作者机构：
[江艳; 伍永勤; 张平芳] School of Public Health, University of South China, Hengyang 421001, China;[王敏] The First Hospital of Changsha, Changsha 410011, China;[贺健梅; 陈曦; 魏秀青] Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China;[郭田生] Brain Hospital of Hunan Province, Changsha 410007, China;[Huang, G Q] Hengyang Center for Disease Control and Prevention, Hengyang 421001, China
通讯机构：
[Chen, X.] H;Hunan Provincial Center for Disease Control and Prevention, Changsha, China
摘要：
Increasing evidence indicates that lncRNAs play critical roles in various biological processes, but many have not been functionally characterized. Here, we report a novel radiation-inducible lncRNA, namely lnc-RI which is essential for cell survival and appropriate mitotic progression. Our data indicated that knockdown of lnc-RI resulted in spindle abnormalities and mitotic arrest simultaneously with sharply decreased mRNA and protein expression of PLK1, a key regulator of mitosis. Our data demonstrated that PLK1 is a key downstream mediator of lnc-RI in regulating mitosis, whereby lnc-RI competitively bound to the negative PLK1 regulating miRNA, miRNA-210-p3. Taken together, we have identified lnc-RI as a new regulator of mitosis which acts by releasing PLK1 mRNA activity via competition for binding to miRNA-210-3p.
通讯机构：
[Wang, Yong-Sheng] U;Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China.
关键词：
8-Hydroxy-2'-deoxyguanosine;Conformational switching;Fluorescence aptasensor;K(+)-stabilized G-quadruplex;N-methyl mesoporphyrin IX
摘要：
A sensitive, lable-free and low cost fluorescence aptasensor was developed for detecting 8-hydroxy-2'-deoxyguanosine (8-0HdG) by using 8-0HdG aptamer (Apt) as a recognition probe and N-methyl mesoporphyrin IX (NMM) as a reporter. The method is based on the conformational switching of a ICE-stabilized G-quadruplex to a 8-OHdG-stabilized one. NMM can selectively bind to K+-stabilized G-quadruplex instead of 8-OHdG-stabilized one. The addition of 8-0HdG in the solution of Apt - K+ ions leads to a sharp change in fluorescence intensity, which showed a good linear response toward 8-0HdG concentration ranging from 3.96 nM to 211 nM with a detection limit of 1.19 nM. The relative standard deviation and the recovery were 1.23-3.26% (n = 11) and 94.8-106.7%, respectively. The proposed aptasensor consists of only an aptamer probe and a specific dye NMM, avoiding the complex and expensive labeling procedure. Thus it is much cheaper and more applicable. (c) 2015 Elsevier B.V. All rights reserved.
摘要：
With rapid development of novel nanomaterials (NMs), the state of the art technologies with high efficiency and high-throughput characteristics had been applied for nanosafety evaluation. High-content screening (HCS), a cell-based multi-parametric image analysis technique, was adopted in the evaluation of eight different NMs in this study. A set of different endpoints including reactive oxygen species (ROS) production, Ca2+ transient, mitochondrial membrane potential (MMP) and cellular pH levels were checked in human bronchial epithelial (16HBE) cells after incubating with NMs for 24 hours. All NMs induced significant increase of intracellular ROS levels in 16HBE cells, although the decrease of cell viability was only found in Ag and ZnO NMs-treated cells. MMP level had a dose-response decrease in Ag, ZnO and CeO2 NMs-treated cells, while showed a significant increase in TiO2 NMs-treated cells. All tested NMs showed significant up-regulation of cellular lysosomal pH levels. However, none of NMs caused significant changes in cellular Ca2+ level at 24-hour time point. HCS allows for efficient and reliable screening of multiple responses of cells simultaneously within one screen test, which can avoid the problematic interpretation of investigations when carried on a single toxicological endpoint. Therefore, the present data provide insight and inspiration that HCS is an effective and powerful method for image-based assessments with a broad set of biological endpoints in toxicity evaluation of nanomaterials.
摘要：
A novel dual-channel strategy for the detection of metallothioneins (MTs) and Hg2+ has been developed based on a mercury-mediated aptamer beacon (MAB) using thymidine-mercury-thymidine complex as a quencher for the first time. In the presence of Hg2+, the T-rich oligonucleotide with a 6-carboxyfluorescein (TRO-FAM) can form an aptamer beacon via the formation of T-Hg2+-T base pairs, which results in a fluorescence quenching of the sensing system owing to the fluorescence resonance energy transfer (FRET) from the fluorophore of FAM to the terminated T-Hg2+-T base pair. The addition of MTs into this solution leads to the disruption of the T-Hg2+-T complex, resulting in an increase of the fluorescent signal of the system. In the optimizing condition, Delta F was directly proportional to the concentrations ranging from 5.63 nM to 0.275 mu M for MTs, and 14.2 nM to 0.30 mu M for Hg2+ with the detection limits of 1.69 nM and 4.28 nM, respectively. The proposed dual-channel method avoids the label steps of a quencher in common molecular beacon strategies, without tedious procedure or the requirement of sophisticated equipment, and is rapid, inexpensive and sensitive. (C) 2015 Elsevier B.V. All rights reserved.
摘要：
The intake of contaminated foods is an important exposure pathway for endocrine disrupting chemicals (EDCs). However, data on the occurrence of EDCs in foodstuffs are sporadic and the resultant risk of co-exposure is rarely concerned. In this study, 450 food samples representing 7 food categories (mainly raw and fresh food), collected from three geographic cities in China, were analyzed for eight EDCs using high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Besides estrone (E-1), other EDCs including diethylstilbestrol (DES), nonylphenol (NP), bisphenol A (BPA), octylphenol (OP), 17 beta-estradiol (E-2), 17 alpha-ethinylestradiol (EE2), and estriol (E-3) were ubiquitous in food. Dose-dependent relationships were found between NP and EE2 (r = 0.196, p < 0.05), BPA (r = 0.391, p < 0.05). Moreover, there existed a correspondence between EDCs congener and food category. Based on the obtained database of EDCs concentration combined with local food consumption, dietary EDCs exposure was estimated using the Monte Carlo Risk Assessment (MCRA) system. The 50th and 95th percentile exposure of any EDCs isomer were far below the tolerable daily intake (TDI) value identically. However, the sum of 17 beta-estradiol equivalents (Sigma EEQs) exposure in population was considerably larger than the value of exposure to E-2, which implied the underlying resultant risk of multiple EDCs in food should be concern. In conclusion, co-exposure via food consumption should be considered rather than individual EDCs during health risk evaluation. (C) 2015 Elsevier Ltd. All rights reserved.
作者：
Wang, J.;Zou, T.;Yang, H. X.;Gong, Y. Z.;Xie, X. J.;...
期刊：
Genetics and Molecular Research,2015年14(3):8819-8828 ISSN：1676-5680
通讯作者：
Liu, H. Y.
作者机构：
[Yang, H. X.; Wang, J.] Univ South China, Key Lab Atherosclerol Hunan Prov, Inst Cardiovasc Res, Hengyang, Hunan, Peoples R China.;[Yang, H. X.; Wang, J.] Univ South China, Sch Publ Hlth, Hengyang, Hunan, Peoples R China.;[Zou, T.] Univ South China, Affiliated Hosp 1, Dept Cardiovasc Med, Hengyang, Hunan, Peoples R China.;[Gong, Y. Z.; Xie, X. J.; Liao, D. F.] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha, Hunan, Peoples R China.;[Liu, H. Y.] Metammune LLC, Morrisville, NC 27560 USA.
通讯机构：
[Liu, H. Y.] M;Metammune LLC, Morrisville, NC 27560 USA.
期刊：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2015年15(12):9554-9564 ISSN：1533-4880
通讯作者：
Rang, Weiqing
作者机构：
[Rang, Weiqing; Shi, Xiaofei; Long, Dingxin; Zhao, Lin] Univ South China, Sch Publ Hlth, Hengyang 421001, Peoples R China.;[Shi, Xiaofei; Zhao, Lin; Chen, Chunying; Bai, Ru; Chen, Rui; Huo, Lingling] Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China.;[Pui, David Y. H.] Univ Minnesota, Coll Sci & Engn, Dept Mech Engn, Minneapolis, MN 55455 USA.;[Rang, Weiqing] Univ South China, Sch Publ Hlth, 28 Changshengxilu, Hengyang 421001, Peoples R China.
通讯机构：
[Rang, Weiqing] U;Univ South China, Sch Publ Hlth, 28 Changshengxilu, Hengyang 421001, Peoples R China.
关键词：
Printer;Nanoparticles Release;Attenuation;Size Distribution;Particle Number Concentration
摘要：
Indoor air quality has great impact on the human health. An increasing number of studies have shown that printers could release particulate matters and pose adverse effects on indoor air quality. In this study, a thorough investigation was designed to assess the aerosol printer particle total number concentration (TNC) and size distribution in normal office environment, one copy center, and a clean chamber. Particle analyzers, SMPS, OPS, and CPC3007 were used to monitor the total printing process. In normal office environment, 37 laser printers out of all surveyed 55 printers were classified as high particle emitters. Comparing to laser printers, 5 inkjet printers showed no particle emission. Particle emission level in a copy center increased slightly with TNC elevating to about 2 times of the aerosol background. Simulating test in a clean chamber indicated that printer-emitted particles were dominated by particles in nanoscale (diameter of particle, D-p < 100 nm). These particles in a sealed clean chamber attenuated so slowly that it still held at high level with the concentration of 1.5 x 10(4) particles/cm(3) after printing for 2.5 hours. Our present results demonstrate that printers indeed release particulates which keeping at a high concentration level in the indoor environment. Special care should be taken to this kind of widely applied machines and effective controls of particle emission at printing processes are necessary.
摘要：
【目的】初步探讨与单纯疱疹病毒糖蛋白D 竞争结合疱疹病毒侵入介体的淋巴毒素类似物(lymphotoxin-like inducible protein that competes with glycoprotein D for herpesvirus entry on T cells,LIGHT)在抗衣原体感染免疫及介导衣原体生殖道病理损伤过程的作用。【方法】用1 × 10~4 IFUs 的MoPn 经生殖道感染野生型(wild type,wt) 、LIGHT KO 小鼠,每组一半小鼠于感染后49d,再次感染相同剂量的MoPn。每隔3 - 4 d取生殖道分泌物,测定其中衣原体包涵体的数量。初次感染后80d,处死小鼠,眼眶取血,分离血清,用间接免疫荧光法测定其中抗体类型及效价; 同时分离生殖道,肉眼观察其输卵管、子宫角水肿程度,然后甲醛固定、切片,H&E 染色后,显微镜下观察各组织炎性浸润程度和管腔水肿程度。分离小鼠脾细胞,体外用衣原体EB 刺激,测定上清中IL-4、IL-5、IL-17和IFN-γ 等细胞因子水平。【结果】LIGHT KO 小鼠阴道带菌时间与wt 组相当,大部分小鼠均在原发感染后28d 左右完全清除感染,且均产生对再次感染的免疫力。LIGHT KO 和wt 小鼠子宫角和输卵管均出现一定程度的病变,但差异无统计学意义。两组小鼠在原发和继发感染MoPn 后,均产生高效价的特异性抗MoPn IgG 抗体,总抗体及各IgG 抗体亚类效价差异均无统计学意义(P > 0. 05),且IgG2a /IgG1比值均大于1。和wt 小鼠一样,LIGHT KO 小鼠脾淋巴细胞经衣原体再次刺激后均可产生较高水平的IFN-γ 和IL-17,且未能检测到IL-4和IL-5。【结论】小鼠抗MoPn 生殖道感染及MoPn 引起的生殖道病理损伤不依赖于LIGHT 信号通路。
