国家医学教育标准视野下本科临床教学质量相关因素分析
作者：
张新华;唐志晗;文格波;吴移谋;姜志胜
期刊：
中国高等医学教育 ,2011年(5):53-55 ISSN：1002-1701
作者机构：
南华大学医学院,湖南,衡阳,421001
关键词：
医学教育;临床教学;质量评价
摘要：
根据教学质量的一般性规定,结合国家临床医学专业标准要求,从满足学生、医院和患者三个层次的需求,分析提出临床教学质量相关因素是学生的学业成绩、学科性竞赛成绩、思想道德素质表现、考研率、就业率、就业质量、执业资格考试通过率、"三基"考试与规范化培训成绩、毕业后工作质量等。
语种：
中文
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靶向PRMT2基因的micro RNA对雌激素介导的乳腺癌MCF7细胞增殖的影响
作者：
钟警;李蓉芳;陈亚军;曹仁贤;文格波
期刊：
肿瘤 ,2011年31(11):965-971 ISSN：1000-7431
通讯作者：
Wen, G.-B.
作者机构：
南华大学第一附属医院临床医学研究所,衡阳,421001;[李蓉芳; 文格波; 曹仁贤; 钟警; 陈亚军] 南华大学第一附属医院
通讯机构：
Institute of Clinical Medicine, Nanhua University, China
关键词：
乳腺肿瘤;微小RNA类;蛋白精氨酸N-甲基转移酶;雌激素受体alpha;转染;细胞增殖
摘要：
目的:构建靶向人蛋白质精氨酸甲基转移酶 2 （protein arginine methyltransferase2,PRMT2）基因的microRNA真核表达载体,鉴定其稳定转染乳腺癌细胞株MCF7后对细胞增殖的影响。方法:根据PRMT2mRNA序列设计合成pre-microRNA片段,定向克隆至pcDNATM6.2-GW/EmGFP-miR真核表达载体,并稳定转染入MCF7细胞。采用间接免疫荧光法和蛋白质印迹法检测重组体对PRMT2表达的干扰效果以确定其生物活性。采用结晶紫实验和平板克隆形成实验检测重组体转染对MCF7细胞体外增殖和细胞克隆形成能力的影响。FCM法检测重组体转染对MCF7细胞周期的影响。结果:构建的重组体插入片段的碱基序列完全正确,并且重组体稳定转染MCF7细胞后可成功干扰PRMT2基因的表达。在无处理因子的情况下,重组体转染对细胞的生长速度无明显影响;与转染空载体的MCF7细胞相比,稳定转染重组体的MCF7细胞对雌激素的敏感性增强,但其对雌激素拮抗剂4-OHT处理的敏感性无明显差异。平板克隆形成实验表明,重组体能够增强雌激素介导的MCF7细胞的克隆形成能力。FCM法检测表明,转染重组体的MCF7细胞中G2期细胞比例明显升高（P<0.05）。结论:成功构建靶向PRMT2基因的pcDNATM6.2-GW/EmGFP-miR真核表达载体,且在稳定转染MCF7细胞后具有生物学活性。抑制内源性PRMT2基因的表达能够提高雌激素介导的MCF7细胞的增殖和克隆形成能力,上调雌激素受体α目标基因cyclinD1和c-myc的表达,促进细胞周期进程。
语种：
中文
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Effects of Aminoguanidine and vitamin C on collagen type IV in diabetic nephropathy rats
作者：
Li, Qiangxiang;Ao, Xiang;Du, Youhong;Li, Yang;Ou, Yangshi;...
期刊：
ENDOCRINE ,2011年39(3):251-258 ISSN：1355-008X
通讯作者：
Wen, Gebo
作者机构：
[Wen, Gebo; Li, Qiangxiang] NanHua Univ, Affiliated Hosp 1, Clin Res Inst, Henyang 421001, Hunan, Peoples R China.;[Ao, Xiang] Cent S Univ, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China.;[Du, Youhong; Li, Qiangxiang; Gong, Raofeng; Sun, Xuexiong; Yang, Yi Xiang] Loudi Municipal Cent Hosp Hunan, Loudi 417000, Peoples R China.;[Li, Yang] Maternal & Child Hlth Hosp Hunan Prov, Changsha 410008, Hunan, Peoples R China.;[Ou, Yangshi] Liberat Army 458 Hosp Guangdong Prov, Guangzhou 510602, Guangdong, Peoples R China.
通讯机构：
[Wen, Gebo] N;NanHua Univ, Affiliated Hosp 1, Clin Res Inst, Henyang 421001, Hunan, Peoples R China.
关键词：
Aminoguanidine;Vitamin C;Diabetic nephropathy;Type IV collagen
摘要：
The aim of this article is to investigate the effects of Aminoguanidine and vitamin C (VitC) on type IV collagen in diabetic nephropathy rats. Diabetic nephropathy rats were induced by intraperitoneal injection of STZ. Rats were randomly divided into five groups: normal control group (n = 10), diabetes group (n = 10), aminoguanidine group (n = 10), VitC group (n = 10), aminoguanidine and VitC group (n = 10). After 16 weeks, the general conditions, blood gloucose, glycosylated hemoglobin, blood urea nitrogen, serum creatinine, serum type IV collagen, urinary albumin excretion rate, and creatinine clearance rate were detected, type IV collagen protein was determined by immunohistochemical analysis as well as the expression of collagen type IVα1 mRNA were determined by in situ hybridization analysis in the kidneys of each group. The results were (1) diabetes mellitus and renal lesions occurred in the diabetes group, aminoguanidine group, VitC group, VitC and aminoguanidine group; (2) aminoguanidine and VitC improved the general conditions of diabetic nephropathy rats, decreased blood urea nitrogen, serum creatinine, and urinary albumin excretion rate as well as increased creatinine clearance rate. The expressions of collagen type IV were significantly down-regulated in treatment groups in contrast to the diabetes group. Aminoguanidine and VitC protect renal lesions in diabetic nephropathy, respectively, by inhibiting expression of type IV collagen, while aminoguanidine and VitC have a synergistic effect on them. © Springer Science+Business Media, LLC 2010.
语种：
英文
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Construction of eukaryotic expression vector of human S100A13 gene and its effect on proliferation of human thyroid cancer cell line TT
作者：
Xue-hui Xu;Ren-xian Cao;Ying-lan Liu;Jing Zhong;Ge-bo Wen
期刊：
中国现代医学杂志 ,2011年21(3):321-329 ISSN：1005-8982
作者机构：
[Xue-hui Xu; Ren-xian Cao; Ge-bo Wen] Clinical Medical Research Institute,the First Affiliated Hospital,University of South China;[Ying-lan Liu; Jing Zhong] Department of Pathophysiology,University of South China
关键词：
S100A13 gene;TT cells;gene transfection;cell proliferation;cell cycle
摘要：
Objective To investigate the effect of exogenous S100A13 gene overexpression on the proliferation of human thyroid cancer cell line TT.Methods The recombinant ORF of S100A13 tagged with six histidines at the 5' end was subcloned into the pcDNA3.2NV5/GW/D-TOPO vector and sequenced.The eukaryotic expression plasmid pcDNA3.2/V5 /GW/D-S100A13 and empty vector pcDNA3.2/V5/GW/D were transfected into TT cells.The positive clones were selected by G418.The expressions of S100A13 mRNA and protein were detected by real-time reverse transcription-polymerase chain reaction (RT-PCR)and Western blot.The effect of S100A13 on cell proliferation and cell cycle was evaluated by cell growth curve,MTT colorimetric assay and flow cytometry.Results S100A13 gene tagged with six histidines at the 5' end was confirmed to be inserted into the pcDNA3.2/V5/GW/D vector correctly.TT-S100A13-V5 cells,which over-expressed S100A13,were constructed successfully.TT-S100A13-V5 cells grew much faster than TT-V5 and TT cells (P <0.001).The proportions of both S and G2/M phase cells were significantly higher in TT-S100A13-V5 cells than those in TT-V5 and TT cells (P <0.001).Conclusion The eukaryotic expression vector containing human S100A13 gene has been successfully constructed,which highly expresses S100A13 in TT cells.Exogenous S100A13 gene overexpression accelerates TT cell proliferation and drives the cell cycle progression of TT cells from G0/G1 phase to S and G2/M phases.
语种：
英文
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Identification and expression analysis of a novel transcript of the human PRMT2 gene resulted from alternative polyadenylation in breast cancer
作者：
Zhong, Jing;Cao, Ren-Xian;Hong, Tao;Yang, Jing;Zu, Xu-Yu;...
期刊：
Gene ,2011年487(1):1-9 ISSN：0378-1119
通讯作者：
Wen, Ge-Bo
作者机构：
[Hong, Tao; Zhong, Jing; Wen, Ge-Bo; Xiao, Xin-Hua; Yang, Jing; Cao, Ren-Xian; Zu, Xu-Yu; Liu, Jiang-Hua] Univ S China, Affiliated Hosp 1, Clin Med Res Inst, Hengyang 421001, Peoples R China.;[Hong, Tao; Zhong, Jing; Wen, Ge-Bo; Yang, Jing; Cao, Ren-Xian] Univ S China, Dept Pathophysiol, Hengyang 421001, Peoples R China.
通讯机构：
[Wen, Ge-Bo] U;Univ S China, Affiliated Hosp 1, Clin Med Res Inst, Hengyang 421001, Peoples R China.
关键词：
Intron retention;Polyadenylation;PRMT2;Splicing;Subcellular localization;Up-regulated expression
摘要：
The arginine N-methyltransferase 2 protein (PRMT2, also known as HRMT1L1) is thought to act as a coactivator of ERα. The present results show the occurrence of a novel transcript by alternative polyadenylation in the human PRMT2 gene. We demonstrated that the newly identified intron-retaining PRMT2L2 transcript is functionally intact, efficiently translated into protein in vivo. PRMT2 and PRMT2L2 mRNA expression profiles overlap with the distribution of ERα, with the strongest abundance in estrogen target tissues. Transient co-transfection assays demonstrated that PRMT2L2 enhance ERα-mediated transactivation activity of ERE-Luc in a ligand-dependent manner. Confocal microscopy scanning revealed a distinct intra-cellular localization of their fusion proteins, suggesting that the C-terminal region absent in PRMT2L2 is critical for the localization. Statistical analysis further showed that both PRMT2 and PRMT2L2 mRNAexpressions were up-regulated in breast cancer tissues, and significantly associated with ERα positivity status. Thus, post-transcriptional processing mechanism as alternative polyadenylation and splicing may play a crucial role in regulating human PRMT2 gene expression. © 2011 Elsevier B.V.
语种：
英文
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基于国家医学教育标准的人文素质教育课程体系探讨
作者：
张新华;张天成;唐志晗;吴移谋;文格波;...
期刊：
中国高等医学教育 ,2010年(2):81-83 ISSN：1002-1701
作者机构：
南华大学医学院,湖南,衡阳,421001
关键词：
医学生;人文素质;课程体系
摘要：
分析了国家医学教育标准关于人文素质教育的内容与要求,从5个方面提出了医学生人文素质教育课程体系的构建思路,即明确设置范围、定位层次结构、确定组织形式、优化教学内容、注重序贯安排.
语种：
中文
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特色专业视野下临床医学专业建设的探索与实践
作者：
文格波;张新华;唐志晗;张天成;吴移谋;...
期刊：
中华医学教育杂志 ,2010年30(06):826-827,842 ISSN：1673-677X
作者机构：
南华大学医学院,衡阳,421001;南华大学医学院临床技能教学中心,衡阳,421001
关键词：
特色专业建设;临床医学;探索;实践
摘要：
在深化医疗卫生体制改革、促进医疗卫生事业发展中,临床医学专业人才发挥着重要的作用.根据特色专业建设的目标和原则,参照国家医学教育标准,结合社会需求和教学实际,南华大学医学院调整临床医学专业人才培养目标和专业方向,突出医学人文教育和临床技能培养,强化学科建设和优秀团队建设,创新教学模式和教学方法,以期提高专业人才的培养质量.
语种：
中文
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Effect of Human S100A13 Gene Silencing on FGF-1 Transportation in Human Endothelial Cells
作者：
Cao, Renxian;Yan, Bin;Yang, Huiling;Zu, Xuyu;Wen, Gebo* ;...
期刊：
Journal of the Formosan Medical Association ,2010年109(9):632-640 ISSN：0929-6646
通讯作者：
Wen, Gebo
作者机构：
[Wen, Gebo; Cao, Renxian] Univ S China, Dept Pathophysiol, Hengyang, Peoples R China.;[Zhong, Jing; Yang, Huiling; Yan, Bin; Zu, Xuyu] Univ S China, Clin Med Res Inst, Affiliated Hosp 1, Hengyang, Peoples R China.
通讯机构：
[Wen, Gebo] U;Univ S China, Dept Pathophysiol, Hengyang, Peoples R China.
关键词：
FGF-1;lentiviral vector;RNAi;S100A13;shRNA
摘要：
Background/Purpose: The S100 protein is part of a Ca 2+ binding protein superfamily that contains an EF hand domain, which is involved in the onset and progression of many human diseases, especially the proliferation and metastasis of tumors. S100A13, a new member of the S100 protein family, is a requisite component of the fibroblast growth factor-1 (FGF-1) protein release complex, and is involved in human tumorigenesis by interacting with FGF-1 and interleukin-1. In this study, experiments were designed to determine the direct role of S100A13 in FGF-1 protein release and transportation. Methods: We successfully constructed the lentiviral vectors containing shRNA targeting the human S100A13 gene. Human umbilical vein endothelial cells (HUVECs) were transfected with lentiviral RNAi vectors for S100A13. Then immunofluorescence staining, real-time quantitative polymerase chain reaction and Western blotting were used to detect the inhibition efficiency of the vectors and to monitor the release and transportation of FGF-1 protein. Results: Lentiviral RNAi vectors induced suppression efficiency of S100A13 gene by 90% in HUVECs. FGF-1 protein was found to be transported from the cytoplasm to the cell membrane, and then released from cells when HUVECs were deprived of serum. The release of FGF-1 protein was blocked by the downregulation of S100A13, but the transportation was not affected, suggesting that S100A13 is a key cargo protein for FGF-1 release. Conclusion: S100A13 promotes the release of FGF-1 protein, but does not affect the transportation of FGF-1 protein in HUVECs. © 2010 Formosan Medical Association & Elsevier.
语种：
英文
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围绕本科医学教育标准 创新临床技能培养模式
作者：
唐志晗;文格波;文红艳;张新华;吴移谋
期刊：
中国高等医学教育 ,2010年(3):8-9+21 ISSN：1002-1701
作者机构：
南华大学医学院,湖南,衡阳,421001;[唐志晗; 文格波; 吴移谋; 文红艳; 张新华] 南华大学
关键词：
医学教育标准;临床技能;创新;培养模式
摘要：
《本科医学教育标准——临床医学专业（试行）》的颁布，为进一步提高医学教学质量，规范医学教育管理具有重要的现实意义。医学生的临床技能培养一直是高等医学教育的薄弱环节，如何紧紧围绕最新《标准》，创新临床技能的培养模式是一项急需研究的课题。通过分析我国临床技能培养目前的遭遇困境，并联系我校临床技能培养的实际，结合《标准》要求，对临床技能培养模式的创新进行了探讨。
语种：
中文
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医学专业课程中渗透人文教育探析
作者：
张新华;唐志晗;吴移谋;文格波;张天成;...
期刊：
中华医学教育杂志 ,2010年30(5):701-703 ISSN：1673-677X
作者机构：
南华大学医学院临床技能教学中心,衡阳,421001;南华大学医学院;[唐志晗; 文格波; 张天成; 吴移谋; 姜志胜; 张新华] 南华大学
关键词：
人文素质;医学专业课程;渗透教育
摘要：
医学专业课程中渗透人文教育是实施医学人文素质教育的"长线"途径和有效方式。本文提出了渗透教学的目的性、侧重性、适度性、关联性原则,以及链接名人故事、联系现实问题、运用特定仪式、利用教学形式等方法,指出了医学专业课程中人文教育的要点和教师主导作用发挥的途径。
语种：
中文
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肿瘤相关基因的选择性剪接
作者：
刘薇;钟警;文格波
期刊：
社区医学杂志 ,2010年8(1):34-35 ISSN：1672-4208
作者机构：
南华大学临床医学研究所,湖南衡阳,421001;南华大学附属第一医院内分泌科,湖南衡阳,421001
关键词：
选择性剪接;肿瘤相关基因;组织特异性表达;剪接变异体;可变剪接;剪接位点;疾病发生
摘要：
选择性剪接(alternative splicing)又称变位剪接或可变剪接,指的是从一个mRNA前体通过选择不同的剪接位点组合产生不同的mRNA剪接变异体的过程.该过程是生物界普遍存在的现象,它在机体发育、组织特异性表达、疾病发生和发展等方面起重要作用,是生物体内一种基本且重要的调控机制.近年来,越来越多的研究显示,在人类肿瘤发生发展过程中RNA的选择性剪接扮演着重要的角色.
语种：
中文
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医学生临床技能教学与考核新模式的构想与实践
作者：
尹凯;桂庆军;文格波;涂玉林;姜志胜
期刊：
中国高等医学教育 ,2009年(12):21-22 ISSN：1002-1701
作者机构：
南华大学医学院,湖南,衡阳,421001;[文格波; 涂玉林; 桂庆军; 姜志胜; 尹凯] 南华大学
关键词：
临床技能;教学;考核;新模式;实践
摘要：
临床技能实践教学是现代医学教育的重要内容,是实现《中国本科医学教育标准》的关键环节。然而,传统医学生临床技能培养模式存在教学内容重复、教学资源分散和考核手段单一等问题。为克服传统教学模式的弊端,我校对临床技能教学与考核模式进行了创新性改革与实践,取得了良好教学效果。
语种：
中文
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过表达S100A13基因对甲状腺癌TT细胞增殖特性的影响
作者：
曹仁贤;田利娜;文芳;刘星;钟警;...
期刊：
CANCER COMMUNICATIONS ,2008年27(8):822-827 ISSN：1000-467X
通讯作者：
Cao, R.X.
作者机构：
[曹仁贤; 田利娜; 文芳; 刘星; 钟警; 文格波] 南华大学附属第一医院临床学研究所
关键词：
S100A13基因;甲状腺肿瘤;TT细胞;基因转染;细胞增殖;细胞周期
摘要：
背景与目的：有研究表明，S100A13基因与肿瘤的发生有关，而S100A13基因在人甲状腺组织中高表达。本研究旨在探讨S100A13基因过表达对甲状腺癌TT细胞增殖特性的影响。方法：应用Lipofectamine 2000将真核表达载体pCDNA3．1／NT—GFP—S100A13和空载体pCDNA3．1／NT-GFP导入TT细胞，经G418抗性筛选得到稳定的克隆并扩大培养成细胞系，激光共聚焦显微镜观察外源性S100A13蛋白在细胞中的定位。采用real—time RT-PCR和Western blot鉴定稳定过表达S100A13的TT细胞。分别应用细胞生长曲线、流式细胞术方法检测过表达S100A13基因对TT细胞生长速率和细胞周期的影响。结果：成功建立了稳定过表达S100A13和空载体的细胞系IT-S100A13-GFP、IT—GFP。分别将1×10~4个IT-S100A13-GFP、TT—GFP和TT细胞培养7d后，各组细胞数目分别为（2．30±0．24）×10~5个、（1．40±0．25）×10~5个和（1．50±2．20）×10~5个（P〈0．05）；TT-S100A13-GFP、TT-GFP和TT细胞经流式细胞仪检测S期的细胞比例分别为（6．47±0．14）％、（5．86±0．23）％和（5．99±0．28）％（P〈0．05），G_2／M期的细胞比例分别为（50．27±0．66）％、（39．39±0．23）％和（39．64±0．64）％（P〈0．05）。结论：过表达S100A13基因对甲状腺癌TT细胞的增殖具有促进作用。促进TT细胞周期从G_0／G_1期向S期及G_2／M期过渡。
语种：
中文
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Small Interfering RNA-mediated Caveolin-1 Knockout on Plasminogen Activator Inhibitor-1 Expression in Insulin-stimulated Human Vascular Endothelial Cells
作者：
Yang, Huiling;He, Shuya;Quan, Zhihua* ;Peng, Weixia;Yan, Bin;...
期刊：
生物化学与生物物理学报 ,2007年39(3):224-233 ISSN：1672-9145
通讯作者：
Quan, Zhihua
作者机构：
[Quan, Zhihua] Nanhua Univ, Affiliated Hosp 1, Inst Clin Med, Hengyang 421001, Peoples R China.;Cent S Univ, Res Inst Biochem & Mol Biol, Changsha 421001, Peoples R China.;Nanhua Univ, Inst Pharmacol & Pharm, Hengyang 421001, Peoples R China.
通讯机构：
[Quan, Zhihua] N;Nanhua Univ, Affiliated Hosp 1, Inst Clin Med, Hengyang 421001, Peoples R China.
关键词：
caveolin-1;plasminogen activator inhibitor-1;gene silencing;insulin
摘要：
Using human vascular endothelial cells (ECV304) as the target, we studied the effect of caveolin (CAV)-1 in the course of insulin-stimulated expression of plasminogen activator inhibitor (PAI)-1. The appropriate single-stranded oligonucleotides representing the RNAi CAV-1 gene were analyzed by Ambion software. After annealing to generate double-stranded oligonucleotides (ds oligo), it was cloned into the pENTR/U6 entry vector containing RNA polymerase III expression element by T4 DNA ligase. The short hairpin (shRNA) sequences transferred from the pENTR/U6 entry were cloned into the pLenti6/BLOCK-iT-DEST vector with an LR recombination reaction. After identification by sequencing, we successfully constructed the CAV-1 RNAi lentiviral expression system using Gateway technology. Silencing efficiency was assayed by real-time reverse transcription-polymerase chain reaction, immunofluorescence staining and Western blotting. ECV304 cells were cultured in the medium containing different concentrations of insulin (1×10-9 to 1×10-7 M) with the CAV-1 gene silenced or not. The expression level and subcellular localization of PAI-1 and CAV-1 were compared using reverse transcription- polymerase chain reaction, immunofluorescence staining and Western blot assay. The results showed that the potent inhibition of CAV-1 expression could reach 85%, and it was specific to the CAV-1-derived shRNA, not the S100A13-derived shRNA. There was no dramatic difference in PAI-1 expression between the RNAi+ and RNAi- ECV304 cells incubated with physiological insulin, but PAI-1 protein did accumulate under the cell membrane. As the concentration of insulin increased, the expression of PAI-1 was up-regulated, whereas the expression of CAV-1 attenuated. Furthermore, PAI-1 clearly augmented after CAV-1 knockdown. These results indicated that hyperinsulinism could promote PAI-1 expression by inhibiting CAV-1, and stabilizing or up-regulating CAV-1 expression in endothelial cells might reduce complications of the great vessels and capillary vessels in diabetes. © Institute of Biochemistry and Cell Biology, SIBS, CAS.
语种：
英文
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Construction and Functional Analysis of a Lentiviral Expression Vector Containing- a Scavenger Receptor (SR-PSOX) that Binds Uniquely Phosphatidylserine and Oxidized Lipoprotein
作者：
Quan, Zhihua;Yang, Huiling;Yang, Yongzong* ;Yan, Bin;Cao, Renxian;...
期刊：
生物化学与生物物理学报 ,2007年39(3):208-216 ISSN：1672-9145
通讯作者：
Yang, Yongzong
作者机构：
[Yang, Yongzong] Nanhua Univ, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.;Nanhua Univ, Affiliated Hosp 1, Inst Clin Med, Hengyang 421001, Peoples R China.
通讯机构：
[Yang, Yongzong] N;Nanhua Univ, Inst Cardiovasc Dis, Hengyang 421001, Peoples R China.
关键词：
SR-PSOX;lentiviral expression vector;atherosclerosis;TNF-α
摘要：
The aim of this study is to construct a lentiviral expression vector containing a scavenger receptor (SR-PSOX) that binds with uniquely phosphatidylserine and oxidized lipoprotein with six histidine tags and to investigate the function of SR-PSOX in atherosclerosis. We utilize the ViraPower lentiviral expression system which was efficient to deliver in vitro or in vivo the target gene into dividing and nondividing mammalian cells using an enhanced biosafety replication-incompetent lentivirus. The blunt-end sequence was amplified using the reverse transcription-polymerase chain reaction and directional TOPO cloning reaction. Through a pair of the cytomegalovirus forward primer and the reverse primer of SR-PSOX, the correct clones were identified by polymerase chain reaction and sequencing. The ViraPower packaging mix and SR-PSOX-pLenti6/V5 TOPO expression plasmid were co-transfected into the 293FT cell line using Lipofectamine 2000. The expression of endogenous and exogenous SR-PSOX as well as tumor necrosis factor (TNF)-α protein in various foam cell models at different time points were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blot and indirect immunofluorescence assay. Western blot and immunofluorescence analysis confirmed that the expressions of SR-PSOX and TNF-α protein were upregulated in foam cell models. Our data suggested that the overexpression of recombinant human SR-PSOX protein can promote foam cell formation and upregulate the expression of the inflammatory factor TNF-α. © Institute of Biochemistry and Cell Biology, SIBS, CAS.
语种：
英文
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脉动热管启动过程的实验研究
作者：
徐荣吉;王瑞祥;丛伟;任挪颖;吴业正
期刊：
西安交通大学学报 ,2007年41(5):530-533 ISSN：0253-987X
通讯作者：
Xu, R.
作者机构：
[吴业正; 王瑞祥; 丛伟; 徐荣吉; 任挪颖] Department of Refrigerating and Cryogenic Engineering, Xi'an Jiaotong University, Xi'an 710049, China;[王瑞祥] Department of Metropolitan Construction Engineering, Beijing University of Civil Engineering and Architecture, Beijing 100044, China
通讯机构：
Department of Refrigerating and Cryogenic Engineering, Xi'an Jiaotong University, China
关键词：
脉动热管;启动过程;实验研究
摘要：
为了使电子设备在启动过程中具有更好的稳定性,采用控制恒定热流密度和冷凝温度的方法对平板开槽型脉动热管的启动过程进行了系统的实验研究．实验发现:在特定工况下,启动过程中脉动热管管内工质会突然剧烈沸腾,此时脉动热管蒸发段温度突然降低,冷凝段温度突然升高,广义热阻突然降低．针对本实验装置,存在一个使脉动热管启动迅速而且达到稳定运行状态时广义热阻最小的最佳充灌率(50%)、最佳管体倾斜角度(50°)和最佳加热功率(28．5 W)．热管冷却段冷却水流量对脉动热管启动性能影响较小．
语种：
中文
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氨基胍和维生素C对糖尿病大鼠血脂水平和主动脉硫酸乙酰肝素蛋白多糖表达的影响
作者：
李强翔;文格波;王琳娜;肖扬;陈梅贵;...
期刊：
中国药理学与毒理学杂志 ,2006年20(5):387-392 ISSN：1000-3002
通讯作者：
Li, Q.-X.
作者机构：
[陈梅贵; 张卓; 肖扬] Loudi Municipal Central Hospital of Hunan Province, Loudi 417000, China;[文格波] Clinical Research Institute, First Affiliated Hospital, Nanhua University, Henyang 421001, China;[钟惠菊; 王琳娜] Xiangya Hospital, Central South University, Changsha 410008, China;[李强翔] Loudi Municipal Central Hospital of Hunan Province, Loudi 417000, China, Xiangya Hospital, Central South University, Changsha 410008, China
通讯机构：
[Li, Q.-X.] L;Loudi Municipal Central Hospital of Hunan Province, Loudi 417000, China
关键词：
氨基胍;维生素C;糖尿病;实验性;脂蛋白类;主动脉;硫酸乙酰肝素蛋白多糖
摘要：
目的 观察氨基胍(Ami)与维生素C(VitC)合用是否可通过抑制糖基化和氧化应激对糖尿病大鼠主动脉起到保护作用。方法 腹腔注射链脲佐菌素诱导建立1型糖尿病大鼠模型，Ami,VitC和VitC+Ami治疗组分别ig Ami 100mg·kg~(-1)，VitC100 mg·kg~(-1)或VitC 100 mg·kg~(-1)+ Ami 100 mg·kg~(-1)，每日1次，给药16周。观察大鼠的一般状况；血糖、血清甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、糖化血红蛋白（HbA1c）和糖化低密度脂蛋白（GLDL）水平；HE染色及免疫组织化学检测主动脉内膜硫酸乙酰肝素蛋白多糖（HSPG）表达。结果 与模型组相比，Ami和VitC可增加糖尿病大鼠的体重，但对血糖水平无影响；VitC 降低TG 、TC和LDL水平，显著提高HDL水平，Ami 及VitC 明显降低HbA1c和GLDL水平，并增强主动脉HSPG表达。Ami+VitC 的治疗作用较Ami及VitC更为明显，但所有的观察指标未恢复至正常组水平。结论 Ami和VitC无降血糖作用，但可通过抑制糖基化和氧化应激对糖尿病大鼠主动脉起到保护作用。
语种：
中文
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Effect of vitamin C on the expression of collagen IV alpha 1 mRNA in rats with diabetic nephropathy
作者：
李强翔;雷小勇;谢小英;欧玉兰;陈梅贵;...
期刊：
中国组织工程研究 ,2005年9(39):75-77 ISSN：2095-4344
通讯作者：
Li, Q.-X.
作者机构：
[李强翔; 谢小英; 邓小金; 陈梅贵] Department of Endocrinology, Loudi Municipal Central Hospital, Loudi 417000 Hunan Province, China;[雷小勇] Department of Pharmacology, Nanhua University, Hengyang 421001 Hunan Province, China;[文格波; 欧玉兰] Institute of Clinical Medicine, Nanhua University, Hengyang 421001 Hunan Province, China
通讯机构：
[Li, Q.-X.] D;Department of Endocrinology, Loudi Municipal Central Hospital, China
关键词：
维生素C;糖尿病肾病模型;Ⅳ型胶原α1mRNA;基因表达
摘要：
目的:观察维生素C对糖尿病肾病大鼠Ⅳ型胶原α1mRNA表达及肾脏功能的影响.方法:实验于2004-01/04在南华大学动物部完成.大鼠预养1周后,空腹12 h,腹腔注射60mg/kg链尿佐菌素-柠檬酸钠缓冲液,对照组注射等量柠檬酸钠缓冲液,依据72 h后尿糖()～(),静脉采血测血糖≥16.7 mmol/L,确定建立Ⅰ型糖尿病大鼠模型.将SD大鼠随机分为正常对照组、糖尿病组和维生素C组,保持大鼠血糖波动在25 mmol/L左右,持续16周.观察治疗期间及治疗后大鼠的一般状况、血糖、尿素氮、血肌酐,内生肌酐清除率、24 h尿蛋白排泄率,原位杂交检测肾组织Ⅳ型胶原α1基因表达.结果:30只SD大鼠实验过程中无死亡,全部纳入结果分析.①大鼠体质量较正常对照组明显下降,尿量增加,血糖升高,差异有显著性意义(t=4.945,3.779,P＜0.05),治疗后维生素C组与糖尿病组相比,体质量、肾质量/体质量有显著提高(t=2.927,3.032,P＜0.05).②大鼠血清尿白蛋白排泄率、血肌酐、尿素氮较正常对照组明显升高,内生肌酐清除率下降,有显著性差异(t=4.113,3.1251,2.798,P＜0.05),糖尿病组与维生素C组相比,尿白蛋白排泄率、血肌酐、尿素氮明显升高,内生肌酐清除率下降(t=2.875,2.994,3.102,P＜0.05).③与正常对照组相比,肾小球血管细胞外基质Ⅳ型胶原α1基因表达显著上调,经灰度值测定两组间差异有显著性意义(P＜0.05,t=4.766),治疗后维生素C组肾组织着色浅淡稀疏,与糖尿病组相比Ⅳ型胶原α1基因相对表达明显下调(t=2.879,P＜0.05).结论:维生素C无降糖作用,但具有确切的肾脏保护作用.
语种：
中文
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糖尿病新西兰兔心、肾NOS和抗氧化酶的变化
作者：
唐朝克;梁丽红;徐刚;尹卫东;杨保堂;...
期刊：
中国病理生理杂志 ,2002年18(6):714-715,721 ISSN：1000-4718
作者机构：
南华大学基础医学院诊断学教研室;南华大学心血管病研究所,湖南,衡阳,421001;中南大学附属湘雅医院心血管内科,湖南,长沙,410008
关键词：
新西兰兔;糖尿病;一氧化氮合酶;抗氧化药
摘要：
目的:探讨糖尿病新西兰兔心、肾一氧化氮合酶(NOS)活力和抗氧化酶活力变化以及它们在糖尿病发病机制中的作用.方法:采用高脂高糖饲料喂养新西兰兔,建立一种新的糖尿病动物模型.观察糖尿病新西兰兔心、肾一氧化氮水平,一氧化氮合酶(NOS)活力及抗氧化酶活力的变化.结果:糖尿病新西兰兔出现大量蛋白尿(P＜0.05);肌酐清除率(CCr)明显高于对照组(P＜0.01);心脏组织中NO-2水平和NOS活力明显低于对照组(P＜0.05),而肾脏组织中NO-2水平和NOS活力明显高于对照组(P＜0.01);血糖及胰岛素明显高于对照组(P＜0.01);超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(catalase)活性都明显低于对照组(P＜0.01).结论:糖尿病新西兰兔心、肾中的NO-2水平和NOS活力异常,抗氧化酶活力明显降低,糖尿病的心、肾并发症可能与它们的异常变化有关.
语种：
中文
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糖尿病新西兰兔模型的建立及并发糖尿病肾病的机理研究
作者：
唐朝克;尹卫东;徐刚;梁丽红;杨保堂;...
期刊：
中南医学科学杂志 ,2001年29(5):439-442 ISSN：2095-1116
作者机构：
南华大学生理学教研室;[尹卫东; 文格波; 杨保堂; 徐刚; 唐朝克; 梁丽红] 南华大学
关键词：
新西兰兔;糖尿病;一氧化氮;平均动脉压;血糖
摘要：
目的用新西兰兔建立一种新的糖尿病动物模型,探讨一氧化氮在糖尿病肾病发病机制中的作用.方法采用高脂高糖饲料喂养新西兰兔,建立一种新的糖尿病动物模型. 观察糖尿病新西兰兔平均动脉血压、血脂、胰岛素、肾脏病变和血浆、肾组织中的一氧化氮水平及一氧化氮合酶(NOS)活力变化.结果糖尿病新西兰兔平均动脉血压增高(P <0.05); 且出现大量蛋白尿(P<0.05);肌酐清除率(CCr)明显增高(P<0.001);血浆及肾组织中NO水平和 NOS活力明显增高(P<0.001);血糖及胰岛素明显增高(P< 0.001).结论新西兰兔可用来建立糖尿病模型;N0是影响糖尿病新西兰兔肾小球高滤过的重要介质,肾脏NO系统异常与DN有关.
语种：
中文
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