期刊论文

中文

应用化工

1671-3206

2018

47

3

627-630

10.16581/j.cnki.issn1671-3206.2018.03.034

湖南省教育厅重点课题（14A123）； 湖南省科技厅科技计划重点课题（2015SK2001）；

本校为第一机构

镉特异性功能化核酸适配体(FA_(Cd) )在无镉体系中能阻碍金纳米(GNPs)与罗丹明6G(Rh6G)聚集,体系共振光散射强度较低.当体系中存在镉离子时,FA_(Cd)与之特异性结合,从RNA位点处断裂产生适配体片段(FAF), FAF与GNPs和Rh6G形成GNPs-FAF-Rh6G聚合物,共振光散射强度大幅增加,共振光散射强度值(I_(RLS) )与镉的浓度(c_(Cd)~(2 +) )在0.28 ~ 11.2 ng /mL范围内呈现良好线性关系,检出限为0.04 ng /mL,相对标准偏差≤5%,加标回收率为96.4% ~ 101.4%.

Cadmium specific functional aptamer(FA_(Cd) )can hinder gold nanoparticles and Rhodamine 6G from gathering in a system without cadmium,keep system's resonant light scattering at a low level. When cadmium exist in system, it can specific binding with FA_(Cd) and activating enzyme activity of FA_(Cd),FA_(Cd) break from the RNA site, fragments (FAF)form large size Rh6G-FAF-GNPs polymer with Rh6G and GNPs, resulting a greatly increase of system's resonant light scattering. RLS has a good linear relationship with cadmium ion concentration in range of 0.28 ~ 11.2 ng /mL, limit of detection is 0.04 n...