作者机构：
[李文彬; 吴移谋] Institution of Pathogenic Biology, Medical College, University of South China, Hengyang, 421001, China;[徐冬蕾; 赵秀芹; 刘海灿; 万康林] National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China;Department of Laboratory Medicine, Shanghai East Hospital, Tongji University, Shanghai, 200123, China;[纪凌云] National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China, Department of Laboratory Medicine, Shanghai East Hospital, Tongji University, Shanghai, 200123, China
通讯机构：
[Wan, K.] N;National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
期刊：
Pathogens and Disease,2018年76(2) ISSN：2049-632X
通讯作者：
Wu, Yimou
作者机构：
[Wu, Yimou; Zhao, Feijun; Tan, Manyi; Zheng, Kang; Xu, Man; Zeng, Tiebing; Chen, Qian] Univ South China, Med Coll, Pathogen Biol Inst, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.;[Xie, Yafeng; Xiao, Yongjian] Univ South China, Dept Clin Lab, Affiliated Hosp 2, Hengyang 421001, Hunan, Peoples R China.;[Jiang, Chuanhao] Cent S Univ, Xiangya Hosp 2, Dept Lab Med, Changsha 410011, Hunan, Peoples R China.
通讯机构：
[Wu, Yimou] U;Univ South China, Med Coll, Pathogen Biol Inst, 28 West Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
"Treponema pallidum";"FlaB protein fragments";serodiagnosis;syphilis
摘要：
Flagellin is a classical pathogen-associated molecular pattern that can evoke a robust immune response. We have demonstrated previously that three full-length flagellins of Treponema pallidum, namely FlaB1, FlaB2 and FlaB3, did have diagnostic value in the serodiagnosis of syphilis. Here, we selected and constructed three recombinant fragments of each complete FlaB, both the conserved N-terminal and the C-terminal region, and the middle variable part, with the goal of exploring fragments unique to Treponema pallidum for use as antigen targets in a fragment-based serological test. The diagnostic performance of fragments was evaluated using different panels of serum specimens (= 332) by indirect IgG enzyme-linked immunosorbent assay. The data showed that all the conserved fragments exhibited excellent sensitivities (91.1–95.0%) but poor specificities (64.1–78.4%), while the three middle regions demonstrated higher sensitivities and specificities for detecting IgG antibody, with 92.7% and 96.1% for FlaB1M (‘B1M’), 91.6% and 94.8% for B2M, and 95.0% and 100% for B3M, respectively. In comparison, the sensitivity and specificity of Architect Syphilis TP was found to be 95.5% and 94.8%, respectively. These findings revealed that the middle portion of each FlaB had epitopes specific for Treponema pallidum and identified B3M as a promising candidate antigen for the serodiagnosis of syphilis.
期刊：
MOLECULAR MEDICINE REPORTS,2018年17(3):4155-4155 ISSN：1791-2997
作者机构：
[Tan, Tianping; Ye, Zhufeng; Liu, Xinghui; He, Jun; Liu, Mihua] Department of Clinical Laboratory, Nanhua Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China;[Wu, Yimou; You, Xiaoxing; Zeng, Yanhua] Pathogenic Biology Institute, University of South China, Hengyang, Hunan 421001, P.R. China
摘要：
Following the publication of this review, an interested reader alerted us to the fact that a couple of figures had been reproduced from a pair of previous publications without proper acknowledgement of the original source/authors. Figs. 2 and 3, as featured in our review, had originally appeared (with only minor modifications) as Figs. 2 and 4, respectively, in the following articles: Rottem S: Interaction of mycoplasmas with host cells. Physiol Rev 83: 417-32, 2003; and Pilo P, Vilei EM, Peterhans E, Bonvin-Klotz L, Stoffel MH, Dobbelaere D and Frey J: A metabolic enzyme as a primary virulence factor of Mycoplasma mycoides subsp. mycoides small colony. J Bacteriol 187: 6824-6831, 2005. Permission to publish these figures was sought retrospectively from the publishers [The American Physiological Society (Fig. 2) and The American Society of Microbiology (Fig 3)]. Subsequently, Figs. 2 and 3 are reprinted in this Corrigendum, together with strap-lines that properly acknowledge the source articles. In addition, we omitted to explain that the glycerol metabolism causing injury in host cells refers to Mycoplasma mycoides subsp. mycoides. Consequently, this information has also been inserted into the corrected legend for Fig. 3 (opposite), with a pair of supporting references. We profusely apologize to the authors of the previous publications (Dr Joachim Frey and colleagues) for our having failed to include a proper acknowledgement of their figure, or to have credited their work appropriately. [the original article was published in the Molecular Medicine Reports 14: 4030-4036, 2016; DOI: 10.3892/mmr.2016.5765].
摘要：
'Obesity Facts' publishes articles covering all aspects of obesity, in particular epidemiology, etiology and pathogenesis, treatment, and the prevention of adiposity. As obesity is related to many disease processes, the journal is also dedicated to all topics pertaining to comorbidity and covers psychological and sociocultural aspects as well as influences of nutrition and exercise on body weight. The editors carefully select papers to present only the most recent findings in clinical practice and research. All professionals concerned with obesity issues will find this journal a most valuable update to keep them abreast of the latest scientific developments.Special sections comprising a variety of subspecialties reinforce the journal's value as an exhaustive record of recent progress for all internists, gastroenterologists, endocrinologists, pediatricians, dieticians, nutritionists, bariatric surgeons, psychologists and psychiatrists, occupational health practitioners, sports medicine specialists, ecotrophologists, sociologists, and biologists as well as prevention and public health researchers. In addition, 'Obesity Facts' serves as an ideal information tool for the members of the pharmaceutical and food industry as well as those active in nutritional research and medicine.
摘要：
The obligate intracellular bacterium Chlamydia psittaci is the causative agent of psittacosis in birds and humans. The capability of this zoonotic pathogen to develop a persistent phase may serve a role in the chronicity of infections, in addition to the failure of antibiotic therapy or immunoprophylaxis. In the present study, a C. psittaci strain 6BC persistent infection cell model was induced using interferon (IFN)-gamma, alterations in the infectivity and morphology of the pathogen were analyzed, and the transcript profile of seven selected genes was analyzed. Following treatment with IFN-gamma, the infectivity of C. psittaci 6BC was decreased, the inclusion bodies appeared to be smaller, reticulate bodies were larger and the number of infectious elementary bodies was decreased compared with acute infection. In IFN-gamma-induced persistently infected cells, the relative mRNA expression levels of the genes CPSIT-0208, CPSIT-0310, CPSIT-0846, CPSIT-0844 and CPSIT-0594 were upregulated at 2-48 h post-infection (p.i.). The genes CPSIT-0959 and CPSIT-0057 were downregulated at 2-36 h p.i. The results of the present study advanced the understanding of C. psittaci persistent infection and demonstrated a number of previously unknown alterations in chlamydial gene expression, which may provide novel targets to further analyze this particular host-pathogen interaction.
通讯机构：
[Yimou Wu] I;Institution of Pathogenic Biology, Medical College, University of South China, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang 421001, China
摘要：
Treponema pallidum subsp. pallidum membrane proteins are considered as potent inducers in the initiation and development of inflammation. In the present study, the mechanism that leads to the production of interleukin 6 (IL-6), one of the key proinflammatory cytokines, by human monocytic THP-1 cells when these cells are treated with T. pallidum flagellin FlaA2 was investigated. Stimulation with flagellin FlaA2 can induce IL-6 expression in human monocytes and augment the phosphorylation of ERK, p38, and NF-κB, but has no effect on the phosphorylation of JNK. Likewise, FlaA2-induced IL-6 production was found to be attenuated by inhibitors for ERK, p38, and NF-κB, but not by JNK inhibitor. Immunofluorescence analysis showed that flagellin FlaA2 could stimulate the translocation of IκBα from the cytosol to the nucleus, and this phenomenon could be inhibited by the specific inhibitor BAY11-7082. FlaA2–induced IL-6 expression was also proved to be abrogated by transfection with dominant negative (DN) plasmid of MyD88. We further demonstrated that transfection with DN-TLR2 was sufficient to attenuate IL-6 expression and the phosphorylation of ERK, p38, and IκBα. These results suggest that flagellin FlaA2 induces IL-6 production via signaling pathways involving TLR2, MyD88, ERK, p38, and NF-κB in monocytes, which could contribute to the pathogenesis of T. pallidum.
作者机构：
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Diseases Prevention and Control;[刘海灿; 蒋毅; 连璐璐; 赵秀芹; 万康林] Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing, 102206;Fuzhou Pulmonary Hospital, Fuzhou, 350008;Pathogenic Biology Institute,University of South China, Hengyang, 421001;[刘海灿; 蒋毅; 连璐璐; 赵秀芹; 万康林] National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Diseases Prevention and Control
