期刊论文

HPA-siRNA Promotes Apoptosis and Inhibits Proliferation, Invasion and Migration of Skin Squamous Cell Carcinoma A431 Cells

中文

中国皮肤性病学杂志

1001-7089

2019

33

9

996-1000

10.13735/j.cjdv.1001-7089.201812145

本校为第一机构

目的探讨乙酰肝素酶(HPA)小干扰RNA(siRNA)对皮肤鳞状细胞癌A431细胞增殖、凋亡、侵袭和迁移的影响及其机制。方法将体外培养的A431细胞分为空白对照组(未转染) 、阴性对照组(转染NC-siRNA)和HPA沉默组(转染HPA-siRNA),RT-PCR检测各组细胞中HPA mRNA的表达,MTT法、流式细胞仪和Transwell分别检测细胞增殖、凋亡、侵袭和迁移; Western blot检测细胞中HPA蛋白和PI3K/AKT信号通路相关蛋白PCNA、MMP-2、Bcl-2、p-AKT和AKT的表达。结果与空白对照组相比,HPA沉默组细胞在2 ~ 3 d生长过程中的A值明显下降,侵袭和迁移细胞数均明显减少,HPA mRNA和HPA、PCNA、MMP-2、Bcl-2、p-AKT蛋白的表达水平均明显降低...

Objective To investigate the effect and mechanism of small interfering RNA (siRNA) of heparanase (HPA) on proliferation,apoptosis, invasion and migration of cutaneous squamous cell carcinoma A431 cells. Methods A431 cells were divided into control group (untransfected) ,negative control group (transfected with NCsiRNA) and HPA silent group (transfected with HPA-siRNA). The expression of HPA mRNA was detected by RT-PCR,the proliferation,apoptosis,invasion and migration of cells were detected by MTT,flow cytometry and transwell respectively. The expression of HPA protein and PI3K/AKT signaling p...