期刊论文

中文

应用化工

1671-3206

2013

42

8

1532-1537

10.16581/j.cnki.issn1671-3206.2013.08.005

江苏省预防医学科研课题指导性项目（YZ201013）； 常州市科技局课题（CS20102018）；

本校为第一机构

应用三维荧光法建立了直接测定人尿中的1-羟基芘、β-萘酚、9-羟基菲的新方法。通过β-环糊精增敏,三维荧光扫描选择测量波长。结果表明,在波长对λex/λem=275/385 nm时测定1-羟基芘,其线性范围和检出限分别为0.007～8.00μmol/L和2.30 nmol/L;在波长对为λex/λem=325/350 nm时测定β-萘酚,其线性范围和检出限分别为0.049～50.0μmol/L和12.5 nmol/L;在波长对为λex/λem=260/475 nm时测定9-羟基菲,其线性范围和检出限分别为0.023～40.0μmol/L和7.90 nmol/L。该方法已成功用于人尿中痕量1-羟基芘、β-萘酚、9-羟基菲的测定,与高效液相色谱法比较,结果满意,同时对β-环糊精增敏机制进行了初步...

A novel method for direct determination of 1-hydroxypyrene（1-OHP）,β-naphthol（β-NAP） and 9-hydroxyphenanthrene（9-OHPe） in human urine was performed using their three-dimensional fluorescence phenomenon,through β-cyclodextrin enhancing fluorescence and choosing wavelength of determination by three-dimensional fluorescence scan.Results showed that the linear ranges of 1-OHP,β-NAP and 9-OHPe were 0.007～8.00 μmol/L at λex/λem=275/385 nm,0.049～50.0 μmol/L at λex/λem=325/350 nm,and 0.023～40.0 μmol/L at λex/λem=260/475 nm,respecti...