摘要：
Here, we report a new approach of on-resin peptide ligation using C-terminal benzyl ester as the stabilized precursor of thioester, which enables both N-terminal elongation and C-terminal peptide ligation on a Rink Amide resin. On-resin native chemical ligation and auxiliary-assisted peptide ligation were successfully achieved. This method is compatible to both protected and unprotected peptide fragments and has potential application in poor water-soluble peptide ligation.
作者机构：
[Tang Mingzhu; Lu Liqun; Huang Zhen; Chen Linxi] Institute of Pharmacy and Pharmacology, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China,Learning Key Laboratory for Pharmacoproteomics, Hengyang, 421001
通讯机构：
[Chen, L.] I;Institute of Pharmacy and Pharmacology, Learning Key Laboratory for Pharmacoproteomics, University of South China, Hengyang, China
关键词：
线粒体;动力学;机制;病理;信号;蛋白质;关键酶;荧光灯
摘要：
Acta Biotheoretica is devoted to the promotion of theoretical biology, encompassing mathematical biology and the philosophy of biology, paying special attention to the methodology of formation of biological theory. Papers on all kind of biological theories are welcome. Interesting subjects include philosophy of biology, biomathematics, computational biology, genetics, ecology and morphology. The process of theory formation can be presented in verbal or mathematical form. Moreover, purely methodological papers can be devoted to the historical origins of the philosophy underlying biological theories and concepts. Papers should contain clear statements of biological assumptions, and where applicable, a justification of their translation into mathematical form and a detailed discussion of the mathematical treatment. The connection to empirical data should be clarified. Acta Biotheoretica also welcomes critical book reviews, short comments on previous papers and short notes directing attention to interesting new theoretical ideas. Coverage in the Journals@Ovid database begins with the first 1997 issue.
作者机构：
[Liqun Lu; Mingzhu Tang] Institute of Pharmacy and Pharmacology, Learning Key Laboratory for Pharmacoproteomics, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, 421001, China;[Feng Xie] Institute of Pharmacy and Pharmacology, Learning Key Laboratory for Pharmacoproteomics, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, 421001, China. 764136587@qq.com;[Linxi Chen] Institute of Pharmacy and Pharmacology, Learning Key Laboratory for Pharmacoproteomics, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, 421001, China. lxchen6@126.com
通讯机构：
[Feng Xie; Linxi Chen] I;Institute of Pharmacy and Pharmacology, Learning Key Laboratory for Pharmacoproteomics, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China<&wdkj&>Institute of Pharmacy and Pharmacology, Learning Key Laboratory for Pharmacoproteomics, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China
关键词：
Critical Mechanism;Neuronal Function;Fragile X Mental Retardation Protein (FMRP);Small Ubiquitin-like Modifier (SUMO);methyl-CpG-binding Protein 2 (MeCP2)
摘要：
Recently,Khayachi et al.showed that fragile X mental retardation protein (FMRP) is an active substrate of the small ubiquitin-like modifier (SUMO) pathway in neurons.FMRP SUMOylation is induced by the activation of metabotropic glutamate receptors (mGlu5Rs).FMRP SUMOylation is required for dissociating FMRP from dendritic RNA granules and controlling spine density and proper maturation.Mechanically,the SUMOylation process is triggered by the activation of mGlu5Rs,thereby contributing to maintaining FMRP-mediated neuronal function.In fact,some proteins that mediate synaptic plasticity,neurotransmitter release,and neuronal network formation,are mostly regulated by SUMOylation.Therefore,SUMOylation has emerged as an essential posttranslational modification in the nervous system.This novel discovery first provides evidence to support the idea that FMRP is a novel substrate of SUMOylation and acts as an essential regulator in the developing brain.Clearly,it also expands the biological ramifications of FMRP modification,since FMRP was previously supposed to be modulated by phosphorylation and dephosphorylation.Together,their research reveals that the rapid SUMOylation of FMRP results from the activation of mGlu5Rs and activity-dependent FMRP SUMOylation is a novel target for affecting neuronal functions.
作者机构：
[Tang, SS; Mo, Zhongcheng; Ou, Hanxiao; Tang, Shengsong; Liu, Chuhao; Xiao, Xinwen; Feng, Wenjie] Univ South China, Inst Pharm & Pharmacol, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.;[Tang, Shengsong] Hunan Univ Med, Ctr Life Sci, Huaihua 418000, Peoples R China.;[Liu, Chuhao; Xiao, Xinwen; Feng, Wenjie] Univ South China, Med Sch, Hengyang 421001, Peoples R China.
通讯机构：
[Tang, SS; Mo, ZC] U;[Tang, Shengsong] H;Univ South China, Inst Pharm & Pharmacol, Clin Anat & Reprod Med Applicat Inst, Hengyang 421001, Peoples R China.;Hunan Univ Med, Ctr Life Sci, Huaihua 418000, Peoples R China.
关键词：
adenosine monophosphate-activated protein kinase;autophagy;lipid metabolism;atherosclerosis-associated cell;atherosclerosis
摘要：
Atherosclerosis is characterized by the accumulation of lipids and deposition of fibrous elements in the vascular wall, which is the primary cause of cardiovascular diseases. Adenosine monophosphate-activated protein kinase (AMPK) is a metabolic sensor of energy metabolism that regulates multiple physiological processes, including lipid and glucose metabolism and the normalization of energy imbalances. Overwhelming evidence indicates that AMPK activation markedly attenuates atherosclerosis development. Autophagy inhibits cell apoptosis and inflammation and promotes cholesterol efflux and efferocytosis. Physiological autophagy is essential for maintaining normal cardiovascular function. Increasing evidence demonstrates that autophagy occurs in developing atherosclerotic plaques. Emerging evidence indicates that AMPK regulates autophagy via a downstream signaling pathway. The complex relationship between AMPK and autophagy has attracted the attention of many researchers because of this close relationship to atherosclerosis development. This review demonstrates the role of AMPK and autophagy in atherosclerosis. An improved understanding of this interrelationship will create novel preventive and therapeutic strategies for atherosclerosis.
摘要：
Bacterial quorum sensing(QS) molecules are involved in the coordination of certain behaviors such as biofilm formation, virulence and antibiotic resistance. QS molecules(autoinducers) and their corresponding receptors have been recognized as important therapeutic targets for drug-resistant infections and biofilm-associated infections(BAI). This study assessed the multiple biological effects of homogentisic acid y-lactone(HgAL), a furanone derivative. The anti-QS and anti-biofilm effects of HgAL against PAO1 strain were evaluated using CLSM, SEM, HPLC and other biochemical methods The results showed that HgAL could effectively inhibit the production of pyocyanin and extracellular matrix, as well as reduce the adherence ability and biofilm formation of Pseudomonas aeruginosa. Inhibition of virulence is attributed to the suppressive effect of HgAL on biosynthesis of 3-oxo-C_(12)-HSL and C_4-HSL(two kinds of QS signaling molecules in P. aeruginosa). Our results support HgAL as a potential agent for prevention of BAI in the healthcare settings.
作者：
Qionglin Zhou;Kai Zhang;Yu Guo;Linxi Chen;Lanfang Li
期刊：
生物化学与生物物理学报,2018年50(3):319-321 ISSN：1672-9145
通讯作者：
Chen, L.
作者机构：
[Zhou Qionglin; Zhang Kai; Guo Yu; Chen Linxi; Li Lanfang] Institute of Pharmacy and Pharmacology, University of South China, Learning Key Laboratory for Pharmacoproteomics;[Zhou Qionglin; Zhang Kai; Guo Yu; Chen Linxi; Li Lanfang] Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, 421001
通讯机构：
[Chen, L.] I
关键词：
apoptosis;APELIN
摘要：
APJ is a seven-transmembrane G protein coupled receptor. Apelin is an endogenous ligand of APJ [1]. It is well known that Apelin and APJ receptor are widely distributed in a variety of organs including the heart, brain, kidney, stomach, lung, adipose tissues, endothelium, vascular smooth muscle cells, testis, ovary, and gland, particularly in the cardiovascular system. The Apelin/APJ system plays multiple important roles in various physiological and pathological processes such as regulation of blood pressure, cardiac contractility, water homeostasis, immunity, glucose metabolism, fat metabolism, inflammation, liver fibrosis, cardiovascular development, apoptosis, revascularization, as well as cell proliferation.
作者机构：
[Tang M.; Luo X.; Huang Z.; Chen L.] Learning Key Laboratory for Pharmacoproteomics, Institute of Pharmacy and Pharmacology, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, 421001, China
通讯机构：
[Huang, Z.] L;Learning Key Laboratory for Pharmacoproteomics, Institute of Pharmacy and Pharmacology, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China
关键词：
APOPTOSIS
摘要：
Mitochondria are dynamic, double-membrane-bound organelle of eukaryotic cells that have evolved to function in a wide range of cellular processes. They are responsible for oxidative phosphorylation (OXPHOS), producing energy and metabolites, regulating apoptosis and maintaining cell viability. To properly perform these functions, mitochondria depend on hundreds of proteins which are encoded in the nucleus, synthesized in the cytosol, and imported into the organelle. The transport of mitochondrial preproteins is mainly mediated by the translocase of the outer mitochondrial membrane and the translocase of the inner mitochondrial membrane [1]. Given the dependence of mitochondrial function on cytosolic synthesized proteins, maintaining efficient mitochondrial protein import is indispensable for cellular and organismal health. Therefore, it is not surprising that some sophisticated protective responses must exist to defend against deficient mitochondrial protein import in cells.
作者机构：
[CHEN Zhe; HUANG Zhen; CHEN Lin Xi] Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, Hunan, China
通讯机构：
[HUANG, Z.] I;Institute of Pharmacy and Pharmacology, University of South China, Hengyang, Hunan, China
关键词：
嗅觉;受体;治疗学;疾病;神经原;醋酸;基因;果蝇
摘要：
Recently, Prieto-Godino et al. found that the olfactory receptor 75a (Ir75a) gene is a functional pseudo-pseudogene in Drosophila sechellio. For a long time, Ir75a has been regarded as an acetic acid receptor that detects acetic acid and induces obvious olfactory responses in olfactory sensory neurons (OSNs). Nonetheless, Prieto-Godino et al. confirmed that Ir75a lost its sensitivity to acetic acid in D. sechellia. Thus, the D. sechellia lr75a gene is generally recognized as a pseudogene in OSNs. Nevertheless, the D. sechellia Ir75a gene is not a simple pseudogene. Prieto-Godino et al. found that D. sechellia Ir75a is sensitive to propionic, butyric, and 2-oxopentanoic acids. Therefore, the D. sechellia Ir75a gene encodes a functional olfactory receptor (OR) that induces different olfactory responses.
