To study the best extraction conditions of flavonids from Pinus massoniana needles in a invariable thermal situation,using ethanol as a solvent. The influence of ethanol concentration, liquid to material ratio,extraction time,extraction temperature on flavonids from Pinus massoniana were investigated. The results showed that the best extraction conditions concluded from single factor experiments and orthogonal test were ethanol concentration was 55%, the solid-liquid ratio was 1∶ 55 g/mL, the extraction time was 60 min, the extraction temperat...