期刊论文

A dual phase extraction combined with HPLC method was developed to detect Flos Lonicerae flavonoids in cell culture medium

中文

应用化工

1671-3206

2018

47

2

410-412,415

10.16581/j.cnki.issn1671-3206.2018.02.025

15B211:湖南省教育厅优秀青年项目 2015226:湖南省大学生研究性学习和创新性实验计划

本校为第一机构

乙醇-磷酸氢二钾双水相体系萃取细胞培养液中的山银花黄酮类化合物,用高效液相色谱法分析样品中的山银花黄酮类化合物的含量,色谱柱为C_(18),流动相为甲醇-水(50% ∶ 50% v /v,用磷酸酸化至pH 4.0),流速1.0 mL/min,检测波长352 nm。结果表明,双水相萃取山银花黄酮上相乙醇,得到5个信号较强的化合物,其色谱行为与未经萃取的相同浓度山银花黄酮粗提物样品基本一致;萃取山银花黄酮粗提物细胞培养液样品获同样结果,表明乙醇-磷酸氢二钾双水相萃取结合液相色谱可检测细胞培养液中的山银花粗提物的黄酮化合物,该双水相能较好萃取并浓缩黄酮类化合物分配于上相乙醇中,有利于成分复杂、低浓度生物样品中...

The Flos Lonicerae flavonoids in cell culture mediumin was extracted by the ethanol-K_2HPO_4 two-phase conditions and its content was detected by HPLC with a C_(18) column. Samples were eluted using methanol /water (50%∶ 50% v /v) with phosphoric acid (apparent pH 4.0) at a flow rate of 1.0 mL /min and detected at 352 nm. Results showed that,with the two-phase system extracting flavonoids crude extract from Flos Lonicerae,chromatographic behavior of the 5 obvious signals from the upper phase sample were almost the same as the flavonoids crude ...