期刊论文

中文

应用化工

1671-3206

2018

47

3

611-613

10.16581/j.cnki.issn1671-3206.20171221.006

国家自然基金青年基金项目支持（11205085）； 湖南省教育厅优秀青年项目资助（15B202）；

本校为第一机构

在pH 7.0的磷酸盐缓冲液中,葡萄糖氧化酶(GOx)催化葡萄糖与氧反应生成H_2O_2,在H_2SO_4介质中H_2O_2与I~-发生氧化还原反应生成I_2,过量的I~-与I_2生成I_3~-,I_3~-使阳离子荧光剂罗丹明6G位于553 nm波长处的荧光发生猝灭,猝灭程度与葡萄糖浓度相关,据此建立检测葡萄糖的新方法.方法线性范围为2.0 ×10~(-7) ~1.4 ×10~(-5) mol /L,检出限为6.77 ×10~(-8) mol /L.将该方法用于血糖样品测定,加标回收率为85.0% ~102.5%,操作简便、结果准确可靠.

In phosphate buffer solution(pH 7.0),glucose oxidase(GOx)can catalyze the reaction of glucose with oxygen generate hydrogen peroxide, then hydrogen peroxide reaction with excess I~- in dilute sulfuric acid solution to form I_3~-. The I_3~- combines with Rhodamine 6G(Rh6G)to form Rh6G-I3 associated particles which result in fluorescence quenching at 553 nm. Based on the significant quenching effect of glucose on the fluorescence of Rh6G,a new method with high sensitivity and good selectivity for the determination of glucose was established. Unde...