期刊论文

中文

应用化工

1671-3206

2013

42

1

161-165

10.16581/j.cnki.issn1671-3206.2013.01.001

湖南省科学技术计划立项项目（2010SK3039）；

本校为第一机构

利用荧光同步扫描和双波长法两种技术的结合,建立一种新的荧光分析法,对牛奶中的诺氟沙星(NFLX)和氧氟沙星(OFLO)残留进行检测。结果表明,NFLX的线性范围为10~600 μg/L,检出限为0.29 μg/L;OFLO的线性范围为20~1 200 μg/L,检出限为0.77 μg/L。对高、中、低三组浓度的混合标准溶液精密度实验,结果表明, NFLX的相对标准偏差小于1.41%,样品的加标回收率为95.9%~101.0%。OFLO的相对标准偏差小于2.51%,样品的加标回收率为96.3%~100.2%。本方法具有方法简便、准确度和精密度高等优点。

Based on the combination of synchronous scanning fluorescence and dual-wavelength method, a new fluorescence method was established for simultaneous determination of norfloxacin （NFLX） and ofloxacin （OFLO） residual in milk. The results showed that the linearity range of NFLX was 10 - 600 μg/L, detection limit was 0.29μg/L;and the linearity range of OFLO was 20 - 1 200 μg/L, detec- tion limit was 0.77 μg/L. The result of precision test for high, medium and low concentration indicated that the relative standard deviation （RSD） of NFLX a...