摘要：
Ursolic acid (UA) is a pentacyclic triterpenoid with diverse biological activities, especially in the fields of cardiovascular and diabetes treatment. However, its application is hindered by low bioavailability and poor solubility in water. Consequently, reseacher have focused on designing UA derivatives to address these issues. This paper provides an overview of the development of UA derivatives and their recent advancements as regents for combating cardiovascular diseases and diabetes. Abstract Ursolic acid (UA) is a pentacyclic triterpenoid, which exhibits many biological activities, particularly in anti‐cardiovascular and anti‐diabetes. The further application of UA is greatly limited due to its low bioavailability and poor water solubility. Up to date, various UA derivatives have been designed to overcome these shortcomings. In this paper, the authors reviewed the development of UA derivatives as the anti‐diabetes anti‐cardiovascular reagents.
作者：
Wang Wen-jing;Wang Yi-fu;Jin Ya-jie;Song Wu-qiang;Lin Jia-meng;...
期刊：
农业科学学报(英文),2023年22(1):320-324 ISSN：2095-3119
作者机构：
[Wang Wen-jing; Tu Jian; Wang Yi-fu] Univ South China, Inst Pharm & Pharmacol, Hunan Prov Key Lab Tumor Microenvironm Respons Dr, Hengyang 421001, Peoples R China.;[Wang Wen-jing; Tong Xin-ru; Jin Ya-jie; Lin Jia-meng; Zhang Yan; Wang Yi-fu; Li Tao; Song Wu-qiang] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China.;[Tu Jian] Guilin Med Univ, Guilin 541199, Peoples R China.;[Li Rui-chao] Yangzhou Univ, Coll Vet Med, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China.
摘要：
An extensively drug-resistant (XDR) Escherichia coli strain 258E was isolated from an anal swab sample of a chicken farm of Anhui Province in China. Genomic analyses indicated that the strain 258E harbors an incompatibility group N (IncN) plasmid pEC258-3, which co-produces bla_(CTX-M-3), bla_(KPC-2), bla_(TEM-1B), qnrS1, aac(6')-Ib-cr, dfrA14, arr-3, and aac(6')-Ib3. Multiple genome arrangement analyses indicated that pEC258-3 is highly homologous with pCRKP-1-KPC discovered in Klebsiella pneumoniae from a patient. Furthermore, conjugation experiments proved that plasmid pEC258-3 can be transferred horizontally and may pose a significant potential threat in animals, community and hospital settings.
摘要：
Purpose: To investigate the effect of emodin on osteosarcoma cell proliferation and apoptosis.Methods: The osteosarcoma cell proliferation ability was determined by CCK-8, apoptosis level and cell cycle were determined via flow cytometry. Honechst staining was used to determine the ratio of nuclear pyknosis, Western blotting was used to determine the expression of AKT, p-AKT and caspase-3 precursor protein in each group of osteosarcoma cells.Results: Emodin significantly inhibited the MG-63 cell proliferation and promoted the apoptosis of osteosarcoma cells in a dose-dependent manner (p < 0.05). It further blocked osteosarcoma cells in G1/G0 phase and decreased the expression of AKT, p-AKT, and caspase-3 (p < 0.05).Conclusion: Emodin inhibits osteosarcoma cell growth by inhibiting ATM protein cleavage. There is a need to carry out further investigation of the effect of emodin on osteosarcoma cells using animal models in order to ascertain its potential in the management of osteosarcoma.
摘要：
Long non-coding RNA (lncRNA) is a kind of biomolecule that can regulate important life activities such as cell proliferation, apoptosis, differentiation, aging, and body development. It has been found that lncRNAs are closely related to various diseases. In cardiovascular diseases, lncRNAs affect the expression level of related genes in atherosclerotic plaques, which are closely related to endothelial dysfunction, smooth muscle cell proliferation, macrophage dysfunction, abnormal lipid metabolism, and cellular autophagy, thus participating in regulating the occurrence and development of AS. In view of this, investigating the role of lncRNAs in regulating cardiac gene networks on cardiovascular system diseases has attracted much clinical attention and may be a novel target for AS therapy. This paper focuses on lncRNAs related to AS, explores the relationship between lncRNAs and AS, suggests the role of lncRNAs in the prevention and treatment of AS, and expects the application of more lncRNAs as the marker in the clinical diagnosis and treatment of AS.
摘要：
The endoplasmic reticulum (ER) is one of the most important organelles in cells, involved in protein synthesis, folding, and modification, as well as Ca2+ storage and release. ER homeostasis imbalances may contribute to various diseases, including neurodegenerative diseases, cardiovascular diseases, metabolic diseases, and cancer. Current research efforts primarily focus on ER-related disease mechanisms and drug candidate screening. Because disease processes involve multiple molecular events and signaling, an innovative tool is necessary to precisely detect key signaling molecules in the ER to accelerate the discovery of targeted therapeutics. In recent years, because of their real-time visualization, precise targeting, high sensitivity and specificity, low cytotoxicity, and synthetic tunability, fluorescent probes have been widely used to investigate biological processes and disease events. This review first introduces ER and its related biological processes, and the design rules of ER-targeted probes. Subsequently, based on the latest literature, it systematically summarizes the research progress of fluorescent probes for imaging physiological or pathological events in the ER. Lastly, it discusses the challenges of ER-targeted probes as well as the future development of probe engineering. We anticipate that this review will not only encourage the development of fluorescence probe engineering but will also enhance interdisciplinary research between chemistry, biology, pharmacology, and medicine, promoting for its widespread application.
通讯机构：
[Linxi Chen; Lanfang Li] I;Institute of Pharmacy and Pharmacology, Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, 421001, Hunan, China
摘要：
Glycometabolism is well known for its roles as the main source of energy, which mainly includes three metabolic pathways: oxidative phosphorylation, glycolysis and pentose phosphate pathway. The orderly progress of glycometabolism is the basis for the maintenance of cardiovascular function. However, upon exposure to harmful stimuli, the intracellular glycometabolism changes or tends to shift toward another glycometabolism pathway more suitable for its own development and adaptation. This shift away from the normal glycometabolism is also known as glycometabolism reprogramming, which is commonly related to the occurrence and aggravation of cardiovascular diseases. In this review, we elucidate the physiological role of glycometabolism in the cardiovascular system and summarize the mechanisms by which glycometabolism drives cardiovascular diseases, including diabetes, cardiac hypertrophy, heart failure, atherosclerosis, and pulmonary hypertension. Collectively, directing GMR back to normal glycometabolism might provide a therapeutic strategy for the prevention and treatment of related cardiovascular diseases.
期刊：
Bioinorganic Chemistry and Applications,2023年2023 ISSN：1565-3633
通讯作者：
Li, R.;Feng, W.
作者机构：
[Song, Jingfang; Feng, Wei] Univ South China, Affiliated Hosp 2, Inst Pathogen Biol, Hengyang Med Sch,Dept Pediat, Hengyang 421001, Hunan, Peoples R China.;[Song, Jingfang; Feng, Wei; Li, Ranhui] Hunan Prov Key Lab Special Pathogens Prevent & Con, Hengyang 421001, Hunan, Peoples R China.;[Wang, Weiguo; Yang, Lin; He, Jian] Univ South China, Inst Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Bai, Qinqin] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Dept Publ Hlth Lab Sci, Hengyang 421001, Hunan, Peoples R China.;[Li, Ranhui] Univ South China, Inst Pathogen Biol, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Feng, W.] T;[Li, R.] I;Institute of Pathogenic Biology Hengyang Medical School University of South China, China;The Second Affiliated Hospital Department of Pediatrics Institute of Pathogenic Biology Hengyang Medical School University of South China, China
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
作者机构：
[Song, Zhiyin; Yu, Jianglong; He, He; Zhao, Huabin; Guo, Hanze; He, H; Gong, Longlong; Hao, Tianshu; Wu, Zhida; Wang, Bingjun; Jiang, Jie] Wuhan Univ, Renmin Hosp, Taikang Ctr life & Med Sci, Coll Life Sci,Frontier Sci Ctr Immunol & Metab,Dep, Wuhan 430072, Hubei, Peoples R China.;[Lu, Bin] Univ South China, Sch Basic Med Sci, Hengyang Med Sch, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.;[Engelender, Simone] Technion Israel Inst Technol, Rappaport Fac Med, Dept Biochem, Haifa, Israel.
通讯机构：
[He, H; Song, ZY ] W;Wuhan Univ, Renmin Hosp, Taikang Ctr life & Med Sci, Coll Life Sci,Frontier Sci Ctr Immunol & Metab,Dep, Wuhan 430072, Hubei, Peoples R China.
摘要：
Mitochondria are the key organelles for sensing oxygen, which is consumed by oxidative phosphorylation to generate ATP. Lysosomes contain hydrolytic enzymes that degrade misfolded proteins and damaged organelles to maintain cellular homeostasis. Mitochondria physically and functionally interact with lysosomes to regulate cellular metabolism. However, the mode and biological functions of mitochondria-lysosome communication remain largely unknown. Here, we show that hypoxia remodels normal tubular mitochondria into megamitochondria by inducing broad inter-mitochondria contacts and subsequent fusion. Importantly, under hypoxia, mitochondria-lysosome contacts are promoted, and certain lysosomes are engulfed by megamitochondria, in a process we term megamitochondria engulfing lysosome (MMEL). Both megamitochondria and mature lysosomes are required for MMEL. Moreover, the STX17-SNAP29-VAMP7 complex contributes to mitochondria-lysosome contacts and MMEL under hypoxia. Intriguingly, MMEL mediates a mode of mitochondrial degradation, which we termed mitochondrial self-digestion (MSD). Moreover, MSD increases mitochondrial ROS production. Our results reveal a mode of crosstalk between mitochondria and lysosomes and uncover an additional pathway for mitochondrial degradation. Several organelle membranes make contact in the cell, with many contacts being spatially segregated sites dedicated to specific functions. Here, Hao et al. show that hypoxia increases mitochondria-lysosome contacts, leading to engulfment and degradation of the mitochondria.
摘要：
BACKGROUND AND PURPOSE: Research has revealed the involvement of mitochondrial autophagy and iron death in the pathogenesis of myocardial fibrosis. The objective of this study is to investigate whether the mitochondrial-targeted H(2)S donor AP39 inhibits mitochondrial autophagy and antagonizes myocardial cell iron death through the PINK1/Parkin pathway, thereby improving myocardial fibrosis in rats with myocardial infarction. EXPERIMENTAL APPROACH: A rat model of myocardial infarction was created by intraperitoneal injection of a high dose of isoproterenol, and H9c2 myocardial cells were subjected to hypoxic injury induced by CoCl(2). Western blot, RT-PCR, transmission electron microscopy, immunohistochemistry, as well as echocardiography, and studies on isolated hearts were employed. KEY RESULTS: In the hearts of rats with myocardial infarction, there was a significant accumulation of interstitial collagen fibers, accompanied by downregulation of CSE protein expression, activation of the PINK1/Parkin signaling pathway, and activation of mitochondrial autophagy. Intervention with AP39 resulted in a significant improvement of the aforementioned changes, which could be reversed by the addition of PAG. Similar results were observed in vitro experiments. Furthermore, the addition of CCCP reversed the antagonistic effect of AP39 on myocardial cell iron death, while the addition of RSL3 reversed the inhibitory effect of AP39 on collagen production in myocardial cells. CONCLUSION AND IMPLICATIONS: The mitochondrial-targeted H(2)S donor AP39 can inhibit mitochondrial autophagy through the PINK1/Parkin pathway, antagonize myocardial cell iron death, and improve myocardial fibrosis in rats with myocardial infarction.
作者机构：
[Tang, Caihong; Jiang, Zhong-Xing; Yao, Xu; Zheng, Xing] Univ South China, Inst Pharm & Pharmacol, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Jianxin; Tang, Feng; Shi, Wei; Zeng, Yue; Tang, Caihong; Huang, Wei] Chinese Acad Sci, Shanghai Inst Mat Med, CAS Ctr Excellence Mol Cell Sci, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China.;[Shi, Wei; Huang, Wei] Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China.;[Huang, Wei] Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China.;[Huang, Wei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
通讯机构：
[Xu Yao; Zhong-Xing Jiang] I;[Wei Shi; Wei Huang] C;CAS Key Laboratory of Receptor Research, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, No. 555 Zuchongzhi Road, Pudong, Shanghai 201203, China<&wdkj&>School of Pharmaceutical Science and Technology, Hangzhou Institute of Advanced Study, Hangzhou 310024, China<&wdkj&>School of Chinese Materia Medica, Nanjing University of Chinese Medicine, No. 138 Xianlin Road, Nanjing 210023, China<&wdkj&>University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China<&wdkj&>Shanghai GlycanLink Biotech. Co. Ltd., Minhang, Shanghai 201203, China<&wdkj&>Institute of Pharmacy and Pharmacology, Hengyang Medicinal School, University of South China, Hengyang, Hunan 421001, China<&wdkj&>CAS Key Laboratory of Receptor Research, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, No. 555 Zuchongzhi Road, Pudong, Shanghai 201203, China<&wdkj&>School of Pharmaceutical Science and Technology, Hangzhou Institute of Advanced Study, Hangzhou 310024, China
摘要：
The drug-to-antibody ratio (DAR) value and dual-drug combination greatly influence the therapeutic index of antibody–drug conjugates (ADCs). The reported approaches usually require multifunctional branched linkers, a combination of complicated technologies, or protein–protein ligation, which may incorporate multihydrophobic fragments or result in low coupling efficiency. Herein, we developed a facile and efficient one-pot method to assemble dual-site-specific ADCs with defined DARs at both the N-glycosylation site and K248 site, either with the same payloads or with two types of payloads. The constructed dual-site ADCs showed acceptable homogeneity, excellent buffer stability, and enhanced in vitro and in vivo efficiency.
期刊：
International Journal of Molecular Sciences,2023年24(8):7013- ISSN：1422-0067
通讯作者：
Yimou Wu<&wdkj&>Shuzhi Wang
作者机构：
[Lu, Chunxue; Wu, Yimou; Lei, Aihua; Wang, Jiewen; Zheng, Kang; Jin, Yingqi; Wang, Chuan; Wang, Shuzhi] Univ South China, Inst Pathogen Biol, Hengyang Med Coll, Sch Basic Med, Hengyang 421001, Peoples R China.;[Lu, Chunxue; Wu, Yimou; Lei, Aihua; Wang, Jiewen; Zheng, Kang; Jin, Yingqi; Wang, Chuan; Wang, Shuzhi] Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Con, Hengyang 421001, Peoples R China.;[Zheng, Kang] Hengyang Cent Hosp, Dept Clin Lab, Hengyang 421001, Peoples R China.;[Wang, Shuzhi] Univ South China, Hengyang Med Coll, Sch Pharmaceut Sci, Dept Pharmacol, Hengyang 421001, Peoples R China.
通讯机构：
[Yimou Wu; Shuzhi Wang] A;Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang 421001, China<&wdkj&>Institute of Pathogenic Biology, School of Basic Medicine, Hengyang Medical College, University of South China, Hengyang 421001, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, University of South China, Hengyang 421001, China<&wdkj&>Institute of Pathogenic Biology, School of Basic Medicine, Hengyang Medical College, University of South China, Hengyang 421001, China<&wdkj&>Department of Pharmacology, School of Pharmaceutical Science, Hengyang Medical College, University of South China, Hengyang 421001, China
摘要：
Chlamydia psittaci (C. psittaci), a zoonotic pathogen, poses a potential threat to public health security and the development of animal husbandry. Vaccine-based preventative measures for infectious diseases have a promising landscape. DNA vaccines, with many advantages, have become one of the dominant candidate strategies in preventing and controlling the chlamydial infection. Our previous study showed that CPSIT_p7 protein is an effective candidate for a vaccine against C. psittaci. Thus, this study evaluated the protective immunity of pcDNA3.1(+)/CPSIT_p7 against C. psittaci infection in BALB/c mice. We found that pcDNA3.1(+)/CPSIT_p7 can induce strong humoral and cellular immune responses. The IFN-gamma and IL-6 levels in the infected lungs of mice immunized with pcDNA3.1(+)/CPSIT_p7 reduced substantially. In addition, the pcDNA3.1(+)/CPSIT_p7 vaccine diminished pulmonary pathological lesions and reduced the C. psittaci load in the lungs of infected mice. It is worth noting that pcDNA3.1(+)/CPSIT_p7 suppressed C. psittaci dissemination in BALB/c mice. In a word, these results demonstrate that the pcDNA3.1(+)/CPSIT_p7 DNA vaccine has good immunogenicity and immunity protection effectiveness against C. psittaci infection in BALB/c mice, especially pulmonary infection, and provides essential practical experience and insights for the development of a DNA vaccine against chlamydial infection.
通讯机构：
[Li-Chen Gao] S;School of Pharmacy, Department of Pharmacy, Phase I Clinical Trial Centre, the Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, Changsha, China
摘要：
Ferrous ion (Fe2+) is a crucial metal ion in the body and participates in the diseases related to oxidation and reduction. Golgi apparatus is the main subcellular organelle of Fe2+ transport in cells, and the stability of its structure is related to the Fe2+ at an appropriate concentration. In this work, a turn-on type Golgi-targeting fluorescent chemosensor Gol-Cou-Fe2+ was rationally designed for sensitive and selective detection of Fe2+. Gol-Cou-Fe2+ showed excellent capacity of detecting exogenous and endogenous Fe2+ in HUVEC and HepG2 cells. It was used to capture the up-regulated Fe2+ level during the hypoxia. Moreover, the fluorescence of sensor was enhanced over time under Golgi stress combining with the reduce of Golgi matrix protein GM130. However, elimination of Fe2+ or addition of nitric oxide (NO) would restore the fluorescence intensity of Gol-Cou-Fe2+ and the expression of GM130 in HUVEC. Thus, development of chemosensor Gol-Cou-Fe2+ provides a new window for tracking Golgi Fe2+ and elucidating Golgi stress-related diseases.
作者机构：
[Chen, Yuping; Wu, Meichun; Chen, YP] Univ South China, Hengyang Med Sch, Hengyang 410001, Hunan, Peoples R China.;[Wu, Meichun] Univ South China, Sch Nursing, Hengyang 410001, Hunan, Peoples R China.;[Chen, Yuping; Xun, Min; Chen, YP] Univ South China, Inst Pharm & Pharmacol, Sch Pharmaceut Sci, Hengyang 410001, Hunan, Peoples R China.
通讯机构：
[Chen, YP ] U;Univ South China, Hengyang Med Sch, Hengyang 410001, Hunan, Peoples R China.;Univ South China, Inst Pharm & Pharmacol, Sch Pharmaceut Sci, Hengyang 410001, Hunan, Peoples R China.
关键词：
biodegradable metal stents;vascular smooth muscle cell;stent implantation;vascular microenvironment;atherosclerosis;restenosis
摘要：
Iron-, magnesium-, or zinc-based metal vessel stents support vessel expansion at the period early after implantation and degrade away after vascular reconstruction, eliminating the side effects due to the long stay of stent implants in the body and the risks of restenosis and neoatherosclerosis. However, emerging evidence has indicated that their degradation alters the vascular microenvironment and induces adaptive responses of surrounding vessel cells, especially vascular smooth muscle cells (VSMCs). VSMCs are highly flexible cells that actively alter their phenotype in response to the stenting, similarly to what they do during all stages of atherosclerosis pathology, which significantly influences stent performance. This Review discusses how biodegradable metal stents modify vascular conditions and how VSMCs respond to various chemical, biological, and physical signals attributable to stent implantation. The focus is placed on the phenotypic adaptation of VSMCs and the clinical complications, which highlight the importance of VSMC transformation in future stent design.
摘要：
The dysregulation of nuclear factor erythroid 2-related factor 2 (Nrf2) and/or solute carrier family 7 member 11 (SLC7A11) is believed to contribute to ferroptosis in the hearts suffered ischemia/reperfusion (I/R), but the mechanisms behind the dysregulation of them are not fully elucidated. Mucosa associated lymphoid tissue lymphoma translocation gene 1 (MALT1) can function as a paracaspase to cleave specified substrates and it is predicted to interact with Nrf2. This study aims to explore whether targeting MALT1 can reduce I/R-induced ferroptosis via enhancing the Nrf2/SLC7A11 pathway. The SD rat hearts were subjected to 1h-ischemia plus 3h-reperfusion to establish the I/R injury model, which showed myocardial injuries (increase in infarct size and creatine kinase release) and up-regulation of MALT1 while downregulation of Nrf2 and SLC7A11 concomitant with the increased ferroptosis, reflecting by an increase in glutathione peroxidase 4 (GPX4) level while decreases in the levels of acyl-CoA synthetase long chain family member 4 (ACSL4), total iron, Fe(2+) and lipid peroxidation (LPO); these phenomena were reversed in the presence of MI-2, a specific inhibitor of MALT1. Consistently, similar results were achieved in the cultured cardiomyocytes subjected to 8h-hypoxia plus 12h-reoxygenation. Furthermore, micafungin, an antifungal drug, could also exert beneficial effect on mitigating myocardial I/R injury via inhibition of MALT1. Based on these observations, we conclud that inhibition of MALT1 can reduce I/R-induced myocardial ferroptosis through enhancing the Nrf2/SLC7A11 pathway; and MALT1 may be used as a potential target to seek novel or existing drugs (such as micafungin) for treating myocardial infarction.
通讯机构：
[Li-Chen Gao] S;School of Pharmacy, Department of Pharmacy, Phase Ⅰ Clinical Trial Centre, Changsha Central Hospital Affiliated to University of South China, University of South China, Hengyang, Hunan, China<&wdkj&>Corresponding Author:<&wdkj&>Li-Chen Gao, School of Pharmacy, Department of Pharmacy, Phase Ⅰ Clinical Trial Centre, Changsha Central Hospital Affiliated to University of South China, University of South China, Hengyang, Hunan, 421001, China
摘要：
Cis-regulatory elements are important molecular switches in controlling gene expression and are regarded as determinant hubs in the transcriptional regulatory network. Collection and processing of large-scale cis-regulatory data are urgent to decipher the potential mechanisms of cardiovascular diseases from a cis-regulatory element aspect. Here, we developed a novel web server, Cis-Cardio, which aims to document a large number of available cardiovascular-related cis-regulatory data and to provide analysis for unveiling the comprehensive mechanisms at a cis-regulation level. The current version of Cis-Cardio catalogs a total of 45,382,361 genomic regions from 1,013 human and mouse epigenetic datasets, including ATAC-seq, DNase-seq, Histone ChIP-seq, TF/TcoF ChIP-seq, RNA polymerase ChIP-seq, and Cohesin ChIP-seq. Importantly, Cis-Cardio provides six analysis tools, including region overlap analysis,element upstream/downstream analysis, transcription regulator enrichment analysis, variant interpretation, andprotein-protein interaction-based co-regulatory analysis. Additionally, Cis-Cardio provides detailed and abundant (epi-) genetic annotations in cis-regulatory regions, such as super-enhancers, enhancers, transcription factor binding sites (TFBSs), methylation sites, common SNPs, risk SNPs, expression quantitative trait loci (eQTLs), motifs,DNase I hypersensitive sites (DHSs), and 3D chromatin interactions. In summary, Cis-Cardio is a valuable resourcefor elucidating and analyzing regulatory cues of cardiovascular-specific cis-regulatory elements. The platform is freely available at http://www.licpathway.net/Cis-Cardio/index.html.
摘要：
Nasopharyngeal carcinoma (NPC) is a highly recurrent and metastatic malignant tumor affecting a large number of individuals in southern China. Traditional Chinese herbal medicine has been found to be a rich source of natural compounds with mild therapeutic effects and minimal side effects, making them increasingly popular for treating various diseases. Trifolirhizin, a natural flavonoid derived from legu-minous plants, has gained significant attention for its therapeutic potential. In this study, we confirmed that trifolirhizin could effectively inhibit the proliferation, migration and invasion of nasopharyngeal carcinoma 6-10B and HK1 cells. Furthermore, our findings demonstrated that trifolirhizin achieves this by suppressing the PI3K/Akt signaling pathway. The findings of the present study provides a valuable perspective on the potential therapeutic applications of trifolirhizin for the treatment of nasopharyngeal carcinoma.(c) 2023 Elsevier Inc. All rights reserved.
