先天性耳聋-甲状腺肿综合征一例报告及文献复习
作者：
向前;文格波;曹仁贤
期刊：
中国全科医学 ,2013年16(17):2048-2050 ISSN：1000-8179
作者机构：
[向前] 广东深圳市南山区人民医院;[文格波; 曹仁贤] 衡阳医学院
关键词：
先天性耳聋-甲状腺肿综合征;潘特林;听觉丧失,感音神经性;甲状腺肿
摘要：
先天性耳聋-甲状腺肿综合征又称Pendred综合征（PDS）,是一种罕见疾病,是以甲状腺肿大、感音神经性耳聋为主要特征的常染色体隐性遗传病.致病基因位于染色体7q,基因变异导致pendrin（潘特林）蛋白功能障碍,进而引起相应症状.本文结合相关文献指出了PDS的病因、临床表现及诊断要点,说明了当前研究进展及治疗方法,使临床医师对该病的认识进一步加强.
语种：
中文
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The physiological and pathophysiological roles of adipocyte miRNAs
作者：
Ling, Hongyan;Li, Xing;Yao, Chao Hua;Hu, Bi;Liao, Duanfang;...
期刊：
BIOCHEMISTRY AND CELL BIOLOGY ,2013年91(4):195-202 ISSN：0829-8211
通讯作者：
Zhang, Liang
作者机构：
[Li, Xing; Ling, Hongyan; Hu, Bi] Univ South China, Dept Physiol, Sch Med, Hengyang, Peoples R China.;[Ling, Hongyan] Univ South China, Ctr Postdoctoral Studies Basic Med, Hengyang, Peoples R China.;[Zhang, Liang; Yao, Chao Hua] Palmer Coll Chiropract Florida, PCCR, Palmer Lab Cell & Mol Biol, Port Orange, FL 32129 USA.;[Liao, Duanfang] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, State Key Lab Chinese Med Powder & Med Innovat Hu, Changsha, Hunan, Peoples R China.;[Feng, Shuidong] Univ South China, Dept Epidemiol, Hengyang, Peoples R China.
通讯机构：
[Zhang, Liang] P;Palmer Coll Chiropract Florida, PCCR, Palmer Lab Cell & Mol Biol, 4705 S Clyde Morris Blvd, Port Orange, FL 32129 USA.
关键词：
miRNAs;adipose tissue;adipogenesis;obesity;diabetes;insulin resistance;miARN;tissu adipeux;adipogenèse;obésité;diabète;résistance à l’insuline
摘要：
MicroRNAs (miRNAs) are highly conserved, small, noncoding RNAs that regulate gene expression at the posttranscriptional level. Their actions affect numerous important biological processes, including adipocyte differentiation and function, sugar and lipid metabolism, and insulin production and secretion. Recent reports suggest miRNAs may also be involved in the pathogenic processes of obesity, diabetes, and insulin resistance. In this review, we summarize research progresses on adipocyte miRNAs and their physiological and pathological implications. © 2013 Published by NRC Research Press.
语种：
英文
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现代教育技术在医学生临床基本技能教学中的应用探讨
作者：
梁丽红;桂庆军;文格波;沈元琼;尹凯;...
期刊：
医学教育研究与实践 ,2013年21(3):561-563 ISSN：2096-3181
作者机构：
南华大学医学院,湖南衡阳,421001
关键词：
现代教育技术;临床基本技能;立体化教学
摘要：
通过探讨基础仿真模拟技术、高级综合模拟人技术、多媒体教学技术、计算机辅助教学、网络教学等现代教育技术的特点,分析各种现代教育技术手段教学方法的优缺点,提出通过优化组合各技术手段方法,可以实现优势互补,相辅相成,从而建立临床基本技能的立体化教学模式,提高教学效果。
语种：
中文
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The relationship between bone marrow characteristics and the clinical prognosis of antithyroid drug-induced agranulocytosis
作者：
Yang, Jing;Zhong, Jing;Xiao, Xin-Hua;Zhou, Ling-Zhi;Chen, Ya-Jun;...
期刊：
Endocrine Journal ,2013年60(2):185-189 ISSN：0918-8959
通讯作者：
Wen, Ge-Bo
作者机构：
[Wen, Ge-Bo; Xiao, Xin-Hua; Yang, Jing; Cao, Ren-Xian; Liu, Jiang-Hua] Univ South China, Affiliated Hosp 1, Dept Endocrinol & Metab, Hengyang 421001, Peoples R China.;[Zhong, Jing; Wen, Ge-Bo; Zhou, Ling-Zhi] Univ South China, Affiliated Hosp 1, Clin Med Res Inst, Hengyang 421001, Peoples R China.;[Yang, Jing; Chen, Ya-Jun] Univ South China, Dept Pathophysiol, Hengyang 421001, Peoples R China.
通讯机构：
[Wen, Ge-Bo] U;Univ South China, Affiliated Hosp 1, Clin Med Res Inst, Hengyang 421001, Peoples R China.
关键词：
Antithyroid drug;Agranulocytosis;Bone marrow characteristic;Hyperthyroidism
摘要：
This study is aimed to explore the relationship between bone marrow characteristics and clinical prognosis of antithyroid drug (ATD) induced agranulocytosis. A retrospective study was conducted in the first affiliated hospital of the University of South China. A total of 33 hospitalized patients diagnosed with ATD-induced agranulocytosis were analyzed. The bone marrow characteristics were classified into two types. Type I was characterized by reduction or absence of granulocytic precursors and type II was recognized as hypercellular bone marrow with dysmaturity of granulocytic cells. Bone marrow of 20 cases (61%) were characterized with type I whereas 13 cases (39%) with type II. The median duration of neutrophil recovery and high-grade fever were 4.7 ± 1.0 days and 3.6 ± 2.5 days respectively for type II, compared to 8.0 ± 2.8 days and 8.6 ± 3.1 days for type I (p < 0.01 in both compared groups). However, there was no significant difference between the two types in terms of age, median duration of drug administration before the diagnosis of agranulocytosis, the amount of neutrophil count on admission and the total administration dose of granulocyte-colony stimulating factor (G-CSF) before bone marrow examination. Two cases of type I died of complications from infection. This study showed that the bone marrow characteristics of ATD-induced agranulocytosis could be classifed into two types. Also, the clinical prognosis was closely related to the bone marrow features. Type I is the dominant type which is usually associated with worse clinical prognosis compared to type II. ©The Japan Endocrine Society.
语种：
英文
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新形势下临床实践教学基地建设与管理的应对举措
作者：
张新华;文格波;张天成;唐志晗;杨科;...
期刊：
医学教育研究与实践 ,2012年20(4):680-681,684 ISSN：2096-3181
作者机构：
南华大学医学院,湖南衡阳,421001;[唐志晗; 文格波; 杨科; 吴移谋; 张天成; 张新华] 南华大学
关键词：
医学教育;临床教学基地;对策
摘要：
临床实践教学基地建设与管理是高等医学院校教学工作的重要组成部分.总体应坚持适应规模发展、合作互促双赢、注重规划设计、持续跟进加强的基本原则,具体在建设上应依据政策标准遴选临床教学基地,明确基本要求建设临床教学基地;在管理上应重点完善和优化组织管理体系、建立健全的临床教学管理机制、加强临床教学师资培训、规范和强化教学管理、改进和加强临床技能考核.经过持续加强建设和优化管理,提高了医学生的临床教学质量.
语种：
中文
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表观遗传调控在糖尿病及其并发症中的作用
作者：
Ling Hong-Yan;Hu Bi;Feng Shui-Dong;Liao Duan-Fang* ;Wen Ge-Bo
期刊：
生物化学与生物物理进展 ,2012年39(1):14-21 ISSN：1000-3282
通讯作者：
Liao Duan-Fang
作者机构：
[Ling Hong-Yan; Hu Bi] Univ S China, Dept Physiol, Sch Med, Hengyang 421001, Peoples R China.;[Liao Duan-Fang] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Ling Hong-Yan] Univ S China, Ctr Basic Med Postdoctoral Studies, Hengyang 421001, Peoples R China.;[Feng Shui-Dong] Univ S China, Dept Epidemiol, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Wen Ge-Bo] Univ S China, Inst Clin Res, Affiliated Hosp 1, Hengyang 421001, Peoples R China.
通讯机构：
[Liao Duan-Fang] H;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
关键词：
表观遗传调控;糖尿病;糖尿病并发症
摘要：
表观遗传(epigenetics)是指DNA序列不发生变化但基因表达却发生了可遗传的改变.表观遗传调控过程十分复杂,主要包括DNA甲基化、组蛋白修饰和微小RNA(miRNA)等.糖尿病是一种慢性代谢性疾病,常伴随大血管和微血管并发症.糖尿病的发生、发展不仅取决于遗传因素,而且也受到表观遗传修饰的调控.因此,对表观遗传调控的研究将为糖尿病及其并发症的预防和治疗提供新的思路和方法.
语种：
中文
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Identification and characterization of novel spliced variants of PRMT2 in breast carcinoma
作者：
Zhong, Jing;Cao, Ren-Xian;Zu, Xu-Yu;Hong, Tao;Yang, Jing;...
期刊：
FEBS JOURNAL ,2012年279(2):316-335 ISSN：1742-464X
通讯作者：
Wen, Ge-Bo
作者机构：
[Hong, Tao; Zhong, Jing; Wen, Ge-Bo; Liu, Ling; Xiao, Xin-Hua; Yang, Jing; Cao, Ren-Xian; Ding, Wen-Jun; Zu, Xu-Yu; Liu, Jiang-Hua] Univ S China, Affiliated Hosp 1, Clin Med Res Inst, Hengyang 421001, Peoples R China.;[Hong, Tao; Zhong, Jing; Wen, Ge-Bo; Yang, Jing; Cao, Ren-Xian] Univ S China, Dept Pathophysiol, Hengyang 421001, Peoples R China.;[Zhao, Qiang] Univ S China, Affiliated Hosp 1, Dept Pathol, Hengyang 421001, Peoples R China.
通讯机构：
[Wen, Ge-Bo] U;Univ S China, Affiliated Hosp 1, Clin Med Res Inst, Hengyang 421001, Peoples R China.
关键词：
breast cancer;ERα;PRMT2;splice variants;upregulated
摘要：
Protein N-arginine methyltransferases (PRMTs) participate in a number of cellular processes, including cell growth, nuclear/cytoplasmic protein shuttling, differentiation, RNA splicing and post-transcriptional regulation. PRMT2 (also known as HRMT1L1) is clearly involved in lung function, the inflammatory response, apoptosis promotion, Wnt signaling and leptin signaling regulation through different mechanisms. In this study, we report the molecular and cell biological characterization of three novel PRMT2 splice variants isolated from breast cancer cells and referred to as PRMT2α, PRMT2β and PRMT2γ. Compared with the wild-type PRMT2, these variants lack different motifs and therefore generate distinct C-terminal domains. Confocal microscopy scanning revealed a distinct intracellular localization of PRMT2 variants, suggesting that the alternatively spliced C-terminus of PRMT2 can directly influence its subcellular localization. Our findings reveal that these variants are capable of binding to estrogen receptor alpha (ERα) both in vitro and in vivo, and the N-terminal regions of these variants contribute to ERα-PRMT2 interactions. Furthermore, these variants were proved to be able to enhance ERα-mediated transactivation activity. Luciferase reporter assays showed that PRMT2s could modulate promoter activities of the ERα-targeted genes of Snail and E-cadherin. In addition, PRMT2 silencing could enhance 17β-estradiol-induced proliferation by regulating E2F1 expression and E2F1-responsive genes in ERα-positive breast cancer cells. Real-time PCR and immunohistochemistry showed that overall PRMT2 expression was upregulated in breast cancer tissues and significantly associated with ERα positivity status both in breast cancer cell lines and breast cancer tissues. We speculate that PRMT2 and its splice variants may directly modulate ERα signaling and play a role in the progression of breast cancer. Structured digital abstract and by () with by () with by () to by () with by () and by () Here we identified the molecular and cell biological characterization of three novel PRMT2 splice variants isolated from breast cancer cells. Coimmunoprecipitation assays in MCF7 cells and HepG2 cells, demonstrated that endogenous PRMT2 variants physically interacts with ERα/AR, respectively. Confocal microscopy suggested a colocalization of PRMT2 variants and ERα/AR in a nuclear area. © 2011 FEBS.
语种：
英文
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Transforming growth factor-β signaling in tumor initiation, progression and therapy in breast cancer: An update
作者：
Zu, Xuyu;Zhang, Qinghai;Cao, Renxian;Liu, Jianghua;Zhong, Jing;...
期刊：
Cell and Tissue Research ,2012年347(1):73-84 ISSN：0302-766X
通讯作者：
Cao, Deliang
作者机构：
[Wen, Gebo; Cao, Renxian; Zhong, Jing; Liu, Jianghua; Zu, Xuyu; Zhang, Qinghai] Univ S China, Affiliated Hosp 1, Clin Res Inst, Hengyang 421001, Hunan, Peoples R China.;[Cao, Deliang] So Illinois Univ, Sch Med, Simmons Canc Inst, Dept Med Microbiol Immunol & Cell Biol, Springfield, IL 62794 USA.;[Cao, Deliang] So Illinois Univ, Sch Med, Simmons Canc Inst, Dept Med Microbiol Immunol & Cell Biol, 913 N Rutledge St, Springfield, IL 62794 USA.
通讯机构：
[Cao, Deliang] S;So Illinois Univ, Sch Med, Simmons Canc Inst, Dept Med Microbiol Immunol & Cell Biol, 913 N Rutledge St, Springfield, IL 62794 USA.
关键词：
Breast cancer;Cancer therapy;Epithelial-mesenchymal transition;TGF-β signaling;Tumorigenesis
摘要：
Transforming growth factor-β (TGF-β) is a ubiquitous cytokine playing an essential role in cell proliferation, differentiation, apoptosis, adhesion and invasion, as well as in cellular microenvironment. In malignant diseases, TGF-β signaling features a growth inhibitory effect at an early stage but aggressive oncogenic activity at the advanced malignant state. Here, we update the current understanding of TGF-β signaling in cancer development and progression with a focus on breast cancer. We also review the current approaches of TGF-β signaling-targeted therapeutics for human malignancies. © Springer-Verlag 2011.
语种：
英文
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构建面向乡镇的全科医学人才培养体系探析
作者：
张新华;文格波;田英;唐志晗
期刊：
中华医学教育杂志 ,2012年32(2):173-174+231 ISSN：1673-677X
作者机构：
南华大学医学院临床技能教学中心,衡阳,421001;南华大学医学院,衡阳,421001;[唐志晗; 文格波; 张新华; 田英] 南华大学
关键词：
全科医学;乡镇;人才培养体系
摘要：
培养乡镇全科医学人才,需要政府部门、高等学校和基地医院三个主体形成合力,主要采用学历教育、规范化培训、转岗培训、进修学习四种形式,实行临床医学专业全科医学方向人才"5+3"培养模式和在职人员"转身+提升"模式。把握层次定位、功能定位和岗位定位,以临床医学为主干,增加预防医学知识与技能,调整基础医学知识结构。加强实践能力培养,注重人文素质教育。贯彻国家宏观政策,在基地、教材、师资、制度方面采取积极措施。
语种：
中文
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临床技能学教学中融入人文素质教育的探讨
作者：
邹飞燕;唐志晗;张新华;刘江华;田英;...
期刊：
医学教育研究与实践 ,2012年20(1):143-145 ISSN：2096-3181
作者机构：
南华大学医学院,湖南衡阳,421001
关键词：
医学生;人文素质教育;临床技能学
摘要：
加强医学生人文素质教育是当前高等医学教育面临的一项重要任务。本文分析了我国高校临床技能学教学现状,提出了从临床技能学实践教学内容、师资队伍和考核评估等多种途径加强人文素质教育,不但可以提高医学生的学习效果,也是培养高素质医学人才的迫切需求。
语种：
中文
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医学生人文素质教育“五个一”体系的构建与实践
作者：
文格波;张新华;唐志晗;吴移谋;姜志胜
期刊：
中国高等医学教育 ,2012年(5):35-36 ISSN：1002-1701
作者机构：
南华大学医学院,湖南衡阳,421001;[唐志晗; 文格波; 吴移谋; 姜志胜; 张新华] 南华大学
关键词：
医学生;人文素质教育;体系
摘要：
按照知识、技能和素质协调发展的目标要求，探索提出医学生人文素质教育目标定位，完善优化医学生人文素质教育培养方案，整合出版人文素质教育必要教材，配强用活人文素质教育教学力量，营造和建设人文素质教育环境，由此构建医学生人文素质教育“五个一”体系，为培养高素质医学人才提供有力保障。
语种：
中文
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Sudden onset agranulocytosis and hepatotoxicity after taking methimazole
作者：
Yang, Jing;Zhong, Jing;Zhou, Ling-Zhi;Hong, Tao;Xiao, Xin-Hua;...
期刊：
Internal Medicine ,2012年51(16):2189-2192 ISSN：0918-2918
通讯作者：
Wen, Ge-Bo
作者机构：
[Hong, Tao; Wen, Ge-Bo; Xiao, Xin-Hua; Yang, Jing] Univ S China, Affiliated Hosp 1, Dept Endocrinol & Metab, Hengyang, Peoples R China.;[Zhong, Jing; Wen, Ge-Bo; Zhou, Ling-Zhi] Univ S China, Affiliated Hosp 1, Clin Med Res Inst, Hengyang, Peoples R China.;[Yang, Jing] Univ S China, Dept Pathophysiol, Hengyang, Peoples R China.
通讯机构：
[Wen, Ge-Bo] U;Univ S China, Affiliated Hosp 1, Dept Endocrinol & Metab, Hengyang, Peoples R China.
关键词：
agranulocytosis;hepatotoxicity;methimazole;hyperthyroidism
摘要：
Agranulocytosis is a rare adverse effect of methimazole. The usual duration of treatment prior to the onset of agranulocytosis is approximately 1 to 4 months, and can be as long as 1 year. Agranulocytosis together with hepatotoxicity is an extremely rare idiosyncratic side effect of methimazole treatment. We present an unprecedented case of a Grave's disease patient who showed a strong reaction to methimazole with obvious agranulocytosis and hepatotoxicity which developed only six days after administration. This case, along with a literature review, is offered with the aim to increase the awareness of physicians of sudden onset agranulocytosis and hepatotoxicity from methimazole.
语种：
英文
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MiRNA-21 reverses high glucose and high insulin induced insulin resistance in 3T3-L1 adipocytes through targeting phosphatase and tensin homologue
作者：
Ling, H. -y.;Hu, B.;Hu, X. -b.;Zhong, J.;Feng, S. -d.;...
期刊：
EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES ,2012年120(9):553-559 ISSN：0947-7349
通讯作者：
Liao, D. -f.
作者机构：
[Ling, H. -y.; Hu, B.] Univ S China, Sch Med, Dept Physiol, Hengyang, Peoples R China.;[Liao, D. -f.] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410208, Hunan, Peoples R China.;[Ling, H. -y.] Univ S China, Ctr Basic Med Postdoctoral Studies, Hengyang, Peoples R China.;[Hu, X. -b.] Univ S China, Sch Life Sci & Technol, Dept Biochem & Mol Biol, Hengyang, Peoples R China.;[Wen, G. -b.; Zhong, J.] Univ S China, Affiliated Hosp 1, Inst Clin Res, Hengyang, Peoples R China.
通讯机构：
[Liao, D. -f.] H;Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410208, Hunan, Peoples R China.
关键词：
miR-21;insulin resistance;PTEN;AKT
摘要：
Aims/hypothesis: Our previous study showed there was a change of microRNA (miRNA) expression profile, and miR-21 was significantly down regulated in insulin-resistant adipocytes (IR-adipocytes). Phosphatase and tensin homologs deleted on chromosome 10 (PTEN), a negative regulator of the phosphatidylinositol 3-kinase (PI3K)/AKT pathway, was identified to be a target gene of miR-21, which suggested miR-21 might be associated with insulin resistance (IR) or diabetes. However, it is not known whether miR-21 play any role in the development of IR in 3T3-L1 adipocytes. Methods: Normal adipocytes and adipocytes transfected with pre-miR-21(pmiR-21) or negative control (pNeg) were treated with high glucose and high insulin for 24h, insulin-stimulated glucose uptake was determined by 2-Deoxyglucose transport assay, miR-21 expression level was measured by using quantitative real-time RT-PCR (qRT-PCR). The protein expression levels of PTEN, Akt, phospho-Akt (Ser473), IRβ, GSK3β, phospho-GSK3β (Ser9) and GLUT4 were detected by western blotting assay. Results: We further confirmed that miR-21 was down regulated in IR-adipocytes by qRT-PCR. Over-expression of miR-21 significantly increased insulin-induced glucose uptake and decreased PTEN protein expression, while it had no significant effect on PTEN mRNA expression in IR-adipocytes. Moreover, over-expressing miR-21 significantly increased insulin-induced phosphorylation of AKT (Ser473), GSK3β (Ser9) and the translocation of glucose transporter 4 (GLUT4) in IR-adipocytes. Conclusions: In this study, our data demonstrate that miR-21 reverses high glucose and high insulin induced IR in 3T3-L1 adipocytes, possibly through modulating the PTEN-AKT pathway, and miR-21 may be a new therapeutic target for metabolic diseases such as T2DM and obesity. © Georg Thieme Verlag KG Stuttgart · New York.
语种：
英文
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MicroRNA-375 promotes 3T3-L1 adipocyte differentiation through modulation of extracellular signal-regulated kinase signalling
作者：
Ling, Hong-Yan;Wen, Ge-Bo;Feng, Shui-Dong;Tuo, Qin-Hui;Ou, He-Sheng;...
期刊：
Clinical and Experimental Pharmacology and Physiology ,2011年38(4):239-246 ISSN：0305-1870
通讯作者：
Zhang, Liang
作者机构：
[Ling, Hong-Yan] Univ S China, Sch Med, Dept Physiol, Hengyang, Peoples R China.;[Wen, Ge-Bo; Ling, Hong-Yan] Univ S China, Affiliated Hosp 1, Inst Clin Res, Hengyang, Peoples R China.;[Feng, Shui-Dong] Univ S China, Sch Publ Hlth, Dept Epidemiol, Hengyang, Peoples R China.;[Gao, Zhi-Ping; Zhu, Bing-Yang; Ou, He-Sheng; Tuo, Qin-Hui; Liao, Duan-Fang] Univ S China, Inst Pharm & Pharmacol, Key Lab Pharmacoprote Hunan Prov, Hengyang, Peoples R China.;[Liao, Duan-Fang] Hunan Univ Chinese Med, Sch Pharm, Dept Tradit Chinese Diagnost, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhang, Liang] P;Palmer Coll Chiropract Florida, Palmer Ctr Chiropract Res, Lab Cell & Mol Biol, 4705 S Clyde Morris Blvd, Port Orange, FL 32129 USA.
关键词：
3T3-L1 adipocytes;Differentiation;Extracellular signal-regulated kinases 1/2;MicroRNA-375;Obesity
摘要：
1. Adipocyte hypertrophy and hyperplasia are important processes in the development of obesity. To understand obesity and its associated diseases, it is important to elucidate the molecular mechanisms governing adipogenesis. MicroRNA-375 has been shown to inhibit differentiation of neurites, and participate in the regulation of insulin secretion and blood homeostasis. However, it is unknown whether miR-375 plays a role in adipocyte differentiation. 2. To investigate the role of miR-375 in adipocyte differentiation, we compared the miR-375 expression level between 3T3-L1 pre-adipocytes and adipocytes using miRNA microarray and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analysis. Furthermore, we evaluated the effects of overexpression or inhibition of miR-375 on 3T3-L1 adipocyte differentiation. 3. In the present study, we found that miR-375 expression was increased after induction of adipogenic differentiation. Overexpression of miR-375 enhanced 3T3-L1 adipocyte differentiation, as evidenced by its ability to increase mRNA levels of both CCAAT/enhancer binding protein-α (C/EBPα) and peroxisome proliferator-activated receptor-γ (PPARγ2), and induction of adipocyte fatty acid-binding protein (aP2) and triglyceride (TG) accumulation. Furthermore, we found overexpression of miR-375 suppressed phosphorylation levels of extracellular signal-regulated kinases 1/2 (ERK1/2). In contrast, anti-miR-375 increased ERK1/2 phosphorylation levels and inhibited mRNA expression of C/EBPα, PPARγ2 and aP2 in 3T3-L1 adipocyte, accompanied by decreased adipocyte differentiation. 4. Taken together, these data suggest that miR-375 promotes 3T3-L1 adipocyte differentiation, possibly through modulating the ERK-PPARγ2-aP2 pathway. © 2011 The Authors. Clinical and Experimental Pharmacology and Physiology © 2011 Blackwell Publishing Asia Pty Ltd.
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英文
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Tristetraprolin-dependent post-transcriptional regulation of inflammatory cytokine mRNA expression by apolipoprotein A-I: Role of ATP-binding membrane cassette transporter a1 and signal transducer and activator of transcription 3
作者：
Yin, Kai;Deng, Xiang;Mo, Zhong-Cheng;Zhao, Guo-Jun;Jiang, Jin;...
期刊：
Journal of Biological Chemistry ,2011年286(16):13834-13845 ISSN：0021-9258
通讯作者：
Tang, Chao-Ke
作者机构：
[Zhao, Guo-Jun; Tan, Chun-Zhi; Tang, Chao-Ke; Wen, Ge-Bo; Yin, Kai; Mo, Zhong-Cheng; Jiang, Jin; Cui, Li-Bao] Univ S China, Affiliated Hosp 1, Key Lab Atherosclerol Hunan Prov, Life Sci Res Ctr,Inst Cardiovasc Res, Hengyang 421001, Peoples R China.;[Wen, Ge-Bo; Yin, Kai] Univ S China, Affiliated Hosp 1, Dept Diagnost, Coll Med, Hengyang 421001, Peoples R China.;[Wen, Ge-Bo; Deng, Xiang] Univ S China, Affiliated Hosp 1, Dept Endocrinol & Metab, Hengyang 421001, Peoples R China.;[Fu, Yuchang] Univ Alabama, Dept Nutr Sci, Birmingham, AL 35294 USA.
通讯机构：
[Tang, Chao-Ke] U;Univ S China, Affiliated Hosp 1, Key Lab Atherosclerol Hunan Prov, Life Sci Res Ctr,Inst Cardiovasc Res, Hengyang 421001, Peoples R China.
摘要：
Atherosclerosis is an inflammatory disease characterized by the accumulation of macrophages in the arterial intima. The activated macrophages secreted more pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, which promote the development of the disease. Apolipoprotein A-I (apoA-I), the major component of high density lipoprotein, is involved in reverse cholesterol transport of lipid metabolism. Recently, it has been found that apoA-I suppresses inflammation via repression of inflammatory cytokine expression;the mechanisms of the apoA-I-suppressive action, however, are not yet well characterized. In this study, we have for the first time found that apoA-I suppresses the expression of some inflammatory cytokines induced by lipopolysaccharide via a specific post-transcriptional regulation process, namely mRNA destabilization, in macrophages. Our further studies have also shown that AU-rich elements in the 3′-untranslated region of TNF-αmRNA are responsive to the apoA-I-mediated mRNA destabilization. The apoA-I-induced inflammatory cytokine mRNA destabilization was associated with increased expression of mRNA-destabilizing protein tristetraprolin through aJAK2/STAT3 signaling pathway-dependent manner. When blocking interaction of apoA-Iwith ATP-binding membrane cassette transporter A1 (ABCA1), a major receptor for apoA-Iin macrophages, it would almost totally abolish the effect of apoA-I on tristetraprolin expression. These results present not only a novel mechanism for the apoA-I-mediated inflammation suppression in macrophages but also provide new insights for developing strategies for modulating vascular inflammation and atherosclerosis. ©2011 by The American Society for Biochemistry and Molecular Biology, Inc.
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浅谈基础医学课程蕴含的人文素养
作者：
邹飞燕;唐志晗;王汉群;张新华;文格波
期刊：
教育教学论坛 ,2011年(31):217-219 ISSN：1674-9324
作者机构：
南华大学医学院,湖南衡阳,421001
关键词：
人文素质;基础医学课程;医学生
摘要：
加强医学生的人文素质修养已成为高等医学院校教育的重要内容。本文对基础医学课程中蕴含的人文素养进行了提炼与归纳，旨在加强医学生的人文素质教育，培养既具备较高的专业技术水平、又具有高尚的职业道德和健康的心理素质的优秀医学人才。
语种：
中文
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基于国家医学教育标准的临床教学质量强化对策探讨
作者：
张新华;唐志晗;张天成;文格波;吴移谋;...
期刊：
中华医学教育杂志 ,2011年31(4):502-504 ISSN：1673-677X
作者机构：
南华大学医学院临床技能教学中心,衡阳,421001;南华大学医学院,衡阳,421001
关键词：
医学教育;临床教学质量;对策
摘要：
提高教学质量是高等教育的永恒主题。确保临床教学质量对于培养合格的临床医学专业人才至关重要。以国家医学教育标准为指导，借鉴教学质量保障与监控体系，从调整课程设置、优化教学模式、培训提高临床师资、改善临床教学条件和建立针对性工作制度等方面提出质量保障的强化对策；从适时完善质量标准、多渠道收集整理教学信息、规范开展教与学质量评价、多途径实施教学信息反馈与调控、坚持“三个结合”的实施策略等方面提出质量监控的强化对策。r单自毫：=l’r矗越血‘古tI肛r卜血0曲；F}旦．寸46盘
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中文
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临床医学专业方向建设探讨——以南华大学为例
作者：
张新华;唐志晗;文格波;吴移谋;姜志胜;...
期刊：
南方医学教育 ,2011年(2):3-4+22
作者机构：
南华大学医学院
关键词：
医学教育;临床医学专业;建设
摘要：
专业方向的课程设置必须服从于人才培养目标,体现于人才培养方案,在提升人文、夯实基础、强化技能的同时,更加注重促进专长,对各方向的课程设置做出针对性和特色性安排,以利于学生根据不同就业流向及职业取向选择相应课程。
语种：
中文
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小鼠间充质干细胞诱导分化成脂肪细胞miRNA表达的变化
作者：
凌宏艳;文格波;胡弼;奉水东;张恺芳;...
期刊：
中国应用生理学杂志 ,2011年27(4):391-395 ISSN：1000-6834
通讯作者：
Ling, H.Y.
作者机构：
南华大学生理学教研室,湖南衡阳,421001;南华大学附一医院临床研究所,湖南衡阳,421001;南华大学流行病学教研室,湖南衡阳,421001;湖南中医药大学药学院,长沙,410208;[杨丝丝; 奉水东; 凌宏艳; 张恺芳; 胡弼; 尹蔚兰; 何剑琴] 南华大学
关键词：
miRNA表达;骨髓间充质干细胞;分化;脂肪细胞
摘要：
目的：探讨小鼠间充质干细胞（MSCs）定向诱导分化成脂肪细胞微小RNA（miRNA）表达的变化，为进一步研究miRNA调控MSCs向脂肪细胞分化的分子机制奠定基础。方法：采用全骨髓体外分离结合差速贴壁法纯化扩增C57BL／6小鼠MSCs，形态学观察细胞生长情况，并用免疫组化方法鉴定细胞表面抗原CD29、CIM4和CD34的表达。脂肪细胞分化诱导剂诱导MSCs分化为脂肪细胞，利用油红O染色，判断MSCs成脂分化情况。运用rrfiRNA芯片技术检测MSC8和脂肪细胞中差异表达的miRNA。结果：①倒置显微镜下观察，传5代后可获得均一性较高的MSCs；免疫组化显示90％以上的骨髓间质干细胞CD29、CD44阳性，CD34阴性。MSCs经脂肪诱导剂诱导后，胞内大量脂滴形成，油红O染色阳性；②基因微阵列分析表明，小鼠MSCs分化成脂肪细胞差异表达的miRNA共75个，其中20个表达上调、55个表达下调。结论：MSCs分化成脂肪细胞存在miRNA表达的变化，某些miRNA很可能具有重要的调控MSCs成脂分化的作用。
语种：
中文
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临床医学专业人才培养体系优化路径选择与实践
作者：
张新华;唐志晗;文格波;吴移谋;姜志胜;...
期刊：
医学教育研究与实践 ,2011年19(4):672-674 ISSN：2096-3181
作者机构：
南华大学医学院,湖南衡阳,421001;[唐志晗; 文格波; 吴移谋; 姜志胜; 张新华; 田英] 南华大学
关键词：
医学教育;临床医学专业;培养体系;优化路径
摘要：
分析了我国临床医学专业教育面临着新的情况,即人文素质教育总体水平有所下降,医学知识传授和临床技能培养质量受到教育资源适应性短缺的影响,医药卫生体制深化改革对医学专业人才服务重点和培养目标提出了新的要求,医学教育标准制定实施为临床医学专业人才培养提供了权威指导;提出了培养体系的优化路径,既要优化培养目标、培养方案、教学模式和方向设置,又要强化人文素质教育、基础实验教学、临床技能培养、师资队伍与依托学科建设;总结了南华大学临床医学专业优化改革的初步成效。
语种：
中文
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