作者机构:
[余益本] Department of Gerontology, Dongfeng Hospital, Hubei University of Medicine, Shiyan 442000;[余益本] Laboratory of Pathogenic Biology, National Center of Clinical Medicine Research, University of South China, Hengyang 421001, China;[吴移谋] Laboratory of Pathogenic Biology, University of South China, Hengyang 421001, China;[文格波] National Center of Clinical Medicine Research, University of South China, Hengyang 421001, China;[杨文琼] Department of Neurology, Dongfeng Hospital, Hubei University of Medicine, Shiyan 442000, China
摘要:
The interaction of blood glucose with heme proteins plays a key role in inducing diabetes, a serious disease threatening human health. In this study, we investigated the non-covalent interaction between glucose and myoglobin (Mb), both theoretically and experimentally, using molecular dynamics (MD) simulation combined with spectroscopic studies. It revealed that glucoses can occupy the side pocket of Mb, and bind closely to one of the xenon cavities in Mb, by hydrogen bonding interactions with two propionate groups of heme as well as surrounding amino acids. These interactions alter the conformation of the heme active site slightly and lead to an enhanced peroxidase activity of Mb, as determined by kinetic studies. This study provides general information for glucose-heme proteins interactions, and also for blood glucose-protein interactions for patients with diabetes.
摘要:
Glucocorticoids (GCs) are well known to induce fat distribution; which is consistent with the central adiposity phenotype seen in Cushing's syndrome. GCs have been proposed to be both adipogenic and lipolytic in action within adipose tissues. Different adipogenic and lipolytic effects between subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) are likely to play a role in GCs induced fat differential distribution. Wnt/beta-catenin signaling pathway is one of the most important regulators in adipogenesis. Adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) are the major lipases contributing to lipolysis. In the present study, we measured fat depot masses and the expression of Wnt/beta-catenin signaling pathway and lipolytic enzymes of female Sprague-Dawley rats treated with or without methylprednisolone. We assessed the roles of Wnt/beta-catenin signaling pathway and lipolytic enzymes in fat differential distribution between SAT and VAT. Our data suggested that methylprednisolone could inhibit Wnt/beta-catenin signaling pathway in SAT and VAT, increase the expression of ATGL and HSL in SAT, and decrease the expression of ATGL and HSL in VAT. The differential expression of lipolysis enzymes induced by methylprednisolone between SAT and VAT might play a crucial role in fat distribution. Those findings would offer novel insights into the mechanisms of GCs induced fat distribution. (C) 2014 Elsevier Inc. All rights reserved.
作者机构:
[Liu Chang-hui; Wu Jie; Yuan Cong; Zou Jin; Xu Ye-ping] Univ South China, Affiliated Hosp 1, Dept Cardiol, Hengyang, Peoples R China.;[Yuan Cong; Wen Ge-bo; Jiang Zhi-sheng] Univ South China, Inst Cardiovasc Dis, Hengyang, Peoples R China.;[Yuan Cong; Wen Ge-bo; Jiang Zhi-sheng] Univ South China, Key Lab Arteriosclerol, Hengyang, Peoples R China.
会议名称:
第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复 会议(The 24th Great Wall International Congress of Cardiology & Asia Pacific Heart Congress & International Congress of Cardiovascular Prevention and Rehabilitation 2013)
会议时间:
2013-10-10
会议地点:
北京
会议主办单位:
中华医学会;长城国际心脏病学会议组委会
会议论文集名称:
第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复 会议(The 24th Great Wall International Congress of Cardiology & Asia Pacific Heart Congress & International Congress of Cardiovascular Prevention and Rehabilitation 2
摘要:
The official journal of Sigma Theta Tau International, Honor Society of Nursing, Inc., Image is intended to provide a forum for the publication of superior nursing thought in the general areas of clinical scholarship and policy. Research reports, reviews of literature, and discursive pieces are regularly published.Coverage in the Journals@Ovid database begins with the January 1995 issue.
作者机构:
[Wen, Gebo; Zhong, Jing; Cao, Renxian; Tan, Jingjing; Wu, Ying; Liu, Jianghua; Zu, Xuyu; Zhang, Qinghai] Univ South China, Inst Clin Med, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.;[Luo, Dixian] First Peoples Hosp Chenzhou, Inst Translat Med, Chenzhou 423000, Hunan, Peoples R China.;[Luo, Dixian] First Peoples Hosp Chenzhou, Dept Lab Med, Chenzhou 423000, Hunan, Peoples R China.;[Cao, Deliang] So Illinois Univ, Sch Med, Simmons Canc Inst, Dept Microbiol Immunol & Cell Biol, Springfield, IL 62794 USA.
通讯机构:
[Cao, Renxian] U;Univ South China, Inst Clin Med, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.
关键词:
acetyl-CoA carboxylase;fatty acid biosynthesis;ACC chemical genetics;ACC inhibitors;cancer therapy
摘要:
Chemical genetic studies on acetyl-CoA carboxylases (ACCs), rate-limiting enzymes in long chain fatty acid biosynthesis, have greatly advanced the understanding of their biochemistry and molecular biology and promoted the use of ACCs as targets for herbicides in agriculture and for development of drugs for diabetes, obesity and cancers. In mammals, ACCs have both biotin carboxylase (BC) and carboxyltransferase (CT) activity, catalyzing carboxylation of acetyl-CoA to malonyl-CoA. Several classes of small chemicals modulate ACC activity, including cellular metabolites, natural compounds, and chemically synthesized products. This article reviews chemical genetic studies of ACCs and the use of ACCs for targeted therapy of cancers.