期刊论文

中文

暨南大学学报(自然科学与医学版)

1000-9965

2017

38

1

25-29

10.11778/j.jdxb.2017.01.004

国家自然科学基金项目（81372579）； 湖南省卫生厅医药卫生资助科研项目（B2014-048）；

本校为第一机构

目的:探讨 miR-503通过调控 Bcl-2的表达介导肝癌耐药细胞株 BEL-7402 /5-FU对5-氟尿嘧啶敏感性的影响.方法: microRNA(miRNA)芯片筛选 BEL-7402与BEL-7402 /5-FU细胞中表达差异的miR-503为候选的miR- NA;脂质体转染法将miR-503模拟物瞬时转染至BEL-7402 /5-FU细胞;实时定量聚合酶链式反应(qRT-PCR)检测 BEL-7402 /5-FU及其亲本细胞中 miR-503、Bcl-2 mRNA的表达水平;Western Blot观察肝癌细胞中 Bcl-2蛋白的表达水平;CCK-8法检测细胞药物敏感性的改变.结果:相比于BEL-7402细胞,miR-503在BEL-7402 /5-FU细胞中表达水平下调,而 Bcl-2蛋白的表达水平上调;miR-503过表达后BEL-7402 /5-FU细胞中的Bc...

Aim:This study is to investigate the effects of miR-503 on 5-fluorouracil sensitivity of liver cancer drug resistant cell lines BEL-7402 /5-FU by down-regulating Bcl-2 expression. Methods: Differentially expressed miR-503 between BEL-402 and BEL-7402 /5-FU cells was screened by microRNA (miR- NA),and was to be a candidate miRNA. MiR-503 mimic matter was transient transfected to the BEL- 7402 /5-FU cells by liposome transfection method. MiR-503 and Bcl-2 mRNA expression level of BEL- 7402 /5-FU and its parental cells were measured by qRT-PCR. Bcl-2 protein level was detected by west- ern blot. ...