作者：
Jun He;Shan Yu;Shaojian Wu;Yanhua Zeng;Xiaoxing You;...
作者机构：
[Jun He; Shan Yu; Shaojian Wu] Department of Clinical Laboratory, Nanhua Affiliated Hospital, University of South China, Hengyang 421001, China;[Yanhua Zeng; Xiaoxing You; Yimou Wu] Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, China
会议名称：
The 6th Meeting of the Asian Organization for Mycoplasmology (AOM)The 7th Meeting of the Chinese Society for Mycoplasmology (CSM)(第七届全国支原体学术会议暨第六届亚洲支原体学术会议)
会议时间：
2014-8-22
会议地点：
张家界
会议主办单位：
中华医学会微生物与免疫学分会;亚洲支原体学组织
会议论文集名称：
The 6th Meeting of the Asian Organization for Mycoplasmology (AOM)The 7th Meeting of the Chinese Society for Mycoplasmology (CSM)(第七届全国支原体学术会议暨第六届亚洲支原体学术会议)论文集
摘要：
Objective Two primary enzymes GlpK and GlpO of glycerol metabolism from M.genitalium were chosen.This study intends to study their localization, enzyme activity and abilities to involve in the cytotoxicity of M.genitalium on host cell.Methods Prokaryotic expression vector pGEX6p-1/G1pK was constructured and GST-GlpK fusion protein was expressed.BALB/c mice were immuned by purified protein and purified antiserum titers were detected.
期刊：
Infection and Immunity,2014年82(12):5327-5335 ISSN：0019-9567
通讯作者：
Zhong, Guangming
作者机构：
[Liu, Yuanjun; Yang, Zhangsheng; Baseman, Joel; Huang, Yumeng; Gong, Siqi; Zhong, Guangming] Univ Texas Hlth Sci Ctr San Antonio, Dept Microbiol & Immunol, San Antonio, TX 78229 USA.;[Liu, Quanzhong; Hou, Shuping] Tianjin Med Univ Gen Hosp, Dept Dermatovenereol, Tianjin, Peoples R China.;[Sun, Yina] Tianjin Med Univ, Hosp & Tianjin Inst Endocrinol, Key Lab Hormones & Dev Minist Health, Metab Dis, Tianjin, Peoples R China.;[Li, Zhongyu; Wu, Yimou; Chen, Chaoqun] Univ South China, Dept Microbiol & Immunol, Hengyang, Hunan, Peoples R China.
通讯机构：
[Zhong, Guangming] U;Univ Texas Hlth Sci Ctr San Antonio, Dept Microbiol & Immunol, San Antonio, TX 78229 USA.
摘要：
Hydrosalpinx induction in mice by Chlamydia muridarum infection, a model that has been used to study C. trachomatis pathogenesis in women, is known to depend on the cryptic plasmid that encodes eight genes designated pgp1 to pgp8. To identify the plasmid-encoded pathogenic determinants, we evaluated C. muridarum transformants deficient in the plasmid-borne gene pgp3, -4, or -7 for induction of hydrosalpinx. C. muridarum transformants with an in-frame deletion of either pgp3 or -4 but not -7 failed to induce hydrosalpinx. The deletion mutant phenotype was reproduced by using transformants with premature termination codon insertions in the corresponding pgp genes (to minimize polar effects inherent in the deletion mutants). Pgp4 is known to regulate pgp3 expression, while lack of Pgp3 does not significantly affect Pgp4 function. Thus, we conclude that Pgp3 is an effector virulence factor and that lack of Pgp3 may be responsible for the attenuation in C. muridarum pathogenicity described above. This attenuated pathogenicity was further correlated with a rapid decrease in chlamydial survival in the lower genital tract and reduced ascension to the upper genital tract in mice infected with C. muridarum deficient in Pgp3 but not Pgp7. The Pgp3-deficient C. muridarum organisms were also less invasive when delivered directly to the oviduct on day 7 after inoculation. These observations demonstrate that plasmid-encoded Pgp3 is required for C. muridarum survival in the mouse genital tract and represents a major virulence factor in C. muridarum pathogenesis in mice.
摘要：
Chlamydophila psittaci, an obligate intracellular pathogen, has been associated with psittacosis.Also, C.psittaci is a zoonotic agent.CPSIT_0846, a putative transmembrane head (TMH) family protein, was detected in the inclusion membrane during C.psittaci infection with antibodies raised with CPSIT_0846 fusion proteins by immunofluorescence assay.In the time-course monitoring, RT-PCR and Western blot revealed that it could be present as early as 2 h post infection (hpi), while it was visualized in inclusion membrane at 18 hpi in immunofluorescence assay, and remained in inclusion membrane throughout the growth cycle.CPSIT_0846 had indicated no significant orthologues with Chlamydia trachomatis and Chlamydophila pneumoniae, and in our experiment, recombinated CPSIT_0846 protein could not react with the serum of C.trachomatis and C.pneumoniae but could strongly recognize with the serum of C.psittaci, which revealed CPSIT_0846 is C.psittaci specific, so it could be used to diagnose human chlamydia disease cause by C.psittaci.Moreover, CPSIT_0846 could induce the expression of the inflammatory cytokines interleukin-1 Beta (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-oα) in THP-1 cells, which might be associated with chlamydia-induced inflammatory pathologies.
作者机构：
[Xilin Xiao; Bo He; Lifu Liao; Shuqin Gao] College of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China;[Yanhua Zeng; Yimou Wu; Youcong Zhu] Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, China
会议名称：
The 6th Meeting of the Asian Organization for Mycoplasmology (AOM)The 7th Meeting of the Chinese Society for Mycoplasmology (CSM)(第七届全国支原体学术会议暨第六届亚洲支原体学术会议)
会议时间：
2014-8-22
会议地点：
张家界
会议主办单位：
中华医学会微生物与免疫学分会;亚洲支原体学组织
会议论文集名称：
The 6th Meeting of the Asian Organization for Mycoplasmology (AOM)The 7th Meeting of the Chinese Society for Mycoplasmology (CSM)(第七届全国支原体学术会议暨第六届亚洲支原体学术会议)论文集
摘要：
Magnetoelastic biosensors are wireless passive sensors and develop rapidly in recent years.Magnetoelastic materials are made of amorphous ferromagnetic alloys composed of iron, nickel,molybdenum and boron.
作者机构：
Department of Clinical Laboratory, Changsha Central Hospital, Changsha 410004, China;[游晓星; Zeng Y.; 朱翠明; 刘良专; 吴移谋; 蒋传好] Institute of Pathogenic Biology, Medical College of University of South China, Hengyang 421001, China;[马小华] Department of Clinical Laboratory, Changsha Central Hospital, Changsha 410004, China, Institute of Pathogenic Biology, Medical College of University of South China, Hengyang 421001, China
通讯机构：
[Wu, Y.] I;Institute of Pathogenic Biology, Medical College of University of South China, Hengyang 421001, China
摘要：
Glycogen has been localized both inside and outside Chlamydia trachomatis organisms. We now report that C. trachomatis glycogen synthase (GlgA) was detected in both chlamydial organism-associated and -free forms. The organism-free GlgA molecules were localized both in the lumen of chlamydial inclusions and in the cytosol of host cells. The cytosolic GlgA displayed a distribution pattern similar to that of a known C. trachomatis-secreted protease, CPAF. The detection of GlgA was specific since the anti-GlgA antibody labeling was only removed by preabsorption with GlgA but not CPAF fusion proteins. GlgA was detectable at 12h and its localization into host cell cytosol only became apparent at 24h after infection. The cytosolic localization of GlgA was conserved among all C. trachomatis serovars. However, the significance of the GlgA secretion into host cell cytoplasm remains unclear since, while expression of chlamydial GlgA in HeLa cells increased glycogen stores, it did not affect a subsequent infection with C. trachomatis. Similar to several other C. trachomatis-secreted proteins, GlgA is immunogenic in women urogenitally infected with C. trachomatis, suggesting that GlgA is expressed and may be secreted into host cell cytosol during C. trachomatis infection in humans. These findings have provided important information for further understanding C. trachomatis pathogenic mechanisms.
