期刊论文

A magnetoelastic sensor method for arsenic based on aptamer recognition activity

中文

应用化工

1671-3206

2018

47

12

2800-2805

10.16581/j.cnki.issn1671-3206.20181013.012

湖南省教育厅重点课题（14A123）； 湖南省科技厅科技计划重点课题（2015SK2001）；

本校为第一机构

使用三价砷(As(III))的核酸适配体Ars-3为识别元件,利用金纳米(AuNPs)和氧化石墨烯(GO)的信号放大作用,建立一种新的无线传感方法检测痕量砷.通过设计一条标记巯基的ssDNA,与Ars-3互补形成一条不完全的dsDNA,在合适的条件下dsDNA能在巯基部位标记AuNPs.当加入As(III)时,由于As(III)能与dsDNA中的Ars-3特异性的结合,形成[As(III) -Ars-3]配合物,并游离出ssDNA,又因GO对ssDNA有很强吸附作用,而被磁弹片表面的GO吸附而沉积,导致磁弹片振动频率的改变(Δf), 据此可实现As(III)的定量检测.优化后的检测条件为:将终浓度0.5 μmol /L的dsDNA-AuNPs加入小玻璃管中,依次加入磁弹片、不同体积的砷标液、2%...

The arsenic (III) aptamer (Ars-3) was used as a recognition element, the signal amplification of gold nanoparticles (AuNPs) and graphene oxide(GO) was used to establish a new wireless sensor method for the detection of trace arsenic (III). A short SH-labled ssDNA has been designed,which could hybridize Ars-3 aptamer to form a partial dsDNA. GO has strong affinity for ssDNA. On adding As (III), the Ars-3 aptamers is separated firstly from dsDNA probe due to the formation of aptamer-As (III) complex, thus the short SH-labled ssDNA can be captured...