期刊论文

Relationship between Polymorphism of CYP17 Gene and Syndrome Differentiation Typing Based on Traditional Chinese Medi- cine in Acne

中文

中国皮肤性病学杂志

1001-7089

2008

22

9

559-561

湖南省卫生厅科研基金（编号：2006-238）；

本校为第一机构

目的 探讨CYP17基因多态性与痤疮中医分型的相关性.方法 提取肝郁气滞型、湿热内蕴型痤疮患者和正常对照者的血DNA标本.设计引物通过PCR技术扩增出包括CYP17基因多态位点的片段,用限制性内切酶Ms-pA1 Ⅰ进行酶切,产物在2%琼脂糖凝胶上电泳,确定出CYP17基因的3种基因型(A1A1、A1A2、A2A2),并经测序证实.结果 肝郁气滞型组基因型A1A2频率、湿热内蕴型组基因型A1A2频率间及分别与正常对照组基因型A1A2频率比较,差异无显著性意义(P＞0.05);肝郁气滞型组基因型A2A2频率和正常对照组基因型A2A2频率比较,差异无显著性意义(P＞0.05);湿热内蕴型组基因型A2A2频率分别与肝郁气滞型组基因型A2A2频率和正常对...

Objective To study the relationship between CYP17 gene and syndrome differentiation typing based on Traditional Chinese Medicine（TCM）patients with acne. Methods The cases study including the patients with Liver-qi and damp-heat in the in- terior and the controls was conducted. The polymorphism was investigated by PCR using DNA from peripheral blood lymphocytes. The transition （T--~C） in the risk allele （A2） produced a new recognition site for the restriction enzyme MspA1. Three genotypes of CYP17 gene（ A1A1, A1A2, A2A2）were determined a...