摘要：
This study aimed to evaluate the biological effects of gamma irradiation on zebrafish embryos. Different doses of gamma rays (0.01, 0.05, 0.1, 0.5 and 1 Gy) were used to irradiate zebrafish embryos at three developmental stages (stage 1, 6 h post-fertilization (hpf); stage 2, 12 hpf; stage three, 24 hpf), respectively. The survival, malformation and hatching rates of the zebrafish embryos were measured at the morphological endpoint of 96 hpf. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione S-transferase (GST) were assayed. Morphology analysis showed that gamma irradiation inhibited hatching and induced developmental toxicity in a dose-dependent manner. Interestingly, after irradiation the malformation rate changed not only in a dose-dependent manner but also in a developmental stage-dependent manner, indicating that the zebrafish embryos at stage 1 were more sensitive to gamma rays than those at other stages. Biochemical analysis showed that gamma irradiation modulated the activities of antioxidant enzymes in a dose-dependent manner. A linear relationship was found between GPx activity and irradiation dose in 0.1–1 Gy group, and GPx was a suitable biomarker for gamma irradiation in the dose range from 0.1 to 1 Gy. Furthermore, the activities of SOD, CAT, GR and GPx of the zebrafish embryos at stage 3 were found to be much higher than those at other stages, indicating that the zebrafish embryos at stage 3 had a greater ability to protect against gamma rays than those at other stages, and thus the activities of antioxidant enzymes changed in a developmental stage-dependent manner.
作者机构：
[丁德馨; 石建军] School of Resources and Safety Engineering, Central South University, Changsha, 410083, China;[张志恒; 丁德馨; 栗建新; 石建军; 刘华良; 刘权] School of Urban Construction, University of South China, Hengyang, 421001, China
通讯机构：
School of Resources and Safety Engineering, Central South University, Changsha, China
作者机构：
[杨雨山; 喻清; 丁德馨] School of Resources and Safety Engineering, Central South University, Changsha, 410083, China;[王浩; 李峰; 喻清; 丁德馨; 杨雨山; 胡南] Key Discipline Laboratory of Defense Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, 421001, China
通讯机构：
School of Resources and Safety Engineering, Central South University, Changsha, China
摘要：
A kind of functional plant composite adsorbent was prepared through diethylenetriamine modifing the Cycas revoluta leaves for low concentration of U(VI) in waste water. The dynamic behavior of the adsorption, the Langmuir and Freundlich equations and the thermodynamic parameters was were also examined in the present study. The experimental results showed that diethylenetriamine functionalized Cycas revoluta leaves used for low concentration of uranium possessed many advantages such as widely raw material sources, simple and economy for preparation, less adsorbent dosage, short balance time, high adsorption efficiency, good chemical and mechanical stability, and can be effective regeneration or reused.
作者机构：
[喻清; 丁德馨] School of Resources and Safety Engineering, Central South University, Changsha, 410083, China;[余园平; 罗艺; 胡南; 王启方; 喻清; 李登科; 丁德馨] Key Discipline Laboratory of Defense Biotechnology in Uranium Mining and Hydrometallurgy, University of South china, Hengyang, 421001, China
通讯机构：
School of Resources and Safety Engineering, Central South University, Changsha, China
作者机构：
[董发勤; 刘明学; 聂小琴] Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Southwest University of Science and Technology, Mianyang, China;[张东] Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang, China;[刘宁; 聂小琴] Key Laboratory of Radiation Physics and Technology, Institute of Nuclear Science and Technology, Sichuan University, Chengdu, China;[丁德馨; 李广悦; 聂小琴] Fundamental Science on Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, China
通讯机构：
Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Southwest University of Science and Technology, Mianyang, China
作者机构：
[王晓亮; 彭国文] School of Chemistry and Chemical Engineering, University of South China, Hengyang, China;[孙世雄] Qinghai Jinrui Mining Development Co., Ltd., Xining, China;[肖方竹; 丁德馨; 彭国文; 胡南; 张志军] Key Discipline Laboratory for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, China
作者机构：
[刘明学; 聂小琴; 董发勤] Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Southwest of Science and Technology, Mianyang, China;[张东] Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang, China;[Liu, Ning] Key Laboratory of Radiation Physics and Technology, Institute of Nuclear Science and Technology, Sichuan University, Chengdu, China;[丁德馨; 刘宁] Fundamental Science on Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, China
通讯机构：
Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Southwest of Science and Technology, Mianyang, China
摘要：
A defined mixed bioleaching consortium (constructed by Acidithiobacills ferrooxidans ATCC 23270, Leptospirillum ferriphilum YSK, Sulfobacillus thermosulfidooxidans ST, Acidithiobacills thiooxidans A01 and Acidithiobacills caldus S1) was used to investigate the fluoride stress response. The results showed that its growth and iron oxidation rate were obviously inhibited, while the sulphur oxidation was only barely restrained. Furthermore, the community dynamics and gene expression were assayed respectively by using real-time quantitative polymerase chain reaction assay (RT-PCR) and functional gene arrays (FGAs). From the analysis of community dynamics, the most obviously inhibited strains were S. thermosulfidooxidans ST, while L. ferriphilum YSK still maintained stable growth. After 4.8 mM of fluoride stress for 28 h, the S. thermosulfidooxidans cell concentration was decreased by about 517.8 times more than in non-stress state. From the analysis of gene expression, fluoride stress was seen to exert pleiotropic effects on different species in the consortium. The dominant species played the pivotal role in resisting fluoride stress and maintaining activities in the system, yet the inferior species also had a critical function of assisting the survival of the dominant species.
作者机构：
[董发勤; 刘明学; 聂小琴] Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Southwest University of Science and Technology, Mianyang, China;[张东] Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang, China;[刘宁] Key Laboratory of Radiation Physics and Technology, Institute of Nuclear Science and Technology, Sichuan University, Chengdu, China;[丁德馨; 李广悦] Fundamental Science on Biotechnology in Uranium Mining and Hydrometallurgy Laboratory, University of South China, Hengyang, China
通讯机构：
Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Southwest University of Science and Technology, Mianyang, China
作者机构：
[李峰; 陈伟; 丁德馨] School of Nuclear Resource Engineering, University of South China, Hengyang, China;[李峰; 陈伟; 胡南; 丁德馨] Key Discipline Laboratory for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, China
通讯机构：
School of Nuclear Resource Engineering, University of South China, Hengyang, China
关键词：
含金硫精矿;金;微波焙烧;预处理;浸出率;表面形貌
摘要：
对难浸含金硫精矿进行微波焙烧,考察微波功率、矿量（即样品质量）和焙烧时间对样品质量损失率和浸出率的影响。结果表明:当微波功率为16 k W、焙烧时间为50 min、矿量为900 g时,样品质量损失率可达28.12%,浸出率可达71.56%,较原矿直接碘化浸出率（9.82%）有了大幅提高。利用XRD和SEM技术分析焙烧前后样品的成分和表面形貌,微波焙烧后的样品较原矿更为松散、多孔,更利于浸出。
