摘要：
Neisseria meningitidis can cause severe and fulminant diseases such as meningitis. Meningococcal capsular polysaccharide (PS) is a key virulence determinant that is not able to induce immunological memory. Conjugation of PS to a carrier protein can significantly increase the immunogenicity of PS and induce immunological memory. Due to the classically described carrier-induced epitopic suppression (CIES) mechanisms, a strong immune response against the carrier protein could suppress the immune response to PS after coadministration of free carrier protein with the conjugate vaccine. However, it was not clear whether suppressing Or enhancing the protein-specific immunogenicity could improve the PS-specific immunogenicity of the conjugate vaccine. Thus, moderate PEGylation, extensive PEGylation and oligomerization were used to regulate the immunogenicity of tetanus toxoid (TT) in the conjugate vaccine (PS-TT). Moderate PEGylation led to a 2.7-fold increase in the PS-specific IgG titers elicited by PS-IT. In contrast, extensive PEGylation and oligomerization of TT led to 1.4-fold and 1.6-fold decrease in the PS-specific IgG titers elicited by PS-TT, respectively. The PS-specific immunogenicity of PS-TT can be increased by moderate PEGylation through mild suppression of the TT-specific immunogenicity. The PS-specific immunogenicity of PS-TT was decreased through significant suppression or enhancement of the TT-specific immunogenicity. Thus, our study contributes to understand the CIES mechanisms and improve the PS-specific immunogenicity of a meningococcal PS conjugate vaccine. (C) 2015 Elsevier Ltd. All rights reserved.
期刊：
Journal of Radioanalytical and Nuclear Chemistry,2013年298(2):903-908 ISSN：0236-5731
通讯作者：
Wang, Yanfei
作者机构：
[Yang, Yan; Wang, Yanfei] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Feng, Yixiao; Shi, Weiqun; Wang, Lin] Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China.
通讯机构：
[Wang, Yanfei] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Uranium;BSA;Secondary structure;FT-IR/ATR
摘要：
Interest in bio-toxicology of uranium resulting from its radioactive heavy metal property has been growing enormously in recent years. The interactions between uranium(VI) [U(VI)] and bovine serum albumin (BSA) at physiological pH were studied by spectroscopic methods. Fuorescence results revealed the formation of BSA–U(VI) complex, the binding constants as well as the number of binding sites were determined. In particular, the effects of U(VI) binding on the secondary structures of BSA were examined by means of Fourier transformation infrared spectroscopy equipped with attenuated total reflection (FT-IR/ATR). It was found that the α-helix component of BSA decreased gradually with increasing concentration of U(VI). In contrast, the β-sheets, turns, and random coil structures all increased correspondingly. Our work would shed light on the possible interaction mechanism between U(VI) and proteins in aqueous solutions.
通讯机构：
[Wang, Yanfei] U;Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
关键词：
Dry and wet immobilized Saccharomyces cerevisiae;Adsorption;Uranium
摘要：
Immobilized Saccharomyces cerevisiae (ISC) was prepared by the sodium alginate–gelatin embedding method after dry cells had been cross-linked by formaldehyde. Adsorption of uranium(VI) by incompletely and completely dry ISC was studied. The results indicated that incompletely dry ISC had greater adsorption capacity for U(VI), with physical adsorption being the primary mechanism, whereas completely dry ISC exhibited much greater rigidity and much smaller volume. Therefore, initial absorption of U(VI) by incompletely dry ISC followed by heating could be compared with glass solidification for disposal of radioactive waste. The influence of solution pH, temperature, and contact time on U(VI) absorption was also studied, with pH being found to be the main influencing factor. The adsorption mechanism of completely dry ISC was explored by scanning electron microscopy (SEM) and Fourier-transform infrared (FTIR) spectroscopy, indicating that the main adsorption mechanism is chemical adsorption.
作者：
Wang, X. -F.*;Chen, Z. -Q.;Yang, P. -F.;Wang, Y. -F.
期刊：
Russian Journal of Coordination Chemistry,2012年38(12):733-737 ISSN：1070-3284
通讯作者：
Wang, X. -F.
作者机构：
[Wang, Y. -F.; Wang, X. -F.; Chen, Z. -Q.; Yang, P. -F.] Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Wang, X. -F.] U;Univ S China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
关键词：
Pyridyl;Nitroxide;Oxyl;Cobalt Atom;Nitronyl
摘要：
A new cobalt coordination compound with nitronyl nitroxide and benzonate, Co(PyNIT)2(PhCOO)2(H2O)2, where PyNIT = 2-(p-pyridyl)-4,4′,5,5′-tetramethylimidazoline-1-oxyl-3-oxide, was synthesized and its X-ray diffraction analysis was carried out. The crystals were triclinic: a = 6.7749(4), b = 11.3472(7), c = 12.9066(8) Å, α = 87.906(1)°, β = 88.631(1)°, γ = 79.080(1)°, V = 973.45(10) Å3, space group P
$$
\bar 1
$$
, Z = 1, ρcalcd = 1.375 mg/m3, R = 0.0316. The environment of the cobalt atom is distorted octahedral formed by two oxygen atoms of two carboxylate groups of benzoate, two oxygen atoms of waters and two nitrogen atoms from two pyridyl nitronyl nitroxide molecules.
作者机构：
[Wang XiaoLiang; Liu YaLan; Wang YanFei] Univ S China, Sch Chem & Chem Engn, Dept Appl Chem, Hengyang 421001, Peoples R China.;[Li ZiJie; Lan JianHui; Wang XiaoLiang; Yuan LiYong; Zhao YuLiang; Liu YaLan; Feng YiXiao; Shi WeiQun; Chai ZhiFang] Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Analyt Techn, Beijing 100049, Peoples R China.;[Li ZiJie; Lan JianHui; Yuan LiYong; Zhao YuLiang; Feng YiXiao; Shi WeiQun] Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat Nanosafety, Beijing 100049, Peoples R China.
通讯机构：
[Wang YanFei] U;Univ S China, Sch Chem & Chem Engn, Dept Appl Chem, Hengyang 421001, Peoples R China.
作者：
He Ting-Ting;Li Bai;Xu Dian-Dou;Yang Xiao-Zhi;Ma Ling-Ling;...
期刊：
分析化学,2011年39(4):491-495 ISSN：0253-3820
通讯作者：
Wang Yan-Fei
作者机构：
[Yang Xiao-Zhi; He Ting-Ting; Wang Yan-Fei] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Li Bai; Xu Dian-Dou; Yang Xiao-Zhi; Wang Hua-Jian; He Ting-Ting; Ma Ling-Ling] Chinese Acad Sci, Key Lab Nucl Anal, Inst High Energy Phys, Beijing 100049, Peoples R China.
通讯机构：
[Wang Yan-Fei] U;Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Peoples R China.
