期刊论文

中文

中国皮肤性病学杂志

1001-7089

2001

15

1

4-6,34

10.3969/j.issn.1001-7089.2001.01.003

湖南省科委重点课题资助项目；

本校为第一机构

目的　了解泌尿生殖道支原体的耐药性机理 ,指导临床治疗。方法　采用微量肉汤稀释法测定 2 81株UU对 5种抗菌药物的最低抑菌浓度 （MIC） ,用PCR法扩增tetM基因并酶切 ,用UUMB抗原基因引物对tetM基因阳性UU株进行生物群的研究。结果　 2 81株UU中 82株对四环素MIC为 16～ 2 5 6μg/ml ,3 9株为 8μg/ml ,7株为 4μg/ml,15 3 株MIC

Objective To monitor resistance of urogenital mycoplasma periodically, and to instruct rational application of antibiotics clinically. Methods The minimal inhibitory concentrations(MICs) of 281 strains UU to 5 kinds of antimicrobial a gents were determined using microbroth dilution assay, the tetM gene in the isolates was detected by PCR and the PCR products were digested by Hpa Ⅱ restrictive endonuclease. PCR analysis was used to sutdy the biotypes of UU with tetM gene with primers derived from MB antigen gene. Results Of 281 strains U...