摘要：
Natural and artificial nucleases have extensive applications in biotechnology and biomedicine. The exploration of protein with potential DNA cleavage activity also inspires the design of artificial nuclease and helps to understand the physiological process of DNA damage. In this study, we engineered four human cytochrome c (Cyt c) mutants (N52S, N52A, I81N, and I81D Cyt c), which showed enhanced DNA cleavage activity and degradation in comparison with WT Cyt c, especially under acidic conditions. The mechanism assays revealed that the superoxide (O2 & BULL; ) plays an important role in the nuclease reaction. The kinetic assays showed that the peroxidase activity of the I81D Cyt c mutant enhanced up to 9-fold at pH 5. This study suggests that the mutations of Ile81 and Asn52 in & omega;-loop C/D are critical for the nuclease activity of Cyt c, which may have physiological significance in DNA damage and potential applications in biomedicine.
摘要：
Abstract A copper‐catalyzed direct C(sp2)−H bond aminosulfonylation of maleimides is demonstrated. This protocol enables concurrent construction of C(sp2)−N and C(sp2)−SO2 bonds in one‐step and features high efficiency, broad substrate scopes, good functional‐group tolerance, and mild reaction conditions. Preliminary mechanistic studies indicate that the reaction probably involves a radical way. Significantly, this method is applicable to synthesize derivatives of pharmaceuticals such as Desloratadine, Fluoxetine, Atomoxetine, and Maprotiline.
作者：
Hu, Rong;Zhao, Zheng;Liu, Zhuang;Tang, Ben Zhong
期刊：
Biomaterials,2023年297:122119 ISSN：0142-9612
通讯作者：
Zhao, Zheng(zhaozheng@cuhk.edu.cn);Liu, Zhuang(zliu@suda.edu.cn);Tang, Ben Zhong(tangbenz@cuhk.edu.cn)
作者机构：
[Hu, Rong] State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou;510640, China;School of Chemistry and Chemical Engineering, University of South China, Hengyang;421001, China;[Zhao, Zheng; Tang, Ben Zhong] School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Guangdong, Shenzhen
通讯机构：
[Long, SS; Lin, YW ] U;[Deng, F ] J;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;Jinggangshan Univ, Sch Chem & Chem Engn, Jian 343009, Jiangxi, Peoples R China.
摘要：
The development of organelle-targeted probes that enable specific recognition and detection Cysteine (Cys) may provide an effective way to explore physiological processes and pathological mechanisms associated with the related diseases. In this work, an acetylpyrene-based fluorescent probe CP-1 was designed and syn-thesized for detecting Cys in vitro and in vivo. The probe exhibited a noticeable fluorescence blue shift upon the addition of Cys under UV light, attributed to the mechanism of the nucleophilic addition reaction. The response mechanism for Cys was studied by time-dependent density functional theory (TD-DFT) calculations and mass spectra (MS). CP-1 can not only specifically recognize Cys in the presence of other amino acids and biothiols, but also exhibit the property of targeting lipid droplets (LDs). Due to the good cell biocompatibility, CP-1 has been successfully employed for real-time tracking Cys in LDs of living cells. The ability to target LDs and the favorable biocompatibility make the probe valuable for investigating Cys-related diseases in living cell systems.
作者机构：
[Li, Yuqing; Jin, Bowen; Liu, Yihong; Gao, Xinyu; Chen, Wansong; Jiang, Peixi; Liu, You-Nian; Li, Jianghua] Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, China;[Chen, Xi] Department of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China;[Wei, Chuanwan] School of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China;[Sheng, Jianping] School of Optoelectronic Science and Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan 611731, China
摘要：
Catalytic nanomedicine can in situ catalytically generate bactericidal species under external stimuli to defend against bacterial infections. However, bacterial biofilms seriously impede the catalytic efficacy of traditional nanocatalysts. In this work, MoSe(2) nanoflowers (NFs) as piezoelectric nanozymes were constructed for dual-driven catalytic eradication of multi-drug-resistant bacterial biofilms. In the biofilm microenvironment, the piezoelectricity of MoSe(2) NFs was cascaded with their enzyme-mimic activity, including glutathione oxidase-mimic and peroxidase-mimic activity. As a result, the oxidative stress in the biofilms was sharply elevated under ultrasound irradiation, achieving a 4.0 log(10) reduction of bacterial cells. The in vivo studies reveal that the MoSe(2) NFs efficiently relieve the methicillin-resistant Staphylococcus aureus bacterial burden in mice under the control of ultrasound at a low power density. Moreover, because of the surface coating of antioxidant poly(ethyleneimine), the dual-driven catalysis of MoSe(2) NFs was retarded in normal tissues to minimize the off-target damage and favor the wound healing process. Therefore, the cascade of piezoelectricity and enzyme-mimic activity in MoSe(2) NFs reveals a dual-driven strategy for improving the performance of catalytic nanomaterials in the eradication of bacterial biofilms.
摘要：
The sequence and structure of human cytochrome c (hCyt c) exhibit evolutionary conservations, with only a limited number of naturally occurring mutations in humans. Herein, we investigated the effects of the naturally occurring S47F/A mutations on the structure and function of hCyt c in the oxidized form. Although the naturally occurring S47F/A mutations did not largely alter the protein structure, the S47F and S47A variants exhibited a small fraction of high-spin species. Kinetic studies showed that the peroxidase activity of the variants was enhanced by ∼2.5-fold under neutral pH conditions, as well as for the rate in reaction with H(2)O(2), when compared to those of wild-type hCyt c. In addition, we evaluated the interaction between hCyt c and human neuroglobin (hNgb) by isothermal titration calorimetry (ITC) studies, which revealed that the binding constant was reduced by ∼8-fold as result of the mutation of the hydrophilic Ser to the hydrophobic Phe/Ala. These findings provide valuable insights into the role of Ser47 in Ω-loop C in sustaining the structure and function of hCyt c.
摘要：
In this study, a crystal material UIO-66-NH2-BPDA was designed by grafting BPDA onto UIO-66-NH2 and applied to extract U(VI) from aqueous solution. According to performance tests, UIO-66-NH2-BPDA exhibits excellent chemical and thermal stability. The experimental results show that U(VI) adsorption by UIO-66-NH2-BPDA was tested to be 346.58 mg g(-1) at pH = 4 and T = 298 K. The data simulation demonstrates that the adsorption process was consistent with Langmuir isotherm and pseudo-second-order model. Meanwhile, the thermodynamic parameters suggest that the adsorption process was spontaneous and endothermic. Most noteworthy is the good selectivity for U(VI) adsorption by UIO-66-NH2-BPDA. It can be seen that UIO-66-NH2-BPDA can be used as a potential effective adsorbent to remove U(VI) from wastewater.
摘要：
The electron transport layer (ETL) plays a crucial role in achieving high performance and stability of organic solar cells (OSCs). ETL materials suffering from low conductivity can impede charge collection and transport. Hyperbranched polymers display advantages of excellent film-forming property and facile preparation, which, however, often show low conductivity. In this work, we designed two hyperbranched polymers, HPDIN-B01 and HPDIN-B02, integrating the PDIN segments using two tribromomethylbenzene cores through a green and environmentally-friendly quaternization polymerization reaction. Both hyperbranched polymers possess outstanding alcohol solubility and suitable energy levels. Notably, HPDIN-B02 bearing three ethyl units on the benzene core exhibits a strong self-doping behavior as confirmed by electron spin resonance measurements, which is favorable for charge extraction and transport. As a result, when using HPDIN-B02 as ETL, PM6:L8-BO-based OSCs delivered a high power conversion efficiency (PCE) of 18.62%, and the performance is more insensitive to the thickness of the HPDIN-B02 film compared to that using HPDIN-B01. More importantly, HPDIN-B02-based devices also display remarkable durability under various conditions such as when stored in a glovebox, under thermal treatment, or under light illumination. This study demonstrates the great potential of the perylene diimide-based hyperbranched polymer as an efficient ETL for high-performing and stable OSCs.
作者机构：
[Li, Chun-Quan; Tang, Hui-Fang; Qian, Feng-Cui] Univ South China, Hengyang Med Sch, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.;[Li, Chun-Quan; Tang, Hui-Fang; Qian, Feng-Cui] Univ South China, Hengyang Med Sch, Hunan Prov Key Lab Multiom & Artificial Intelligen, Hengyang 421001, Hunan, Peoples R China.;[Li, Chun-Quan] Univ South China, Hengyang Med Sch, Hunan Prov Maternal & Child Hlth Care Hosp, Natl Hlth Commiss Key Lab Birth Defect Res & Preve, Hengyang 421001, Hunan, Peoples R China.;[Li, Chun-Quan; Qian, Feng-Cui] Univ South China, Sch Basic Med Sci, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.;[Li, Chun-Quan; Qian, Feng-Cui] Univ South China, Hengyang Med Sch, MOE Key Lab Rare Pediat Dis, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Li, CQ ; Tang, HF ; Tang, HF] U;Univ South China, Hengyang Med Sch, Affiliated Hosp 1, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hengyang Med Sch, Hunan Prov Key Lab Multiom & Artificial Intelligen, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hengyang Med Sch, Hunan Prov Maternal & Child Hlth Care Hosp, Natl Hlth Commiss Key Lab Birth Defect Res & Preve, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Sch Basic Med Sci, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.
摘要：
Chromatin accessibility profiles at single cell resolution can reveal cell type-specific regulatory programs, help dissect highly specialized cell functions and trace cell origin and evolution. Accurate cell type assignment is critical for effectively gaining biological and pathological insights, but is difficult in scATAC-seq. Hence, by extensively reviewing the literature, we designed scATAC-Ref (https://bio.liclab.net/scATAC-Ref/), a manually curated scATAC-seq database aimed at providing a comprehensive, high-quality source of chromatin accessibility profiles with known cell labels across broad cell types. Currently, scATAC-Ref comprises 1 694 372 cells with known cell labels, across various biological conditions, >400 cell/tissue types and five species. We used uniform system environment and software parameters to perform comprehensive downstream analysis on these chromatin accessibility profiles with known labels, including gene activity score, TF enrichment score, differential chromatin accessibility regions, pathway/GO term enrichment analysis and co-accessibility interactions. The scATAC-Ref also provided a user-friendly interface to query, browse and visualize cell types of interest, thereby providing a valuable resource for exploring epigenetic regulation in different tissues and cell types.
摘要：
Alkaline phosphatase (ALP) is a group of enzymes that catalyze hydrolysis of phosphate esters at an alkaline pH, resulting in the generation of inorganic phosphate. These enzymes are widely distributed, and their activity is found in various tissues including bone, liver, intestine, and placenta. However, abnormalities in ALP expression and activity have been observed in certain types of cancer. In some cases, elevated serum levels of ALP are observed in patients with liver and bone metastasis. In other cases, increased levels of ALP have been observed in patients with pancreatic and lung cancer. On the other hand, low expression of ALP has also been associated with poor prognosis in patients with certain types of tumors, including colorectal cancer (CRC), breast cancer, and non-small cell lung cancer (NSCLC). In these cases, low ALP activity may be associated with decreased differentiation of cancer cells and increased cancer cell proliferation. Overall, the role of ALP in cancer is complex and context-dependent. This article reviews application progress of ALP in cancer, and we hypothesize that ALP might be a potential tumor biomarker, combined detection of aspartate aminotransferase (AST)/alanine aminotransferase (ALT), bone-specific alkaline phosphatase (BSAP), carbohydrate antigen 19-9 (CA 19-9), lactate dehydrogenase (LDH) and ALP isozymes levels can be used for more accurate diagnosis of a particular tumor. Further research is needed to better understand the mechanisms underlying ALP dysregulation in cancer and to identify potential therapeutic targets.
作者机构：
[Yan, Qi; Song, Jiaqi; Wang, Jiyan; Chang, Hongkai; Sun, Huanran; Xie, Fei; Zhang, Han; Sun, Mingming; Shan, Changliang; Wang, Yingzhi; Qiao, Yaya] Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin Key Lab Mol Drug Res, Tianjin 300350, Peoples R China.;[Li, Zhen; Li, Leilei] Jinan Univ, Biomed Translat Res Inst, Guangzhou 510632, Peoples R China.;[Lin, Shu-Hai; Niu, Yujia] Xiamen Univ, Innovat Ctr Cell Signaling Network, Sch Life Sci, State Key Lab Cellular Stress Biol, Xiamen 361102, Peoples R China.;[Tan, Junzhen; Lai, Sizhen; Zhao, Huifang; Yang, Chenxin; Zhang, Shuai] Tianjin Univ Tradit Chinese Med, Sch Integrat Med, Tianjin 301617, Peoples R China.;[Li, Yanping] Jining Med Univ, Inst Precis Med, Dept Pathol, Jining 272067, Peoples R China.
通讯机构：
[Lin, S.-H.] S;State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, China
摘要：
Metabolic reprogramming is a hallmark of cancer, including lung cancer. However, the exact underlying mechanism and therapeutic potential are largely unknown. Here we report that protein argi-nine methyltransferase 6 (PRMT6) is highly expressed in lung cancer and is required for cell metabolism, tumorigenicity, and cisplatin response of lung cancer. PRMT6 regulated the oxidative pentose phosphate pathway (PPP) flux and glycolysis pathway in human lung cancer by increasing the activity of 6-phospho-gluconate dehydrogenase (6PGD) and a-enolase (ENO1). Furthermore, PRMT6 methylated R324 of 6PGD to enhancing its activity; while methylation at R9 and R372 of ENO1 promotes formation of active ENO1 dimers and 2-phosphoglycerate (2-PG) binding to ENO1, respectively. Lastly, targeting PRMT6 blocked the oxidative PPP flux, glycolysis pathway, and tumor growth, as well as enhanced the anti-tumor effects of cisplatin in lung cancer. Together, this study demonstrates that PRMT6 acts as a post -translational modification (PTM) regulator of glucose metabolism, which leads to the pathogenesis of lung cancer. It was proven that the PRMT6-6PGD/ENO1 regulatory axis is an important determinant of carcinogenesis and may become a promising cancer therapeutic strategy.(c) 2023 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
摘要：
Bis (diphenyl phosphate) imine (Htpop) was synthesized using a simple one‐step solvent method to remove thorium (IV) from aqueous solutions. The structure, functional group displacement, morphological differences, and elemental composition of the samples were analyzed using characterization techniques such as NMR, FTIR, SEM, EDS, and XPS. According to the batch experiment, when pH=4.0 and temperature was 298 K, the adsorption equilibrium can be reached within 40 min, and the adsorption capacity was 189.32 mg ⋅ g−1. The adsorption process conforms to the quasi‐second‐order kinetic model (R2=0.9959) and Langmuir model (R2=0.9971). Thermodynamic data (▵H0<0, ▵G0<0) indicate that the adsorption behavior of thorium (IV) in Htpop was spontaneous and exothermic. Abstract Bis (diphenyl phosphate) imine (Htpop) was synthesized using a simple one‐step solvent method to remove thorium (IV) from aqueous solutions. The structure, functional group displacement, morphological differences, and elemental composition of the samples were analyzed using characterization techniques such as NMR, FTIR, SEM, EDS, and XPS. According to the batch experiment, when pH=4.0 and temperature was 298 K, the adsorption equilibrium can be reached within 40 min, and the adsorption capacity was 189.32 mg ⋅ g−1. The adsorption process conforms to the quasi‐second‐order kinetic model (R2=0.9959) and Langmuir model (R2=0.9971). Thermodynamic data (▵H0<0, ▵G0<0) indicate that the adsorption behavior of thorium (IV) in Htpop was spontaneous and exothermic.
期刊：
Journal of Materials Science: Materials in Electronics,2023年34(36) ISSN：0957-4522
通讯作者：
Jin, LH
作者机构：
[Guo, Rongshuo; Zhang, Ye; Jin, Linghua; Yang, Huiyuan] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Wang, Weike] Hunan Normal Univ, Coll Phys & Informat Sci, Changsha 410081, Hunan, Peoples R China.
通讯机构：
[Jin, LH ] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
摘要：
The design and fabrication of transition metal sulfides for supercapacitors have attracted considerable attention in recent years because of their potential for energy storage applications. Conventionally, the synthesis process involved subjecting them to high temperatures and pressures, which can be energy-intensive and environmentally unfriendly. Herein, microcrystalline NiCo2S4 nanorods were prepared under ambient temperature and pressure using a two-step method. The Ni1/3Co2/3C2O4 nanorods were initially synthesized using the coprecipitation technique, and then the NiCo2S4 nanorods were obtained by sulfurating Ni1/3Co2/3C2O4 with Na2S<middle dot>9H(2)O under ambient conditions. The NiCo2S4 as a high-performance supercapacitor electrode has been demonstrated. The results exhibited that the prepared NiCo2S4 electrodes have low charge transfer resistance, and a high specific capacitance of 1330.0 F g(-1) at a current density of 1.0 A g(-1). Furthermore, a hybrid supercapacitor was assembled using activated carbon (AC) as the anode material and NiCo2S4 nanorods as the cathode material, and the resulting NiCo2S4//AC device exhibits an impressive energy density of 27.1 Wh kg(-1) at a power density of 1.6 kW kg(-1). This work demonstrates a vision for fabricating a microcrystalline NiCo2S4 cathode under ambient temperature and pressure for hybrid supercapacitors.
作者：
Ji, Hong-Tao;Wang, Ke-Li;Ouyang, Wen-Tao;Luo, Qing-Xia;Li, Hong-Xia;...
期刊：
Green Chemistry,2023年25(20):7983-7987 ISSN：1463-9262
通讯作者：
He, WM
作者机构：
[Li, Hong-Xia; Wang, Ke-Li; He, Wei-Min; Ji, Hong-Tao; Ouyang, Wen-Tao; Luo, Qing-Xia; He, WM] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
通讯机构：
[He, WM ] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
摘要：
An atom- and step-economical, efficient and eco-friendly method for constructing naphthoselenazol-2-amines through a visible-light photocatalytic multi-component reaction under aqueous phase conditions is reported. This visible-light-induced reaction proceeded at room temperature under exogenous photosensitizer- and additive-free, neutral and mild conditions with low-cost and abundant elemental selenium as the selenium source, ambient air as the clean oxidant and pure water as the sole solvent. An atom- and step-economical, efficient and eco-friendly method for constructing naphthoselenazol-2-amines through a visible-light photocatalytic multi-component reaction under aqueous phase conditions is reported.
通讯机构：
[Wang, Hongqing; Liao, Yun] H;[Wang, Meng] S;Hunan key laboratory for the design and application of actinide complexes, University of South China, Hengyang, Hunan 421001, PR China. Electronic address:;School of Nuclear Science and Technology, University of South China, Hengyang, Hunan 421001, China. Electronic address:;Hunan key laboratory for the design and application of actinide complexes, University of South China, Hengyang, Hunan 421001, PR China. Electronic address:
摘要：
As an energy-efficient and eco-friendly technique, capacitive deionization (CDI) has shown great potential for uranium (U(VI)) capture recently. However, extracting U(VI) with high kinetics, capacity and selectivity remains a major challenge due to the current surface active sites-based material and co-existing ions in aqueous solution. Here we rationally designed a layered 2D/2D niobium phosphate/holey graphene (HGNbP) electrode material, and originally demonstrated its efficient U(VI) capture ability via an electro-adsorption and electrocatalytic reduction coupling process. The less-accumulative loose layered architecture, open polycrystalline construction of niobium phosphate with active phosphate sites, and rich in-plane nano-pores on conductive graphene nanosheets endowed HGNbP with fast charge/ion transport, high electroconductivity and superior pseudocapacitance, which enabled U(VI) ions first to be electro-adsorbed, then physico-chemical adsorbed, and finally electrocatalysis reduced/deposited onto electrode surface without the limitation of active sites under a low potential of 1.2V. Based on these virtues, the HGNbP exhibited a fast adsorption kinetics, with a high removal rate of 99.9% within 30min in 50mgL(-1) U(VI) solution, and a high adsorption capacity up to 1340mgg(-1) in 1000mgL(-1) U(VI) solution. Furthermore, the good recyclability and selectivity towards U(VI) were also realized.
作者机构：
[郭帅帅; 金梁程龙; 陶醉; 彭国文] School of Resource & Environment and Safety Engineering, University of South China, Hunan, Hengyang;421001, China;[陈锦路; 陈小丽] School of Chemistry and Chemical Engineering, University of South China, Hunan, Hengyang;Engineering Technology Research Center for Decommissioning Treatment of Uranium Tailings Pond in Hunan Province, University of South China, Hunan, Hengyang;[郭帅帅; 陈锦路; 金梁程龙; 陶醉; 陈小丽; 彭国文] 421001, China
