期刊:
Journal of Translational Medicine,2022年20(1):1-11 ISSN:1479-5876
通讯作者:
Jie Li<&wdkj&>Weiqiang Tang
作者机构:
[Li, Jie; Liao, Yajie] Univ South China, Inst Pharm & Pharmacol, Hengyang Med Sch, Peoples Hosp Chenzhou 1, Chenzhou 423000, Hunan, Peoples R China.;[Wu, Xudong] Third Hosp Changsha, Dept Thorac Surg, Changsha 410035, Peoples R China.;[Wu, Mengyu] Jianghan Univ, Sch Med, Wuhan 430056, Peoples R China.;[Fang, Yuan] Kunming Med Univ, Affiliated Hosp 1, Organ Transplantat Ctr, Kunming 650032, Yunnan, Peoples R China.;[Tang, Weiqiang] Univ South China, Hengyang Med Sch, Inst Clin Med, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Jie Li; Weiqiang Tang] I;Institute of Pharmacy and Pharmacology, The First People’s Hospital of Chenzhou, Hengyang Medical School, University of South China, Chenzhou, People’s Republic of China<&wdkj&>Institute of Clinical Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, People’s Republic of China
摘要:
Lung cancer is the second cancer and the leading cause of tumor-related mortality worldwide. Angiogenesis is a crucial hallmark of cancer development and a promising target in lung cancer. However, the anti-angiogenic drugs currently used in the clinic do not achieve long-term efficacy and are accompanied by severe adverse reactions. Therefore, the development of novel anti-angiogenic therapeutic approaches for lung cancer is urgently needed. Non-coding RNAs (ncRNAs) participate in multiple biological processes in cancers, including tumor angiogenesis. Many studies have demonstrated that ncRNAs play crucial roles in tumor angiogenesis. This review discusses the regulatory functions of different ncRNAs in lung cancer angiogenesis, focusing on the downstream targets and signaling pathways regulated by these ncRNAs. Additionally, given the recent trend towards utilizing ncRNAs as cancer therapeutics, we also discuss the tremendous potential applications of ncRNAs as biomarkers or novel anti-angiogenic tools in lung cancer.
通讯机构:
[Li-Chen Gao] S;School of Pharmacy, Department of Pharmacy, Phase I Clinical Trial Centre, Changsha Central Hospital Affiliated to University of South China, University of South China, Hengyang, China
摘要:
BACKGROUND: Cloperastine is a pivotal antibechic widely prescribed to treat cough caused by respiratory diseases. The present trial evaluated the pharmacokinetics (PK), bioequivalence (BE) and safety effects of the generic test (T) tablet of cloperastine after single-dose administration of cloperastine, compared with the original reference (R) tablet of cloperastine. OBJECTIVE: The purpose of this trial was to compare the PK, BE and safety of a test 10 mg versus the reference 10 mg formulation of cloperastine under fasting and postprandial conditions in healthy Chinese volunteers. METHODS: A single-centre, randomised, open, double-cycle, self-crossover, single oral administration Phase I trial was performed in healthy Chinese volunteers. A total of 60 subjects were enrolled in either the fasting (28 subjects) or the postprandial condition (32 subjects). Subjects randomly received a single dose of the T or R preparation (10 mg dose). Plasma concentrations of cloperastine were analysed by a validated LC-MS/MS method. The primary endpoints of the PK parameters were the area under the plasma concentration-time curve from zero to 72 h (AUC(0-72h)), under the plasma concentration-time curve from zero to infinity (AUC(0-∞)) and the maximal plasma concentration (C(max)). The equivalence standard range (80.0-125.0%) was used to evaluate the BE of the two preparations. The safety parameter as secondary endpoint was mainly evaluated by the occurrence of adverse events (AEs). RESULTS: A total of 25 and 30 subjects in the fasting and postprandial conditions completed this clinical trial, respectively. The geometric mean ratio (GMR) of the T/R for the C(max), AUC(0-72h) and AUC(0-∞) were 102.1%, 103.8% and 104.0% in the fasting condition, respectively. In the postprandial condition, the GMR of the T/R for the C(max), AUC(0-72h) and AUC(0-∞) were 94.2%, 98.8% and 99.0%, respectively. All the values fell within the range (80.0-125.0%). The C(max) and AUC(0-72h) values of the T and R preparations in fasting and postprandial conditions were not statistically significant (P > 0.05). Furthermore, no serious adverse events (SAEs) occurred during the whole trial. CONCLUSIONS: The T and R preparations were bioequivalent under both conditions. Food has no significant effect on the absorption of cloperastine. Moreover, T and R preparations were well tolerated. The trial registration number (TRN) and date of registrations were CTR20212515, 13 October 2021.
作者机构:
[Gao, Jiayin; Gao, Anbo; Chen, Linxi] Univ South China, Inst Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Learning Key Lab Pharmacoprote, Hengyang 421001, Peoples R China.;[Zhou, Hong] Univ South China, Affiliated Hosp 1, Dept Radiol, Hengyang 421001, Peoples R China.
通讯机构:
[Hong Zhou] D;[Linxi Chen] I;Department of Radiology of the First Affiliated Hospital of University of South China, Hengyang, China<&wdkj&>Institute of Pharmacy and Pharmacology, Learning Key Laboratory for Pharmacoproteomics, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China
作者机构:
[Cao, Zhaohui; Hu, Xiaobo; Tang, Cifei; Zeng, Min; Huang, Di] Univ South China, Hengyang Med Sch, Sch Basic Med, Dept Biochem & Mol Biol, Hengyang 421001, Peoples R China.;[Cao, Zhaohui; Hu, Xiaobo; Tang, Cifei; Zeng, Min; Huang, Di] Univ South China, Hengyang Med Sch, Key Lab Ecol Environm & Crit Human Dis Prevent Hu, Dept Educ, Hengyang 421001, Peoples R China.
通讯机构:
[Xiaobo Hu] D;Department of Biochemistry and Molecular Biology, School of Basic Medicine, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>The Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province Department of Education, Hengyang Medical School, University of South China, Hengyang, China
关键词:
OXIDATIVE;predominant;NOX2
摘要:
Neutrophils are predominant leukocytes in the circulation, which are essential for killing invading pathogens via the activation of effector responses and the production of reactive oxygen species (ROS), also named as "oxidative burst." When infected, activated neutrophils fight bacteria, fungi, and viruses through oxidative burst, phagocytosis, degranulation, and the production of neutrophil extracellular traps (NETs) in a neutrophil death process named as "NETosis" (Mutua and Gershwin, 2021). NETs, consisting of DNA fibers decorated with modified histones and numerous antimicrobial proteins from cytoplasmic granules and the nucleus, can either be beneficial or detrimental (Mutua and Gershwin, 2021). Several pathways can lead to this death process. In response to various stimuli, NETosis traps and clears pathogens, facilitating phagocytosis by other neutrophils and phagocytes. However, excessive NETosis often results in disease due to increasing the pro-inflammatory response and perpetuating the inflammatory condition (Hellebrekers et al., 2018; Hidalgo et al., 2019; Klopf et al., 2021). Accordingly, inhibiting aberrant NETosis may alleviate the severity of various autoimmune and inflammatory diseases.
摘要:
Inositol 1, 4, 5-trisphosphate (IP3) signaling-mediated calcium release drives the contraction of vascular smooth muscles and hence regulates blood vessel volume and blood pressure. Melatonin supplementation has been suggested to be beneficial for hypertension. To determine whether the blood pressure-lowering effect of melatonin was accounted for by IP3 signaling, we evaluated the vasoconstriction response and IP3 signaling in isolated mouse thoracic aortic rings during melatonin incubation. C57BL/6 mice were given intraperitoneal injections daily with melatonin, and the systolic blood pressure and contractility of aortic rings from melatonin-treated mice were decreased, and the contraction suppression effect of melatonin was attributed to the impaired expression of contractile proteins in vascular smooth muscle cells rather than IP3 signaling. Our results further showed that melatonin increased the expression of γ-secretase, which could cleave and release the notch intracellular domain, and the notch intracellular domain prevented the transcription of contractile genes by interfering with the interaction between serum response factor and myocardin, the master regulator of contractile protein. In this article, we report a novel mechanism by which melatonin regulates smooth muscle contractility that does not depend on IP3 signaling.
作者机构:
[Duan, Dan-Qing; Peng, Jun; Zhou, Yuan-Jing; Lu, Li-Qun; Zhang, Xiao-Jie] Cent South Univ, Xiangya Sch Pharmaceut Sci, Dept Pharmacol, Changsha 410078, Peoples R China.;[Zhou, Yuan-Jing] Guangdong Med Univ, Zhanjiang Cent Hosp, Dept Pharm, Zhanjiang 524045, Peoples R China.;[Tang, Li-Jing] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Dept Pharm, Hengyang 421001, Hunan, Peoples R China.;[Luo, Xiu-Ju] Cent South Univ, Xiangya Hosp 3, Dept Lab Med, Changsha 410013, Peoples R China.;[Peng, Jun] Cent South Univ, Sch Pharmaceut Sci, Hunan Prov Key Lab Cardiovasc Res, Changsha 410078, Peoples R China.
通讯机构:
[Jun Peng] D;Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China<&wdkj&>Hunan Provincial Key Laboratory of Cardiovascular Research, School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China
摘要:
Ferroptosis is an iron-dependent cell death and contributes to doxorubicin-induced cardiotoxicity, but the mechanisms behind intracellular iron overload in cardiomyocyte after administration of doxorubicin remain largely unknown. Ferritinophagy is a selective type of autophagy and could be a novel source for intracellular free iron. Spermatogenesis-associated protein 2 (SPATA2), a member of the TNF signaling pathway, can recruit cylindromatosis (CYLD, a deubiquitinating enzyme) to regulate cell death. This study aims to explore whether ferritinophagy is the source for intracellular iron overload in cardiomyocyte upon doxorubicin treatment and whether the SPATA2/CYLD pathway is involved in regulation of nuclear receptor coactivator 4 (NCOA4) level, the selective cargo receptor for ferritinophagy. The C57BL/6J mice were subjected to a single injection of doxorubicin, which showed the compromised cardiac functions, accompanied by the upregulation of SPATA2 and CYLD and the enhanced interaction between them, the increases in ferritinophagy (reflecting by increases in NCOA4 and ratio of LC3Ⅱ/LC3Ⅰ while decreases in NCOA4 ubiquitination and ferritin) and ferroptosis (reflecting by intracellular iron overload and increase of acyl-CoA synthetase long chain family member 4). Consistently, similar results were achieved in the cultured cardiomyocytes after incubation with doxorubicin. Knocked down of SPATA2 notably reduced doxorubicin-induced cardiomyocyte injury concomitant with the attenuated ferritinophagy and the decreased ferroptosis. Based on these observations, we conclude that a novel pathway of SPATA2/CYLD has been identified, which contributes to doxorubicin-induced cardiomyocyte ferroptosis via enhancing ferritinophagy through a mechanism involving the deubiquitination of NCOA4.
作者机构:
[Wu, Yueyue; Chen, Liesong; Zhu, Cuiming; You, Xiaoxing; Zeng, Yanhua; Xi, Yixuan; Qin, Lianmei; Chen, Yiwen; Xiu, Feichen; Liu, Lu] Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang Med Sch,Inst Pathogen Biol, Hengyang, Peoples R China.;[Wu, Yueping; Qin, Lianmei] Shenzhen Childrens Hosp, Dept Blood Transfus, Shenzhen, Peoples R China.;[Luo, Haodang; He, Jun] Univ South China, Hengyang Med Sch, Affiliated Nanhua Hosp, Dept Clin Lab, Hengyang, Peoples R China.;[Hu, Jun] Univ South China, Hengyang Med Sch, Affiliated Hosp 2, Dept Cardiothorac Surg, Hengyang, Peoples R China.;[Wu, Ning] Hengyang 1 Peoples Hosp, Dept Clin Lab, Hengyang, Peoples R China.
通讯机构:
[Xiaoxing You] I;Institute of Pathogenic Biology, Hengyang Medical School, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China
关键词:
Mycoplasma pneumoniae;matrix metalloproteinase-9;reversion-inducing cysteine-rich protein with Kazal motifs (RECK);bronchial epithelial cell
作者机构:
[You Xiaoxing] Univ South China, Hengyang Med Sch, Inst Pathogen Biol, Hengyang, Peoples R China.;Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang, Peoples R China.;Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang, Peoples R China.
通讯机构:
[You Xiaoxing] I;Institute of Pathogenic Biology, Hengyang Medical School<&wdkj&>Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control<&wdkj&>Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China
摘要:
Mycoplasma genitalium, a pathogen from class Mollicutes, has been linked to sexually transmitted diseases and sparked widespread concern. To adapt to its environment, M. genitalium has evolved specific adhesins and motility mechanisms that allow it to adhere to and invade various eukaryotic cells, thereby causing severe damage to the cells. Even though traditional exotoxins have not been identified, secreted nucleases or membrane lipoproteins have been shown to cause cell death and inflammatory injury in M. genitalium infection. However, as both innate and adaptive immune responses are important for controlling infection, the immune responses that develop upon infection do not necessarily eliminate the organism completely. Antigenic variation, detoxifying enzymes, immunoglobulins, neutrophil extracellular trap-degrading enzymes, cell invasion, and biofilm formation are important factors that help the pathogen overcome the host defence and cause chronic infections in susceptible individuals. Furthermore, M. genitalium can increase the susceptibility to several sexually transmitted pathogens, which significantly complicates the persistence and chronicity of M. genitalium infection. This review aimed to discuss the virulence factors of M. genitalium to shed light on its complex pathogenicity and pathogenesis of the infection.
通讯机构:
[Chen, L.] I;Institute of Pharmacy and Pharmacology, Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hunan, Hengyang, China
关键词:
ER-phagy;FAM134B;Cancer;HSAN II B;Viral diseases;Vascular dementia
作者机构:
[Lv, Deguan; Luo, Xuling; Zhou, Qionglin; Tang, Mingzhu; Jiang, Jinyong; Li, Lanfang; Liu, Jiaqi; Lu, Liqun; Chen, Zhe; Chen, Linxi] Univ South China, Coll Basic Med Sci, Hengyang Med Coll, Inst Pharm & Pharmacol, Hengyang, Peoples R China.;[Lv, Deguan] Univ Calif San Diego, Dept Med, Div Regenerat Med, San Diego, CA 92103 USA.;[Zhong, Cuiqing] Shanghai Jiao Tong Univ, Ren Ji Hosp, Ctr Reprod Med, Sch Med, Shanghai, Peoples R China.;[Liu, Meiqing] Kunming Med Univ, Dept Pharm, YanAn Hosp, Kunming, Yunnan, Peoples R China.;[Li, Yao] Yueyang Maternal & Child Hlth Care Hosp, Dept Pharm, Yueyang, Peoples R China.
通讯机构:
[Cuiqing Zhong] C;[Lanfang Li; Linxi Chen] I;Institute of Pharmacy and Pharmacology, College of Basic Medical Science, Hengyang Medical College, University of South China, Hengyang, China<&wdkj&>Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China<&wdkj&>Gene Expression Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
摘要:
Nature Reviews' clinical content is written by internationally renowned clinical academics and researchers and targeted towards readers in the medical sciences, from postgraduate level upwards. While intended to be read by practicing doctors, researchers and academics within a specialty, we aim to make all our articles accessible to readers working in any medical discipline. In-depth Reviews present authoritative, up-to-date information on a topic, placing it in the context of a field's history and development. Topical discussion and opinions are proffered in Perspectives and News & Views articles, and in the Research Highlights section we filter primary research from a range of specialty and general medical journals. Finally, Case Studies enable authors to present novel and interesting cases and discuss the most useful features to bear in mind for treating future cases.Subjects coveredRheumatoid arthritisOsteoarthritisMetabolic bone disease (including osteoporosis, osteonecrosis, Paget disease of bone)Connective tissue diseases (including systemic lupus erythematosus, scleroderma (systemic sclerosis), antiphospholipid antibody disease, S�gren disease, Raynaud phenomenon, Cogan keratitis syndrome, relapsing polychondritis) Spondyloarthropathies (including ankylosing spondylitis, reactive arthritis, psoriatic arthritis, arthritis of inflammatory bowel disease)Acute inflammatory arthritis (including monoarthritis, oligoarthritis, polyarthritis, infectious/septic arthritis, crystal deposition diseases, Lyme disease)The idiopathic inflammatory myopathies (including polymyositis, dermatomyositis, juvenile dermatomyositis, inclusion body myositis)FibromyalgiaPolymyalgia RheumaticaVasculitis syndromes (including systemic vasculitides, large, medium and small vessel vasculitides, vasculitis of small vessels)Periarticular rheumatic disease (including bursitis, tendonitis)Pediatric rheumatic diseaseMiscellaneous rheumatic diseasesTherapy (including pharmacotherapy, physical therapy [exercise and rest], surgery, splinting, complementary and alternative treatments)Pain managementRehabilitationEpidemiologyGeneticsImmunologyInflammationHealth services, economics and outcomes research
期刊:
Journal of Medical Virology,2021年93(10):5825-5832 ISSN:0146-6615
通讯作者:
Wang, S.;Wang, X.
作者机构:
[Zhou, Zhe; Yang, Peng; Wang, Xuejun; Wu, Yuming; Huang, Cong; Wang, Shengqi; Yang, Yang] Beijing Inst Radiat Med, Beijing 100850, Peoples R China.;[Yang, Peng; Wang, Shengqi] Henan Normal Univ, Coll Life Sci, Xinxiang, Henan, Peoples R China.;[Huang, Cong] Univ South China, Cooperat Innovat Ctr Mol Target New Drug Study, Hengyang, Peoples R China.
通讯机构:
[Xuejun Wang; Shengqi Wang] B;Beijing Institute of Radiation Medicine, Beijing, China<&wdkj&>Beijing Institute of Radiation Medicine, Beijing, China<&wdkj&>College of Life Sciences, Henan Normal University, Xinxiang, China
期刊:
Life Sciences,2021年271(1):119181 ISSN:0024-3205
通讯作者:
Chunxue Lu
作者机构:
[Li, Zhongyu; Lu, Chunxue; Li, Yumeng; Chen, Hui; Chen, Lili; Xie, Lijuan; Luan, Xiuli; Wang, Chuan; Sun, Zhenjie; Xiao, Jian] Univ South China, Hengyang Med Coll, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pathogen Biol,Hunan Prov Key Lab Special Pat, Hengyang 421001, Peoples R China.;[Peng, Bo] Univ South China, Hengyang Med Coll, Inst Pathogen Biol, Hengyang 421001, Peoples R China.
通讯机构:
[Chunxue Lu] I;Institute of Pathogenic Biology, Hengyang Medical College, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang 421001, China
摘要:
Nature Reviews' clinical content is written by internationally renowned clinical academics and researchers and targeted towards readers in the medical sciences, from postgraduate level upwards. While intended to be read by practicing doctors, researchers and academics within a specialty, we aim to make all our articles accessible to readers working in any medical discipline. In-depth Reviews present authoritative, up-to-date information on a topic, placing it in the context of a field's history and development. Topical discussion and opinions are proffered in Perspectives and News & Views articles, and in the Research Highlights section we filter primary research from a range of specialty and general medical journals. Finally, Case Studies enable authors to present novel and interesting cases and discuss the most useful features to bear in mind for treating future cases.Subjects coveredRheumatoid arthritisOsteoarthritisMetabolic bone disease (including osteoporosis, osteonecrosis, Paget disease of bone)Connective tissue diseases (including systemic lupus erythematosus, scleroderma (systemic sclerosis), antiphospholipid antibody disease, S�gren disease, Raynaud phenomenon, Cogan keratitis syndrome, relapsing polychondritis) Spondyloarthropathies (including ankylosing spondylitis, reactive arthritis, psoriatic arthritis, arthritis of inflammatory bowel disease)Acute inflammatory arthritis (including monoarthritis, oligoarthritis, polyarthritis, infectious/septic arthritis, crystal deposition diseases, Lyme disease)The idiopathic inflammatory myopathies (including polymyositis, dermatomyositis, juvenile dermatomyositis, inclusion body myositis)FibromyalgiaPolymyalgia RheumaticaVasculitis syndromes (including systemic vasculitides, large, medium and small vessel vasculitides, vasculitis of small vessels)Periarticular rheumatic disease (including bursitis, tendonitis)Pediatric rheumatic diseaseMiscellaneous rheumatic diseasesTherapy (including pharmacotherapy, physical therapy [exercise and rest], surgery, splinting, complementary and alternative treatments)Pain managementRehabilitationEpidemiologyGeneticsImmunologyInflammationHealth services, economics and outcomes research
摘要:
Listeria monocytogenes, a fatal foodborne pathogen has the extraordinary capacity to survive in harsh conditions and is a potential threat to public health. A novel 91 kb plasmid pLM1686 was found in the prevalent L. monocytogenes sequence type (ST) 87 strain in China. In this study, the function and distribution of pLM1686 were firstly investigated in L. monocytogenes. The results showed plasmid pLM1686 had self-transmissible ability and existed in various types of L. monocytogenes isolates belonging to two lineages (lineage I and II), four serotypes (1/2b, 3b, 1/2c and 1/2a) and four STs (ST87, ST59, ST9 and ST120). The wild strain LM1686 and transconjugant strain 10403SP1686 exhibited significantly higher growth rate and biofilm formation in Modification of Welshimer's medium (MWB), greater salinity tolerance, stronger cell invasion and higher cytotoxicity than plasmid-cured strain and reference strain 10403S. Moreover, plasmid curing caused the loss of cadmium resistance of strain, and the recipient strain acquired cadmium resistance after conjugation. Thus, pLM1686 would provide L. monocytogenes advantages of surviving in adverse environments.
作者机构:
[Wu, Yueyue; Qin, Lianmei; Chen, Yiwen; Zhu, Cuiming; You, Xiaoxing] Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pathogen Biol,Hengyang Med Coll, Hengyang, Peoples R China.
通讯机构:
[You Xiaoxing] I;Institute of Pathogenic Biology, Hengyang Medical College, University of South China<&wdkj&>Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control<&wdkj&>Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, China
摘要:
Mycoplasmas, the smallest bacteria lacking a cell wall, can cause various diseases in both humans and animals. Mycoplasmas harbor a variety of virulence factors that enable them to overcome numerous barriers of entry into the host; using accessory proteins, mycoplasma adhesins can bind to the receptors or extracellular matrix of the host cell. Although the host immune system can eradicate the invading mycoplasma in most cases, a few sagacious mycoplasmas employ a series of invasion and immune escape strategies to ensure their continued survival within their hosts. For instance, capsular polysaccharides are crucial for anti-phagocytosis and immunomodulation. Invasive enzymes degrade reactive oxygen species, neutrophil extracellular traps, and immunoglobulins. Biofilm formation is important for establishing a persistent infection. During proliferation, successfully surviving mycoplasmas generate numerous metabolites, including hydrogen peroxide, ammonia and hydrogen sulfide; or secrete various exotoxins, such as community-acquired respiratory distress syndrome toxin, and hemolysins; and express various pathogenic enzymes, all of which have potent toxic effects on host cells. Furthermore, some inherent components of mycoplasmas, such as lipids, membrane lipoproteins, and even mycoplasma-generated superantigens, can exert a significant pathogenic impact on the host cells or the immune system. In this review, we describe the proposed virulence factors in the toolkit of notorious mycoplasmas to better understand the pathogenic features of these bacteria, along with their pathogenic mechanisms.
期刊:
Life Sciences,2020年263(1):118579- ISSN:0024-3205
通讯作者:
Li, Zhongyu
作者机构:
[Luo, Fangzhen; Li, Zhongyu; Chen, Hongliang; Wen, Yating] Univ South China, Hengyang Med Coll, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Inst Pathogen Biol,Hunan Prov Cooperat Innovat Ct, Hengyang 421001, Peoples R China.;[Chen, Hongliang] Chenzhou 1 Peoples Hosp, Dept Clin Microbiol Lab, Chenzhou 423000, Peoples R China.;[Zhou, Hui] Hunan Univ Chinese Tradit Med, Dept Lab Med, Affiliated Hosp 1, Changsha 410007, Peoples R China.;[Li, Zhongyu] Univ South China, Hengyang Med Coll, Pathogen Biol Inst, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Li, Zhongyu] U;Univ South China, Hengyang Med Coll, Pathogen Biol Inst, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.
关键词:
Long noncoding RNAs (lncRNAs);Epigenetic regulation;Host-pathogen interaction;Bacterial infection;Antibacterial immunity
摘要:
Bacteria can induce significant alteration in the cell transcriptome and develop many strategies to modify immune signaling for its survival. In recent years, a new class of regulatory RNAs, long noncoding RNAs (lncRNAs), has been demonstrated to play an essential role in host gene expression. Growing literature indicate that lncRNAs function as positive or negative effectors on antibacterial immunity. On the one hand, the host regulates immune-related genes at epigenetic, transcriptional, and post-transcriptional levels by lncRNAs, thereby protecting itself from pathogen invasion. On the other hand, bacteria can manipulate the host signaling pathways by regulating the host lncRNAs to escape immune clearance. In addition, some bacteria even produce lncRNAs, which are involved in the pathogenic process of pathogens. Some dysregulated lncRNAs during bacterial infections can be used as a potential diagnostic marker for infection. Understanding of gene expression regulation through lncRNAs helps illustrate bacterial pathogenesis. Here, we summarize the functions of lncRNAs and current advances of lncRNAs in different bacterial infections and look forward to the future research orientation.
摘要:
This study aims to identify differentially expressed microRNAs (miRNAs) in gastric cancer by comparing gastric cancerous tissues with normal tissues, explore the potential roles.The miRNA expression microarray was employed on gastric cancer tissues, and apparently normal para-cancerous tissues from 3 patients undergoing radical surgery were matched. Quantitative RT-PCR was performed on the other 7 patients to validate the findings of the microarray. Furthermore, Gene Ontology (GO) analysis and enrichment analysis of KEGG Pathway were performed for 5 dysregulated candidate miRNAs, including 3 upregulated (miR-31-3p, miR-6736-3p, and miR-147b) and 2 downregulated (miR-3065-5p and miR-3921) miRNAs, in order to determine the role of miRNAs in tumorigenesis and development.Among these miRNAs, 17 miRNAs were found to be upregulated, and 19 miRNAs were found to be downregulated. The dysregulated expression of 5 candidate miRNAs, including miR-31-3p, miR-147b, miR-6736-3p, miR-3065-5p, and miR-3921, were verified by quantitative RT-PCR in the validation set. Among these miRNAs, miR-31-3p, miR-6736-3p, miR-3065-5p, and miR-3921 had 551 target gene intersections. The GO and KEGG Pathway analyses Revealed that miR-31-3p, miR-6736-3p, miR-3065-5p, and miR-3921 may participate in multiple pathophysiological processes, such as foreign substance metabolism and chemical carcinogenesis.The profile of differentially expressed miRNAs was successfully screened, and 4 miRNAs (i.e., miR-31-3p, miR-6736-3p, miR-3065-5p, and miR-3921) appeared to be involved in gastric carcinogenesis. These might serve as promising biomarkers for gastric cancer.
摘要:
Amino acid derivatives containing chrysin were synthesized for evaluating their anticancer effects. Among them, compound N-(7-((5-hydroxy-4-oxo-2-phenyl-4H-chromen-7-yl)oxy)heptanoyl)-L-isoleucine (6e) displayed the most potent antiproliferative activity against MGC-803 cells with IC50 value of 20.0M. Preliminary mechanistic studies showed that compound 6e could inhibit the colony formation and migration of MGC-803 cells. Flow cytometry analysis demonstrated that compound 6e mediated cell apoptosis of MGC-803 cells and arrested cell cycle in G2/M-phase. Moreover, 6e treatment in MGC-803 cells downregulated anti-apoptotic protein Bcl-2 and upregulated pro-apoptotic gene Bax in a concentration-dependent manner. Our studies suggest that compound 6e may sever as an effective chemotherapeutic candidate. [GRAPHICS] .