期刊论文

中文

应用化工

1671-3206

2014

43

5

957-961

10.16581/j.cnki.issn1671-3206.2014.05.010

YZ201013:江苏省预防医学科研课题指导性项目 CS20102018:常州市科技局项目

本校为第一机构

建立了用反相高效液相色谱-紫外检测法同时检测人尿中6种多环芳烃羟基代谢产物的新方法。采用 Dia-monsil TM C18（5μm，150 nm ×4.6 nm）色谱柱分离，以 V（甲醇）： V（乙酸钠缓冲液）=66：34为流动相，在波长280 nm检测，流速为0.95 mL/ min。结果表明，当9-羟基菲、α-萘酚、β-萘酚、1-羟基芘、2-羟基芴和9-羟基芴的摩尔浓度分别为0.105~500.0μmol/ L，0.106~135.0μmol/ L，0.120~135.0μmol/ L，0.085~50.00μmol/ L，0.094~200.0μmol/ L 和0.101~200.0μmol/ L 时，峰面积与质量浓度呈很好的线性关系，相对标准偏差分别为2.5％~4.4％，2.1％~4.3％，2.3％~3.2％，1.7％~3.9％，1.9％...

A novel method for assaying major metabolites of 6 kinds of polycyclic aromatic hydrocarbons in human urine by RP-HPLC-UV were established. These were detected at 280 nm and separated on Dia-monsil TM C18(5 μm,150 nm × 4. 6 nm)by using sodium acetate buffer solution(66: 34,V: V)as mobile phase when the flow rate was 0. 95 mL/ min. The result showed that the calibration curves of 9-hydroxy-phenanthrene,α-naphthol,β-naphthol,1-hydroxypyrene,2-hydroxyfluorene and 9-hydroxyfluorene showed good linearities in the ranges of 0. 105 ~ 500. 0 μmol/...