期刊论文

中文

应用化工

1671-3206

2017

46

4

798-800,803

10.16581/j.cnki.issn1671-3206.20170222.024

湖南省大学生研究性学习和创新性实验计划项目（2015226）； 南华大学分子靶标新药研究协同创新中心大学生/研究生创新性实验计划项目；

本校为第一机构

建立同时测定黄豆中黄豆苷、染料木苷、染料木素的RP-HPLC法,为豆类食品中以上有效化学成分的检测和合理食用提供参考。样品经80%甲醇超声提取15 min,采用Eclipse XDB-C_(18)( 4. 6 mm × 250 mm,5 . 0 μm)色谱柱,以甲醇∶ 0. 1%磷酸= 62. 5∶ 37. 5为流动相,流速为1. 00 mL/min,进样量为10 μL,检测波长为254 nm。以峰面积计算结果为黄豆苷、染料木苷、染料木素分别在0. 5 ~ 32. 0 mg /L,4 . 0 ~ 128. 0 mg /L,0. 1 ~ 16. 0 mg /L呈良好线性关系,相关系数r均为0. 999 9;测定黄豆苷、染料木苷、染料木素的高、中、低浓度精密度均< 2. 0%,三成分的重现性精密度分别为1. 67%,0. 06%,0. 07%,表明该...

To establish a RP-HPLC method for the simultaneous determination of daidzein,genistin, genistein in soybean, soybean milk and bean dreg,which provide a reference for beans above the active chemical components of detection and reasonable use. After the samples were extracted with 80% methanol by ultrasonic treatment for 15 min, they were analysed by RP-HPLC with Eclipse XDB-C_(18)( 4. 6 mm × 250 mm,5 . 0 μm) column under methanol∶ 0. 1% phosphoric acid = 62. 5∶ 37. 5 as the mobile phase at a flow rate of 1. 00 mL /min. The injection volume w...