3,3',5,5'-四甲基联苯胺(TMB)-H_2O_2体系显色。因此,构建了Bromelain-AuNCs-TMB-H_2O_2-Hg~(2+)比色传感平台,并对该体系的催化显色反应机制进行了研究。本方法检测Hg~(2+)的线性范围为6.25 nmol/L ~ 3.5 μmol/L,检出限为4.3 nmol/L。本方法成本低,简便可行,选择性好,为环境水样中的Hg~(2+)检测提供了一种新方法。

Bromelain-gold nanoclusters(AuNCs)was successfully prepared that possess simulated peroxidase activity,it catalyzes the color development of the 3,3 ',5,5 '-tetramethylbenzidine(TMB)-H_2O_2 system.Therefore,Bromelain-AuNCs-TMB-H_2O_2-Hg~(2+) colorimetric sensing platform was designed and researched the catalytic color reaction mechanism in the system.The linear range of detection of Hg~(2+) extends from 6.25 nmol/L ~ 3.5 μmol/L,and the lower detection limit is 4.3 nmol/L.The method was cost-effective,simple and feasible,with high selectivity,thus serving a...